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Ceruloplasmin detection kit

A technology for detecting kits and ceruloplasmin, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of low sensitivity, limited popularization and application, poor stability of ceruloplasmin detection reagents, etc., and achieves improved Analytical Sensitivity, Enhanced Stability Effects

Inactive Publication Date: 2016-02-17
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, common ceruloplasmin detection reagents have poor stability and low sensitivity, which limits their clinical application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A conventional ceruloplasmin detection kit comprises reagent R1, reagent R2 and calibrator.

[0027] Wherein the reagent R1 consists of:

[0028] pH8.0 Tris buffer 50mmol / L

[0029] Sodium azide 0.1%

[0030] Polyethylene glycol 40.0g / L

[0031] Reagent R2 consists of:

[0032] pH8.0 Tris buffer 50mmol / L

[0033] Goat anti-human APOE antibody 30ml / L

[0034] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0035] Add 5 μl of physiological saline, sample or calibrator, then add 225 μl of R1 reagent for pre-incubation for 5 minutes, then add 75 μl of reagent R2, then read the absorbance A1, after 5 minutes of reaction, read the absorbance A2, and calculate ΔA.

[0036] The calibrator used in this example is the CP calibrator produced by Shanghai Yuansheng Biotechnology Co., Ltd.

Embodiment 2

[0038] A ceruloplasmin detection kit, which comprises reagent R1, reagent R2, and calibrator.

[0039] Wherein the reagent R1 consists of:

[0040]pH8.0 Tris buffer 50mmol / L

[0041] Sodium azide 0.1%

[0042] Polyethylene glycol 40.0g / L

[0043] Silica coated magnetic nanoparticles 0.1%

[0044] Reagent R2 consists of:

[0045] pH8.0 Tris buffer 50mmol / L

[0046] Goat anti-human APOE antibody 30ml / L

[0047] Bovine serum albumin 20g / L

[0048] Kathon-CG0.05%

[0049] Concrete determination method is with embodiment 1.

Embodiment 3

[0051] A ceruloplasmin detection kit, which comprises reagent R1, reagent R2, and calibrator.

[0052] Wherein the reagent R1 consists of:

[0053] pH8.0 Tris buffer 50mmol / L

[0054] Sodium azide 0.1%

[0055] Polyethylene glycol 40.0g / L

[0056] Silica coated magnetic nanoparticles 1%

[0057] Reagent R2 consists of:

[0058] pH8.0 Tris buffer 50mmol / L

[0059] Goat anti-human APOE antibody 30ml / L

[0060] Bovine serum albumin 30g / L

[0061] Kathon-CG0.05%

[0062] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses a ceruloplasmin detection kit, and belongs to the technical field of clinic in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2 and a calibration sample. By adding a certain amount of a silicon-dioxide-coated magnetic nano-particle into the reagent R1 and adding a certain amount of bovine serum albumin and Kathon-CG into the reagent R2, the kit stability and analysis sensitivity are effectively improved, the linearity scope is relatively good, the reagent accurate is high, and further popularization usage of the kit in the market is facilitated.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a ceruloplasmin detection kit. Background technique [0002] Ceruloplasmin (CP), also known as copper oxidase, is a copper-containing α2 glycoprotein with a molecular weight of about 120,000-160,000 and is not easy to purify. It is currently known as a single-chain polypeptide, each molecule contains 6-7 copper atoms, it is blue due to the copper content, contains about 10% sugar, and the terminal sialic acid is connected to the polypeptide chain, which has genetic polymorphism. Its role is to regulate the distribution of copper in various parts of the body, to synthesize copper-containing enzyme proteins, to act as an antioxidant, and to have oxidase activity. It is generally believed that ceruloplasmin is synthesized by the liver, part of it is excreted by the biliary tract, and the content in urine is very small. Determination of ceruloplasmin ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/68G01N33/54346
Inventor 范刚董雯李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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