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Method for extracting kinds of combined extract from blueberry stems and leaves

A technology of blueberry extract and composition, which is applied in the directions of pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of incomplete utilization of raw materials, high preparation and production cost, and achieve low product cost and resource utilization. High rate, good quality effect

Inactive Publication Date: 2016-04-20
张家界立功旅游农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these researches and inventions are insufficient in that they only focus on the extraction of a single active ingredient or component, the preparation and production costs are high, and the raw materials cannot be comprehensively utilized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Collect 10kg of pruned fresh green branches and leaves of the Southern Highbush blueberry Sharp Blue variety in September, cut into 1-3cm pieces and pieces for later use.

[0019] (2) Put the pretreated fresh blueberry stems and leaves into a 200L ultrasonic extraction tank, add 80kg of 60% ethanol, control the material load power to 500w / L, extract at a temperature of 40°C, stir and extract for 1 hour, suction filter, and microporous Filtrate, let the filtrate stand and settle overnight, take the supernatant, and adsorb it on AB-8 macroporous resin, the flow rate is 2BV / h, until the adsorption is saturated, the alcohol-water liquid flowing out of the upper column is collected for later use; elute with 2.4BV purified water , flow rate 1BV / h, until colorless after washing with water; then elute with 1.5BV of 40% ethanol, flow rate 1BV / h, collect the alcohol lotion, concentrate in vacuo, reclaim ethanol, and freeze-dry the concentrated solution to obtain 287g of bluebe...

Embodiment 2

[0023] (1) In December, collect 10kg of pruned red branches and leaves of the rabbit eye blueberry garden blue variety, cut into 1-3cm pieces and pieces, and dry them at a constant temperature of 60°C for later use.

[0024] (2) Put the pretreated dried stems and leaves of blueberries into a 200L microwave extraction tank, add 100kg of 75% ethanol, control the material load power to 350w / L, extract the temperature at 45°C, stir and extract for 1 hour, suction filter, microporous Filtrate, let the filtrate stand and settle overnight, take the supernatant, and adsorb it on D101 macroporous resin, the flow rate is 2BV / h, until the adsorption is saturated, and the alcohol-water liquid flowing out of the column is collected for later use; then eluted with 3.5BV purified water, Flow rate 1BV / h, until colorless after washing with water; then elute with 1.5BV of 50% ethanol at a flow rate of 1BV / h, collect the alcohol washing solution, concentrate in vacuo, recover ethanol, and freeze-...

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PUM

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Abstract

The invention provides a method for extracting kinds of combined extract from blueberry stems and leaves. The method includes the steps that fresh or dried blueberry branches and leaves are used as raw materials and coarsely crushed, a combined extraction technology including ethanol extraction, water extraction, enzyme hydrolysis, macroporous resin adsorption, membrane separation and the like is carried out in an ultrasonic wave or microwave assisted mode, and combined extract containing blueberry procyanidine, chlorogenic acid, polysaccharide and other active ingredients is prepared step by step. The method adapts to a wide raw material range, the materials are recycled in the production process, the technology is environmentally friendly, the resource use ratio is large, production cost is low, and product quality is good.

Description

technical field [0001] The invention relates to the technical field of comprehensive development and utilization of plant resources and extraction of active ingredients, and specifically provides a method for preparing various combined extracts from blueberry stems and leaves. Background technique [0002] Blueberry is the third-generation health-care fruit that has developed rapidly in China in recent years. The fruit is rich in nutrients such as fructose, organic acids, and vitamins, and various biologically active ingredients such as anthocyanins, anthocyanins, proanthocyanidins, quercetin, ellagic acid, chlorogenic acid, gallic acid, and polysaccharides, which are beneficial to human health. Double function of nutrition and health care. [0003] Blueberry is a shallow-rooted deciduous shrub with strong growth potential. In a growing season, the main stem can have multiple side branches growing, at least two new shoots are born, and the leaf buds germinate and shoot new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/45C08B37/00
CPCA61K36/45A61K2236/19A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53C08B37/00
Inventor 许玉泉陈国华侯志功王文茂
Owner 张家界立功旅游农业发展有限公司
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