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Serum-free culture medium for culturing virus and preparation method thereof

A technology of serum-free culture medium and culture medium, which is applied in the field of culture medium and its preparation, can solve problems such as unfavorable preparation, complex medium components, and high production costs, and achieve good growth and division, large-scale production, and good quality Effect

Active Publication Date: 2018-04-13
ZHAOQING DAHUANONG BIOLOGIC PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of the medium is complex, which is not conducive to preparation and high production cost

Method used

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  • Serum-free culture medium for culturing virus and preparation method thereof
  • Serum-free culture medium for culturing virus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Serum-free medium for culturing viruses.

[0020] A serum-free culture fluid for cultivating viruses, said serum-free culture fluid for cultivating viruses, including DMEM medium, also includes the following components and their concentrations: insulin 5 mg / L, bovine serum albumin 0.5 mg / mL, trace element 0.5mg / L, yeast extract 0.2g / L, amino acid solution 2mg / L, fibronectin 0.2mg / mL, coenzyme R 0.01mg / L, niacin 0.5 mg / L and vitamin C 0.5 mg / L.

[0021] Wherein, the amino acid solution is composed of the following components in mass percentage: 0.2 parts of glycine, 0.5 parts of isoleucine, 0.2 parts of serine, 0.1 parts of alanine, 0.1 parts of phenylalanine, 0.1 parts of tyrosine, Valine 0.2, Proline 0.1, Cysteine ​​0.5, Leucine 0.2, Tryptophan 0.5, Methionine 0.5, Asparagine 0.05, Glutamine 0.5, Threonine 0.2 part, 0.05 part of aspartic acid, 0.1 part of glutamic acid, 0.5 part of lysine, 0.1 part of arginine, 0.1 part of histidine and 5 parts of distille...

Embodiment 2

[0025] Embodiment 2: Serum-free medium for culturing virus.

[0026] A serum-free culture solution for cultivating viruses, said serum-free culture solution for cultivating viruses, including DMEM medium, is characterized in that it also includes the following components and their concentrations: insulin 25mg / L, bovine serum white Protein 2mg / mL, trace element 1mg / L, yeast extract 1.5g / L, amino acid solution 2.5mg / L, fibronectin 1mg / mL, coenzyme R0.5mg / L, niacin 1mg / L and vitamin C1 mg / L L.

[0027] Wherein, the amino acid solution is composed of the following components in parts by weight: 0.5 parts of glycine, 1 part of isoleucine, 0.6 parts of serine, 1 part of alanine, 0.3 parts of phenylalanine, 1.5 parts of tyrosine, Valine 1 part, Proline 0.2 part, Cysteine ​​0.8 part, Leucine 0.5 part, Tryptophan 1.2 part, Methionine 1.5 part, Asparagine 0.15 part, Glutamine 1.5 part, Threonine 0.9 parts, 1 part of aspartic acid, 0.3 parts of glutamic acid, 0.6 parts of lysine, 0.3 p...

Embodiment 3

[0031] Embodiment 3: Serum-free medium for culturing virus.

[0032] A serum-free culture solution for cultivating viruses, said serum-free culture solution for cultivating viruses, including DMEM medium, is characterized in that it also includes the following components and their concentrations: insulin 45mg / L, bovine serum white Protein 3mg / mL, trace elements 2mg / L, yeast extract 3g / L, amino acid solution 5 mg / L, fibronectin 1.5mg / mL, coenzyme R 0.15mg / L, niacin 2mg / L and vitamin C 2 mg / L.

[0033] Wherein, the amino acid solution is composed of the following components in parts by weight: 1 part of glycine, 2 parts of isoleucine, 1.5 parts of serine, 1.5 parts of alanine, 0.5 parts of phenylalanine, 2 parts of tyrosine, 1.5 parts of valine, 0.5 parts of proline, 2 parts of cysteine, 1 part of leucine, 2 parts of tryptophan, 1.5 parts of methionine, 0.25 parts of asparagine, 2.5 parts of glutamine, threonine 1.5 parts, 2 parts of aspartic acid, 0.5 parts of glutamic acid,...

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Abstract

The invention relates to a serum-free medium for culturing viruses and a preparation method thereof. The serum-free medium comprises a DMEM (Dulbecco Modified Eagle Medium) culture medium, 5-45mg / L of insulin, 0.5-3mg / mL of bovine serum albumin, 0.1-2mg / L of trace elements, 0.2-3g / L of yeast extract, 2-5mg / L of amino acid solution, 0.2-1.5mg / mL of fibronectin, 0.01-0.15mg / L of coenzyme R, 0.5-2mg / L of nicotinic acid and 0.5-2mg / L of vitamin C. The serum-free medium for culturing viruses is simple in preparation method, is lower in vaccine production cost, and is easy to operate. Moreover, viruses cultured through the serum-free medium have the advantages of high valence and high quality.

Description

technical field [0001] The invention relates to a culture solution and a preparation method thereof, in particular to a serum-free culture solution for cultivating viruses and a preparation method thereof. Background technique [0002] Since 1996, mammalian cell culture has been developed to allow free scale-up and industrial production. Many biologically active substances, vaccines, and carriers, such as monoclonal antibodies, pharmaceutical proteins, and PRRS, can all be obtained through large-scale culture of animal cells. The production of biological products through cell culture can not only improve the quality of biological products, but also promote the development of cell culture technology, protein expression and purification technology, and virus culture technology. [0003] At present, many veterinary vaccines need to be produced by cell culture, and serum is required in the production process. The composition of serum culture is not clear; the storage period is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/07
CPCC12N5/0037C12N2500/05C12N2500/30C12N2500/32C12N2500/38C12N2500/74C12N2500/90C12N2501/33C12N2501/998
Inventor 陈瑞爱徐家华唐兆新张文炎高艳
Owner ZHAOQING DAHUANONG BIOLOGIC PHARMA
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