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Quality detecting method for traditional Chinese medicine composition for treating hematuresis

A technology of composition and traditional Chinese medicine, which is applied in the field of quality detection of traditional Chinese medicine composition, can solve the problems that the quality of Chinese medicine composition cannot be well reflected, and achieve good spot expansion effect, optimized TLC identification, and simple method Effect

Active Publication Date: 2016-04-06
云南雷允上理想药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only using the identification of Phellodendron Phellodendri and Imperatae Rhizome as the measurement index in the quality control method of the buccal tablet cannot reflect the quality of the traditional Chinese medicine composition for treating hematuria well

Method used

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  • Quality detecting method for traditional Chinese medicine composition for treating hematuresis
  • Quality detecting method for traditional Chinese medicine composition for treating hematuresis
  • Quality detecting method for traditional Chinese medicine composition for treating hematuresis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] 1 TLC identification of Rhizoma Imperatae

[0075] 1.1 Revision of the identification method of Rhizoma Imperatae TLC

[0076] In the thin-layer chromatography test of Rhizoma Imperatae, use dichloromethane: methanol = 9: 1 as the developing agent, spray with 10% sulfuric acid ethanol solution after development, and heat at 105 ° C until the spots become clear. The results refer to figure 1 . The position of the main spot is high, and the unfolding effect is not good. Use dichloromethane: ethyl acetate = 4: 1 as the developing solvent, see the results figure 2 . The results showed that the thin-layer spots were clear, and the negative control had no interference. Therefore, dichloromethane:ethyl acetate=4:1 was used as the developing solvent to identify the Rhizoma Imperatae by thin-layer chromatography.

[0077] The final identification method of Rhizoma Imperatae TLC is as follows:

[0078]Take 2g of the traditional Chinese medicine composition of this product f...

Embodiment 2

[0085] 2 Establishment of TLC identification method for kidney tea

[0086] 2.1 Take the batch number of the traditional Chinese medicine composition for the treatment of hematuria: 5g each of 20130903, 20130904, and 20131012, add 20ml of water, ultrasonically treat for 25 minutes, centrifuge, take the supernatant, shake and extract 2 times with ethyl acetate, 20ml each time, and combine ethyl acetate The liquid was evaporated to dryness, and the residue was dissolved in 1 ml of ethyl acetate as the test solution. Take another 2g of kidney tea reference medicinal materials, add 40ml of water, boil for 30 minutes, filter, concentrate the filtrate to about 20ml, shake and extract twice with ethyl acetate, 20ml each time, combine the ethyl acetate solutions, evaporate to dryness, and add acetic acid to the residue. 1 ml of ethyl ester was dissolved to prepare a reference medicinal material solution. For thin-layer chromatography test, draw 5-10 μl of the test solution and the re...

Embodiment 3

[0093] 3 Establishment of a method for identification of small thistle by thin layer chromatography

[0094] 3.1 The mongolin in Xiaoji is one of the most active flavonoids in Xiaoji, and it is also the most abundant flavonoid in Xiaoji decoction pieces. It is proposed to establish a thin-layer chromatographic identification method for thistle and montanulin in preparations.

[0095] Take 0.5g of the Chinese medicine composition for the treatment of hematuria, add 10ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 1ml of methanol to the residue to dissolve it, and use it as the test solution. Another 1 g of Xiaoji reference medicinal material was added, 10 ml of methanol was added, sonicated for 30 minutes, filtered, the filtrate was evaporated to dryness, and the residue was dissolved in 1 ml of methanol to prepare a reference medicinal material solution. Then take the reference substance of mongolic acid, add methanol to ma...

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Abstract

The invention relates to a quality detecting method for a traditional Chinese medicine compound preparation, in particular to a quality detecting method for a traditional Chinese medicine composition for treating hematuresis. According to the technical scheme, the quality detecting method for the traditional Chinese medicine composition for treating hematuresis is prepared from cortex phellodendri, lalang grass rhizome, clerodendranthus spicatus and linarin in herba cepbalanoplosis segeti. The invention further provides a method for detecting the content of berberine hydrochloride in cortex phellodendri and the content of rosmarinic acid in clerodendranthus spicatus. High performance liquid chromatography is adopted. According to the method, lalang grass rhizome, cortex phellodendri, clerodendranthus spicatus and herba cepbalanoplosis segeti in the traditional Chinese medicine composition for treating hematuresis are qualitatively detected through thin-layer chromatography, and the method is simple and stable. The high performance liquid chromatography is used for detecting the content of berberine hydrochloride in cortex phellodendri and the content of rosmarinic acid in clerodendranthus spicatus, the method is easy and convenient and high in repeatability and stability, the result is accurate, the method serves as a method for detecting the content of ingredients of the traditional Chinese medicine compound preparation, and the rationality and reliability of the whole quality control method are enhanced.

Description

technical field [0001] The invention relates to a method for measuring the quality of a traditional Chinese medicine compound preparation, in particular to a method for measuring the quality of a traditional Chinese medicine composition for treating hematuria. Background technique [0002] The prescription of the traditional Chinese medicine composition for the treatment of hematuria is a secret recipe passed down by Bo Yubo, a famous old Dai doctor of the Zhongdai Medical Hospital in Jinghong City, Xishuangbanna Prefecture, which has been used for more than 40 years to clinically treat renal edema, hematuria and hematuria. After repeated clinical application, the curative effect is definite, and no adverse reactions and toxic side effects have been found. In 1996, it was voluntarily dedicated to Yunnan Ideal Pharmaceutical Co., Ltd. for development and research, and was approved by the Yunnan Provincial Health Department on April 12, 1999 to be produced in accordance with t...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
CPCG01N30/02G01N30/90G01N2030/027
Inventor 罗实罗红艳
Owner 云南雷允上理想药业有限公司
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