A multi-index detection microfluidic chip for quantitative shunting

Abstract

The invention discloses a multi-index detection micro-fluidic chip capable of quantitatively shunting and relates to the field of medical detection of micro-fluidic chips. The multi-index detection micro-fluidic chip comprises a substrate and a cover piece, wherein the substrate and the cover piece are bonded to form a sheet-shaped chip; a sample inlet is formed in the cover piece and the substrate is provided with a plurality of grades of shunting branch circuits; each shunting branch circuit is communicated with one end of a corresponding parallel reaction cavity; the sizes of the reaction cavities are the same; the other ends of the shunting branch circuits are communicated with a plurality of buffering tanks through liquid buffering channels; the buffering tanks are connected in series; the sizes of the buffering tanks are gradually reduced along a liquid flowing direction; the tail ends of the liquid buffering channels are communicated with an air outlet formed in the cover piece on the upper layer. When samples are added, by gradually reducing the negative-pressure effect of the buffering tanks, a sample amount in each parallel reaction cavity is accurately controlled and errors between the sample amounts participating in a reaction in each index are reduced. The chip has a simple structure and can be flexibly added according to the requirements on the parallel reaction cavities, so that a multi-index detection reaction is realized and the sample amounts of the reaction cavities are the same; the reaction precision is greatly improved and a result is accurate and reliable.

Description

technical field [0001] The invention relates to a liquid splitting device, in particular to a splitting device which can be arranged on a microfluidic chip and can realize accurate quantification of sample volume in a reaction chamber in multi-index detection. Background technique [0002] The size of the microchannel in the microfluidic microfluidic chip is in a micro-nano structure. Chip materials such as glass, silicon wafers, and polymer materials are mostly hydrophobic materials, and the roughness of the surface of the microchannel is also different. These factors The micro-size effect, surface effect and wall slip effect are caused, and these effects will affect the resistance of the microfluidic flow. During the processing of microchannels, there are certain differences in surface characteristics such as size and roughness among the channels, which makes the fluid flow rate of microfluids in microchannels uneven, and then when multi-channel shunting is performed, the ...

AI Technical Summary

Problems solved by technology

Although the chip can inject enough samples into the reaction cells of the same size connected in series and then use micro-valve isolation to realize that each reaction cell is filled with the same amount of sample, but the interference between the reaction cells caused by the continuous flow of the sample causes The amount of samples participating in the reaction in the reaction pools at different positions will cause large errors, which will also cause doping and pollution between the reactions, which is not conducive to the accurate analysis of the reaction results.

[0005] Invention patent US20150093760 discloses a multi-channel structure that can divide samples into multiple detection reaction chambers. It is equipped with a sample inlet connected to multiple fluid channels, and each fluid channel is connected to a same-sized chamber for index detection reactions to occur. Circular reaction chamber, the reaction chamber is fixed with reagents for index detection. In the structure of the invention, the distances between the multiple liquid channels connected to the sample inlet and the sample inlet are different, and the sample is affected by the microchannel structure during the flow process. Due to the influence of size effect and wall slip effect, each reaction chamber cannot be filled with samples at the same time, resulting in different amounts of samples participating in the reaction in each reaction chamber during detection, which affects the accurate analysis of detection results. It is necessary to ensure that each reaction chamber If the inside is full of samples, the total sample volume must be controlled to achieve excess and sufficient injection time, which is not conducive to efficient reaction and is likely to cause sample waste and pollution

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