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Neural restoration material combined with acellular nerve application

A nerve repair and decellularization technology, applied in the field of nerve repair materials, can solve problems such as lack of nerve repair materials, and achieve the effects of ensuring high efficiency, facilitating directional growth, and protecting activity

Active Publication Date: 2015-12-23
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported nerve repair strategies lack an ideal nerve repair material, which can provide the best physical, chemical and biological microenvironment for nerve regeneration, achieve the dual purpose of inhibiting neuroma formation and promoting nerve repair, and can be maintained for a long time Favorable microenvironment at the site of nerve injury, maintaining the activity of various nutritional proteins

Method used

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  • Neural restoration material combined with acellular nerve application
  • Neural restoration material combined with acellular nerve application
  • Neural restoration material combined with acellular nerve application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Animal Modeling and Evaluation of Nerve Injury

[0031] a. Rat sciatic nerve injury model

[0032] Detoxified adult SD rats, male or female, were anesthetized by intraperitoneal injection of 1% sodium pentobarbital at a dose of 40 mg / kg rat body weight. A longitudinal incision was made at the posterior part of the right thigh, the skin was cut open, and the muscles were separated. The right sciatic nerve was exposed through the space of the posterolateral muscle, and the sciatic nerve was cut off with a double-sided blade at 8 mm from the lower edge of the piriformis muscle.

[0033] b. Canine sciatic nerve injury model

[0034] A young Beagle dog was selected and anesthetized by intraperitoneal injection of sodium pentobarbital (30 mg / kg). A longitudinal incision was made at the posterior part of the right thigh, the skin was cut, and the muscle was separated to expose the right sciatic nerve. The sciatic nerve was cut off with a double-sided blade.

[003...

Embodiment 2

[0039] Example 2 Preparation of decellularized biomaterials

[0040] a. Canine sciatic nerve homogenate

[0041] Young Beagle dogs were anesthetized by intraperitoneal injection of pentobarbital sodium (30mg / kg), separated the right sciatic nerve, removed the muscle and fat tissue around the nerve, and rinsed with sterile distilled water 2 to 3 times, 3 to 5 minutes each time; Add it to the phosphate buffer solution containing protease inhibitors 0.005-0.1% PMSF and 0.01-1% EDTA, crush it into small pieces by a pulverizer; use sodium citrate buffer solution (pH=3-5) to expand the tissue, and keep Shake continuously for 24-72 hours; wash 2-3 times with sterile distilled water, 3-5 minutes each time; add 0.5-2% TritonX-100 in 10mM / L Tris-HCL buffer (pH=7.5) at 4°C , the buffer also contains protease inhibitors 0.005-0.1% PMSF and 0.01-1% EDTA, and continuously shakes for 24-72 hours to remove cell components; after centrifugation, wash with PBS repeatedly; digest with DNase and...

Embodiment 3

[0048] The preparation of embodiment 3 restorative material

[0049] a. Collagen Scaffold

[0050] Prepare a collagen solution with a concentration of 25 mg / ml, pour it into a mold, freeze at -15°C for 80 minutes, and then put it into a freeze dryer for 25 minutes at -60°C in a vacuum; take out a columnar collagen material with a length of 1.2 cm and a diameter of 1.2 mm.

[0051] Slowly stretch the above-mentioned collagen material to 2.5 times the original length at a speed of 1.4 mm per second, and then freeze it at -15°C for 80 minutes in the stretched state, and then put it in a vacuum at -80°C. The second freeze-drying was performed for 1.5 hours to obtain a linear scaffold containing ordered micro-pipes inside, that is, a collagen scaffold.

[0052] b. Silk protein sponge

[0053] ①Put 100g of silk raw silk into 1250ml of 0.05% sodium carbonate aqueous solution, raise the temperature to 100°C, boil for 0.5h, repeat the cooking 3 times, remove all the sericin on the su...

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Abstract

The invention aims to provide a neural restoration material combined with the acellular nerve application. The neural restoration material is prepared from nerves of an acellular allogene or acellular heterogeneous living organism, a restoration material, a microtubulin inhibitor, neurotrophic factors and stem cells. The neural restoration material disclosed by the invention has good biocompatibility, can provide nutrition supplies needed by nerve regeneration and restoration, is capable of maintaining an optimal physicochemical and biology microenvironment for nerve regeneration for a long time and has dual functions of preventing nerve tumor formation and promoting defect nerve restoration.

Description

technical field [0001] The invention relates to providing a nerve repair material that maintains a favorable microenvironment of a nerve injury site for a long time, prevents the formation of neuromas, and promotes the regeneration of defective nerves. Background technique [0002] With the development of modern society, the number of people with nerve injuries caused by accident injuries, wars, earthquakes, tumor surgery, etc. is increasing year by year. In the annual new trauma cases in the world, nerve injury cases account for about 1.5%-4.0%. In my country alone, there are 600,000 to 900,000 new cases of central nervous or peripheral nerve injury each year, and nerve transplantation is needed to repair the nerve. If the treatment is not done properly, it will cause permanent disability. The repair and reconstruction of nerve defects is a major problem in trauma surgery and microsurgery. [0003] Regeneration after nervous system injury is limited by inhibitory influence...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/22A61L27/50A61L27/54A61L27/58
Inventor 赵应征鲁翠涛肖健林丽张宏宇张翼杨伟傅红兴徐荷林
Owner WENZHOU MEDICAL UNIV
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