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Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng black spot

A technology of TRXY-46 and endophytic fungi, applied in the biological field, can solve problems such as threats to health, pollution, and production reduction of Panax notoginseng, and achieve strong inhibitory effects and broad application prospects

Active Publication Date: 2016-04-06
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional authentic production area of ​​Sanqi in Guangxi, due to the low altitude and high temperature and humidity weather conditions, this disease often occurs at the same time, resulting in a large amount of production reduction or even failure of Sanqi, and brought huge economic losses to the growers.
In order to control this fungal disease, a large amount of chemical pesticides are used, resulting in a large amount of pesticide residues in Panax notoginseng products, seriously polluting the environment, and greatly threatening people's health

Method used

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  • Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng black spot
  • Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng black spot
  • Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng black spot

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Experimental program
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Effect test

Embodiment 1

[0024] Isolation, screening and identification of strains

[0025] 1. Isolation of strains

[0026] Test material: wild Vietnamese pagoda tree collected from the limestone area of ​​Tiandeng County, Guangxi.

[0027] Tested strains: provided by Institute of Plant Pathology, Guangxi University.

[0028] Medium: 1000ml potato dextrose agar medium (PDA medium), PDA medium contains 300g of potatoes, 20g of glucose, 20g of agar and 0.1g of chloramphenicol, and the pH of the medium is 6.0±0.2.

[0029] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (availa...

Embodiment 2

[0079] The Inhibitory Effect of the Metabolites of the Strain on Pseudomonas notoginseng

[0080] 1. Fermentation culture of bacteria and extraction of fermentation products

[0081] (1) Inoculate the endophytic fungus TRXY-46 of Sophora vietnamese on a flat plate of potato dextrose agar medium (PDA plate), culture it at 28°C for 20 days, cut the obtained culture material into pieces and transfer it to a sterile In a 2 liter Erlenmeyer flask of solid medium (containing 400g of potatoes, 20g of dextrose and 20g of sucrose), ferment for 60 days at 28°C;

[0082] (2) After the fermentation is completed, soak the fermented product with 2 times the amount of methanol of the fermented product and sonicate it for 40 minutes, then filter it with gauze, and get the filtrate;

[0083] (3) Repeat step (2) twice, combine the two filtrates and concentrate under reduced pressure to form an extract to obtain the crude methanol extract of the endophytic fungus TRXY-46 fermentation product, w...

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Abstract

The invention discloses a sophora tonkinensis endophytic fungus TRXY-46. The sophora tonkinensis endophytic fungus TRXY-46 is classified and named as Rhexocercosporidium sp.TRXY-46, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 03 December 2014, and the depository number is CGMCC No.10108. According to the sophora tonkinensis endophytic fungus TRXY-46 provided by the invention, a strain of endophytic fungus Rhexocercosporidium sp.TRXY-46 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Rhexocercosporidium sp.TRXY-46 has a strong inhibiting effect on panax notoginseng alternaria panax whetzel, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a Vietnamese Sophora japonica endophyte TRXY-46 in the prevention and treatment of Panax notoginseng black spot. Background technique [0002] The prevention and control of plant diseases in modern agriculture is overly dependent on the use of chemical pesticides. The application of a large number of chemical pesticides not only has long-term damage to the ecological environment, but also causes the quality of agricultural products to decline, pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, and are harmful to humans and animals. Many questions. It is of great significance to find safer and more effective methods of disease control, and the use of biological methods to prevent and control plant diseases can effectively solve the above problems. [0003] Panax notoginseng F.H.chen, also known as Tianqi, Jinbuhuan, etc., has signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 姚裕群黄荣韶吴炫柯李良波
Owner GUANGXI UNIV
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