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A kind of artificial cornea and preparation method thereof

A technology of artificial cornea and corneal stroma, which is applied in the field of medical materials, can solve problems such as unstoppable disease, and achieve the effects of improving mechanical strength, improving biomechanical properties, and improving biomechanical strength and stability

Active Publication Date: 2018-05-29
GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above, the photooxidative collagen cross-linking method is mainly used clinically as a therapy for treating diseases. It is relatively safe as a therapy, but due to the influence of some patients with advanced disease or postoperative complications, the disease has not been prevented, or There are certain defects due to the limitation of treatment depth

Method used

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  • A kind of artificial cornea and preparation method thereof
  • A kind of artificial cornea and preparation method thereof
  • A kind of artificial cornea and preparation method thereof

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preparation example Construction

[0034]The preparation method of the artificial cornea of ​​the present invention mainly uses the animal acellular corneal stroma as the decellularized scaffold, wherein a photosensitizer that can absorb light and transmit the absorbed light energy to the reactant and has no toxicity or low toxicity to humans or animals is used. photooxidative crosslinking. Under the condition of light irradiation (visible light or ultraviolet light irradiation), the photosensitizer is excited to the triplet state and releases the active oxygen group dominated by singlet oxygen, and the active oxygen is chemically active and extremely unstable, and can be combined with various This kind of molecular reaction induces a chemical cross-linking reaction (type II photochemical reaction) between the molecular groups of collagen fibers (such as amino groups), forming new molecular chains within or between collagen fibers, improving the mechanical strength of collagen fibers. The photosensitizers are p...

Embodiment 1

[0038] A kind of preparation method of artificial cornea, the steps that it comprises are as follows:

[0039] (1) Soak the animal decellularized corneal stroma in a photosensitizer solution with a concentration of 0.05 mg / ml for 30 minutes;

[0040] (2) The immersed animal acellular corneal stroma was taken out and placed in a petri dish, and irradiated with 110 nm wavelength ultraviolet light for 40 minutes; when the ultraviolet light was irradiated for 20 minutes, the surfaces were changed, and then continued to irradiate, and the power density of the irradiated ultraviolet light was 3 mw / cm 2 ;

[0041] (3) Soak with PBS solution for 0.6h, then shake for 0.6h;

[0042] (4) Change the PBS solution to soak for 0.6h, and then shake for 0.6h.

Embodiment 2

[0044] A kind of preparation method of artificial cornea, the steps that it comprises are as follows:

[0045] (1) Soak the animal decellularized corneal stroma in a photosensitizer solution with a concentration of 4.5 mg / ml for 55 minutes;

[0046] (2) taking out the immersed animal acellular corneal stroma and placing it in a petri dish, and irradiating it with 380nm wavelength ultraviolet light for 56min; changing the surface when irradiating for 28min, and then continuing to irradiate; in the step (2), the power of the irradiation Density at 5800mw / cm 2 ;

[0047] (3) soak in PBS solution for 4h, then shake for 2h;

[0048] (4) Replace the PBS solution, soak for another 4 hours, and then shake for 2 hours.

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Abstract

The invention discloses an artificial cornea. Animal decellularized corneal stroma is first soaked in a photosensitizer, and then uniformly cross-linked under ultraviolet light irradiation. The steps of a method for photooxidative collagen cross-linking are as follows: (1) Soak animal decellularized corneal stroma in 0.01-5mg / ml photosensitizer solution for 1-60min; (2) Take out the soaked animal decellularized corneal stroma Put it in a petri dish, irradiate with 100-400nm wavelength ultraviolet light for 1-60min; (3) Soak in PBS solution for 0.5-4h, then shake for 0.5-2h. The beneficial effects of the present invention are: the biomechanical properties such as the mechanical strength of the decellularized corneal stroma can be effectively improved; because the biomechanical properties mainly depend on the composition of collagen fibers, collagen fiber bundles and their spatial structures, therefore, through light radiation Irradiation / photosensitizer cross-linking can improve the biomechanical properties and stability of animal decellularized corneal stroma.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to an artificial cornea and a preparation method thereof. Background technique [0002] The basic principle of photochemical collagen crosslinking is photooxidation. According to the unique absorption spectrum of the photosensitizer, within its maximum absorption wavelength range, after being irradiated with a certain wavelength, the photosensitizer absorbs photon energy, excited to form a triplet state, has a strong oxidizing ability, and produces superoxide radicals or singlet states. Oxygen and oxygen free radicals have the activity of directly modifying collagen molecules, causing the formation of covalent bonds within or between amino acid residues in collagen, increasing the density of covalent bonds between collagen fibers, thereby improving the biomechanical properties of collagen (type II photochemical properties). reaction). With the continuous research on the photooxida...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
Inventor 官习鹏易静楠
Owner GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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