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Rich-selenium black fungus fermentation slurry and preparation method and application thereof

A black fungus, selenium-enriched technology, applied in the directions of botanical equipment and methods, application, calcareous fertilizers, etc., can solve the problems of poor health care function, unstable sensory properties, and high additive content, and achieve balanced and stable nutrient components and avoid properties. Unstable, excellent lipid-lowering effect

Inactive Publication Date: 2016-04-20
HEBEI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a selenium-enriched black fungus fermented liquid and its preparation method and application to solve the problems of unstable sensory properties, high additive content, high cost, low safety and poor health care function in existing black fungus deep-processed drinks The problem

Method used

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  • Rich-selenium black fungus fermentation slurry and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0035](1) Cultivation of slant parent species: inoculate black fungus strain G6 on PDA slant medium, and prepare PDA slant medium according to: 20 grams of potato, 20 grams of glucose, 20 grams of agar, 1000 mL of water, and make PDA slant culture according to the routine base, inoculate black fungus strains on it, and cultivate at 28°C for 10 days to obtain black fungus female species;

[0036] (2) Erlenmeyer flask liquid fermentation: inoculate the female black fungus seed obtained in step (1) into a 500 ml triangular flask filled with 200 ml of selenium-enriched liquid medium, and inoculate 2 triangular flasks for each female seed, and cultivate it in selenium-enriched liquid medium The basic formula is: 20 grams of glucose, 2 grams of yeast extract, 2 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 60 milligrams of sodium selenite, 1000 milliliters of water; shaker speed 160 r / min, fermented and cultivated at 25 ° C for 6 days , to obtain fermentation...

Embodiment 2

[0043] (1) Cultivation of slant parent species: inoculate black fungus strain G6 on PDA slant medium, and prepare PDA slant medium according to: 20 grams of potato, 20 grams of glucose, 20 grams of agar, 1000 mL of water, and make PDA slant culture according to the routine base, inoculate black fungus strains on it, and cultivate at 28°C for 8 days to obtain black fungus female species;

[0044] (2) Erlenmeyer flask liquid fermentation: inoculate the female black fungus seed obtained in step (1) into a 500 ml triangular flask filled with 200 ml of selenium-enriched liquid medium, and inoculate 2 triangular flasks for each female seed, and cultivate it in selenium-enriched liquid medium Basic formula: 20 grams of glucose, 2 grams of yeast extract, 2 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 60 mg of sodium selenite, 1000 ml of water; shaker speed 160r / min, fermented at 23°C for 7 days, get fermentation broth;

[0045] (3) Expanded cultivation: Inocu...

Embodiment 3

[0051] (1) Cultivation of slant parent species: inoculate black fungus strain G6 on PDA slant medium, and prepare PDA slant medium according to: 20 grams of potato, 20 grams of glucose, 20 grams of agar, 1000 mL of water, and make PDA slant culture according to the routine base, inoculate black fungus strains on it, and cultivate at 28°C for 12 days to obtain black fungus female species;

[0052] (2) Erlenmeyer flask liquid fermentation: inoculate the female black fungus seed obtained in step (1) into a 500 ml triangular flask filled with 200 ml of selenium-enriched liquid medium, and inoculate 2 triangular flasks for each female seed, and cultivate it in selenium-enriched liquid medium Basic formula: 20 grams of glucose, 2 grams of yeast extract, 2 grams of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 60 mg of sodium selenite, 1000 ml of water; shaker speed 160r / min, fermented at 28°C for 5 days, get fermentation broth;

[0053] (3) Expanded cultivation: Inoc...

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Abstract

The invention discloses rich-selenium black fungus fermentation slurry. The rich-selenium black fungus fermentation slurry is prepared by adopting the method comprises the following steps that (a) a black fungus strain is cultured into a black fungus mother strain; (b) the black fungus mother strain is subjected to rich-selenium culture to obtain a rich-selenium black fungus mycelium; (c) the rich-selenium black fungus mycelium and water are evenly mixed in proportion, and grinding and ultrasound operation are performed to obtain rich-selenium black fungus mycelium slurry; (d) the black fungus strain is subjected to rich-selenium culture to obtain a black fungus sporocarp, and drying is performed to obtain a dried black fungus sporocarp; (e) the dried black fungus sporocarp is smashed and is evenly mixed with water in proportion, and grinding and ultrasound operation are performed to obtain rich-selenium black fungus sporocarp slurry; (f) the rich-selenium black fungus sporocarp slurry is evenly mixed with the rich-selenium black fungus mycelium slurry in proportion to obtain the rich-selenium black fungus fermentation slurry. The method is simple, easy to operate, convenient, reliable, short in preparation time and low in cost, and the prepared product is stable in character, uniform, good in taste and good in blood fat reducing functionality and is a health-care food easy to popularize in the market, low in cost and high in economic value.

Description

technical field [0001] The invention relates to a method for preparing a fungus health drink, in particular to a selenium-enriched black fungus fermented liquid and its preparation method and application. Background technique [0002] Black fungus, also known as black cabbage, mulberry fungus, this fungus, tree chicken, wood moth, wood antler, is named for its shape like an ear and its dark brown color. Black fungus is a plant of the family Auriculariaceae. Stomach and large intestine meridian, medical research proves that black fungus has natural nutritional and health effects, not only rich in nutrition but also can nourish qi, strengthen the body, nourish the kidney and stomach, etc. Black fungus also has the function of promoting blood circulation, anti-coagulation, anti-thrombosis, and lowering blood fat , Reduce blood viscosity, soften blood vessels and other functions, effectively reduce the occurrence of cardiovascular diseases and other functions. The development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L31/00A23L33/00A01G1/04C05G3/00A23L5/30
CPCA01G18/00A23V2002/00C05B7/00C05G3/00C05C9/00C05D3/00C05F11/00A23V2200/30A23V2250/1626A23V2200/14A23V2200/16A23V2200/3262A23V2250/208A23V2300/14A23V2300/48
Inventor 顾雅君刘建荣张瑞英
Owner HEBEI UNIVERSITY
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