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Method for urine metabonomics detection of fatigue based on nuclear magnetic resonance

A technology of nuclear magnetic resonance and metabolomics, applied in the field of testing, can solve problems such as errors and incomplete results, and achieve simple steps, high accuracy, and good repeatability

Inactive Publication Date: 2016-01-06
SHANGHAI UNIV OF SPORT
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Problems solved by technology

, this research model has become a classic paradigm, which promotes the progress of science to a certain extent, but its shortcomings are very obvious. indicators to verify
More importantly, since organisms are a complex system that is dynamic and comprehensively regulated by multiple factors, different components may antagonize and induce each other. Or the degree of influence of external factors, the results obtained are often not comprehensive, and may even contain errors

Method used

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Embodiment 1

[0028] This embodiment relates to a method for detecting fatigue based on NMR-based urine metabolomics, the method comprising the following steps:

[0029] Step 1, perform exercise intervention on the detected body (exhaustive exercise in which anaerobic glycolysis is the main energy supply);

[0030] Step 2, sample collection after 30 minutes of sustained release;

[0031] Step 3, after processing the collected sample, use a nuclear magnetic resonance instrument (600MHzbrokerAvanceIIIHD) to scan the sample comprehensively to obtain a nuclear magnetic resonance spectrum;

[0032] Step 4, using PCA, PLS-DA and OPLS-DA scatter score plots to analyze differential metabolites;

[0033] Step 5, analyze the differential metabolites by means of metabolomics method, and analyze the metabolic pathways of the differential metabolites in the body through the score map;

[0034] In step six, whether fatigue occurs can be judged by the increase or decrease of the content of trimethylamin...

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Abstract

The invention provides a method for urine metabonomics detection of fatigue based on nuclear magnetic resonance. The method includes the steps: step one, carrying out motion intervention on a detected body; step 2, after a slow-release time, collecting a sample; step 3, after treating the collected sample, carrying out comprehensive scanning on the sample by a nuclear magnetic resonance instrument, to obtain a nuclear magnetic resonance atlas; step 4, dispersing by using PCA, PLS-DA and OPLS-DA to obtain a detail drawing analysis difference metabolic substance; step 5, through the metabonomics method, analyzing the detail drawing analysis difference metabolic substance, to obtain a metabolic pathway of the sub char analysis difference metabolic substance in a body; and step 6, according to rising or falling of the content of N-trimethylamine oxide (TMAO) in the sample, determining whether fatigue is generated. The sample is subjected to panoramic scanning by the nuclear magnetic resonance instrument, and the metabolic substance is obtained; through analysis of the metabolic substance, the body physiological state can be comprehensively and systematically reflected, the steps are simple, the accuracy is high, and the repeatability is good.

Description

technical field [0001] The invention relates to a test method, in particular to a method for detecting fatigue based on nuclear magnetic resonance-based urine metabolomics. Background technique [0002] Traditional experimental research on exercise fatigue generally evaluates the state of the body by measuring and comparing the changes of one or a group of indicators before and after exercise. According to some preset hypotheses, researchers select observation indicators in advance, conduct experiments to observe the changes of these indicators, and then negate or affirm the preset hypotheses through statistics and analysis of experimental data. , this research model has become a classic paradigm, which promotes the progress of science to a certain extent, but its shortcomings are very obvious. indicators to verify. More importantly, since organisms are a complex system that is dynamic and comprehensively regulated by multiple factors, different components may antagonize a...

Claims

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Application Information

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IPC IPC(8): G01N24/08
Inventor 马海峰
Owner SHANGHAI UNIV OF SPORT
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