Preparation method for extracting water-soluble polyose from black pine needles according to decompression inner-boiling method

Abstract

The invention discloses a preparation method for extracting water-soluble polyose from black pine needles according to a decompression inner-boiling method. The preparation method comprises the following steps: putting the black pine needles in a constant-temperature drying cabinet for 24 hours' drying at 40 DEG C, milling the dried black pine needles by a miller, and sieving with a 80-mesh sieve; weighing 1 g of the obtained black pine needle powder, putting the weighed black pine needle powder in a glass device, and adding 2 ml of ethanol for 30 minutes' uniform wetting, so as to ensure that ethanol permeates the black pine needle powder; carrying out vacuum suction to reduce the gauge pressure to -0.080 MPa, carrying out decompression suction till slightly boiled bubbles are generated on the mixture surface, and carrying out filtration; extracting a solution by utilizing hot water of which the volume is 20 times of that of the solution and the temperature is 85 DEG C for 6 minutes; selecting a small amount of the extracted solution for dilution, and measuring the polyose content according to a phenol-sulfuric acid method; precipitating the extracted solution by utilizing a 4% ethanol solution, carrying out still standing for a night, carrying out centrifugation, and then taking out the precipitate for drying in the drying box; preparing the dried solution into a polyose product. Through adoption of the technical scheme, the leaching amount of polyose is improved; the decompression inner-boiling method is low in production cost and simple in process; the preparation method has the advantages that the effective components of polyose can be quickly extracted and separated in an environment-friendly manner.

Description

technical field [0001] The invention belongs to the technical field of extracting plant active ingredients, and in particular relates to a preparation method for extracting water-soluble polysaccharides from black pine needles by internal boiling under reduced pressure. Background technique [0002] Pine needles are the leaves of Pinaceae Pinus plants, and one of the main useless by-products left after pine trees are harvested. It is a fast regeneration speed, harvestable throughout the year, widely distributed, large natural accumulation, and sustainable use. Natural renewable resources. "According to the "Compendium of Materia Medica" records: "Pine and cypress are the length of all trees", taking it "makes people not old, lighten the body and nourish Qi, not hungry and prolong life". Modern research shows that pine needles have calming, analgesic, anti-inflammatory, and anti-aging properties , lowering blood fat, lowering cholesterol and so on. [0003] At present, in t...

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Problems solved by technology

[0004] In order to overcome the problems of long extraction time and low yield of polysaccharides in the existing extraction technology of pine needle polysaccharides, and the problems of severe decomposition of polysaccharides in ultrasonic and microwave-assisted extraction methods, which affect product quality, the invention provides low production cost and simple process. It can realize the rapid and green extraction of active ingredients of pine needle polysaccharides, and the increase of the green extraction temperature begins to reduce the yield. When it exceeds 90°C, the temperature may be too high to destroy the polysaccharide structure and reduce the yield of polysaccharides. Therefore, the selected temperature is 90°C