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Separation method of soluble boron in plants

A separation method and soluble technology are applied in the field of separation of soluble boron in plants to achieve the effects of strong operability and simple operation steps.

Inactive Publication Date: 2015-12-30
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between boron content, existing form, plant body fluid pH value, and boron isotope composition. The problem that needs to be solved urgently is the separation of soluble boron in plants, and then study its existing form and body fluid pH value, boron isotope composition. Correlation Between Changes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0023] (1) The plant organ samples were repeatedly washed with tap water and deionized water 5 times each, dried in a drying oven at 40°C, crushed with a plant grinder, and passed through a 60-mesh analytical sieve.

[0024] (2) Discard the piston part of the medical 2.5mL disposable syringe, remove the needle sheath, pull out and discard the needle stem, heat the thin end of the needle plug, flatten it while it is hot, and use it as a syringe stopper Put a solid-phase extraction sieve plate (Restek brand, product number 26017) with a diameter of 9.0 mm and a pore size of 20 μm in the syringe barrel, so that the sieve plate is placed in the syringe barrel close to the nipple, and the plane of the sieve plate is perpendicular to the barrel; Accurately weigh 0.2g of plant samples, place them in a syringe barrel with a sieve plate and a plug, add 2mL of deionized water into the barrel, and place the syringe barrel in an ultrasonic oscillator after the water and plant samples are c...

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Abstract

The invention discloses a separation method of soluble boron in plants. The separation method comprises the following steps: (1) washing, drying, crushing and sieving plant samples; (2) weighing a certain amount of powder sample and putting the powder sample into a needle cylinder with a sieve plate and an end cap; adding water to immerse the sample and ultrasonically vibrating; (3) taking off the end cap of the needle cylinder of an injection syringe and putting the needle cylinder of the injection syringe into a screw-cap centrifugal pipe; centrifuging, separating and collecting filtrates; and (4) repeating the step (2) of adding the water and vibrating, and the step (3), totally separating for 5-10 times, and combining all the filtrates so as to completely separate the soluble boron in the plant samples. The invention establishes the separation method of the soluble boron in the plants; the method can be used for separating the soluble boron in plant sample fluid from non-soluble boron in tissue residues; the method has the characteristics of simplicity and reliability, and wide application prospect, and provides new technical supports for researches in the fields including plant boron nutrition, physiology and biochemistry of the boron, biogeochemical cycles of the boron and the like.

Description

technical field [0001] The invention relates to a method capable of completely separating soluble boron in plants. Background technique [0002] Boron is one of the micronutrient elements necessary for plants, and plays a very important role in plant growth and development, the establishment and development of plant reproductive organs, and plant photosynthesis. The rapid development of analytical testing has led to the development of boron determination methods. The current methods of boron determination include: spectrophotometry (formimine H acid method, curcumin method and beryllium reagent, etc.), fluorescence photometry, atomic absorption spectrometry (AAS), plasma emission spectrometry (ICP-OES), plasma mass spectrometry (ICP-MS), etc. These methods have improved the measurement accuracy of boron in plants and made the measurement results more reliable. At present, in the analysis and testing of boron in plants, various techniques (acid dissolution method, dry ashing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 孙爱德
Owner LINYI UNIVERSITY
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