A kind of method utilizing biotransformation method to prepare γ-aminobutyric acid

A technology of aminobutyric acid and biotransformation is applied in the field of preparation of γ-aminobutyric acid, and can solve the problems of large dosage, low output of γ-aminobutyric acid, large heat release during fermentation and the like

Active Publication Date: 2018-02-23
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Whether the above-mentioned published patents are purified strains obtained through separation and mutagenesis or engineered strains; whether they are fermented by a single strain or mixed strains, the method for producing γ-aminobutyric acid by fermentation still has the following problems: Problems in two aspects: (1) The fermentation period is long, usually tens to hundreds of hours; (2) The heat released during the fermentation process is large, the amount of cooling circulating water is large, and the energy consumption is high; (3) The composition of the fermentation liquid is relatively complicated, and the extraction The cost is high; (4) The output of waste residue is relatively large, it is difficult to filter bacteria, and the amount of activated carbon, perlite and other substances is large, and the by-products bring processing pressure to the enterprise
The patent disclosed above still has defects such as low yield of GABA, long conversion time, low conversion rate, etc., and the effect is not ideal

Method used

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Examples

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Embodiment 1

[0033] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:

[0034] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 once, inoculate 1 ring in the seed medium, and inoculate it at 5% (V / V) after static culture at 30°C for 12 hours Put it into the fermentation medium, and culture it statically at 30°C for 16 hours to obtain the fermentation liquid;

[0035] The seed medium consists of: 15g of corn saccharification solution, 5g of peptone, 5g of yeast extract powder, 1g of ammonium citrate, 0.3g of magnesium sulfate, 0.1g of manganese sulfate, 1g of sodium acetate, 1g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;

[0036] The fermentation medium consists of: 20 g of corn saccharification liquid, 10 g of yeast extract powder, 10 g of corn steep liquor, 1 g of sodium acetate, 1 g of potassium phosphate, 0.2 g of magnesium sulfate, 0.1 g of ammonium sulfate, 1000 mL of disti...

Embodiment 2

[0041] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:

[0042] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 twice, inoculate 2 rings in the seed medium, and culture at 35°C for 12 hours, then inoculate with an inoculation amount of 20% (V / V) Put it into the fermentation medium, and culture it statically at 35°C for 32 hours to obtain the fermentation broth;

[0043] The seed medium consists of: 30g of corn saccharification solution, 15g of peptone, 10g of yeast extract powder, 5g of ammonium citrate, 1.0g of magnesium sulfate, 0.5g of manganese sulfate, 4g of sodium acetate, 4g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;

[0044] The fermentation medium is composed of: 30 g of corn saccharification liquid, 20 g of yeast extract powder, 30 g of corn steep liquor, 4 g of sodium acetate, 4 g of potassium phosphate, 0.8 g of magnesium sulfate, 0.5 g of ammonium s...

Embodiment 3

[0049] A method for preparing gamma-aminobutyric acid by biotransformation, comprising the steps of:

[0050] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 twice, inoculate 2 rings in the seed medium, and incubate at 33°C for 12 hours, then inoculate with an inoculum of 10% (V / V) Put it into the fermentation medium, and culture it statically at 33°C for 48 hours to obtain the fermentation broth;

[0051] The seed medium consists of: 25g of corn saccharification solution, 10g of peptone, 8g of yeast extract powder, 3g of ammonium citrate, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 2g of sodium acetate, 3g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;

[0052] The fermentation medium is composed of: 25 g of corn saccharification liquid, 15 g of yeast extract powder, 20 g of corn steep liquor, 3 g of sodium acetate, 2 g of potassium phosphate, 0.3 g of magnesium sulfate, 0.2 g of ammonium sulfate, 1000 m...

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Abstract

The invention discloses a method for preparing gamma-aminobutyric acid by using a biotransformation method, and belongs to the technical field of gamma-aminobutyric acid preparation. Lactobacillus brevis CGMCC NO.3414 was used as the starting strain, after multiplication culture and cell collection, the bacteria were resuspended in the buffer solution of the scientific compound L-sodium glutamate and glutamic acid mixture, and the cells produced by the strain were added Na2B4O7·10H2O or MgSO4 with strong activating effect on internal enzymes can maximize the activity of intracellular enzymes and catalyze the conversion of L-sodium glutamate and glutamic acid mixture into γ-aminobutyric acid. The γ-aminobutyric acid in the conversion solution ‑GABA content is 45.74‑202.18g / L, substrate molar conversion rate is 95.92‑99.75%, and the bacteria can be reused 8 times. The method not only improves product quality and output, but also protects the environment, has better economic value and social benefit, can be produced in a large scale, and is beneficial to industrial popularization.

Description

technical field [0001] The invention relates to the preparation of gamma-aminobutyric acid, in particular to a method for preparing gamma-aminobutyric acid by a biotransformation method. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA) is a natural non-protein amino acid widely present in animals and plants. In mammals, γ-aminobutyric acid, as an important inhibitory neurotransmitter in the brain and spinal cord, is produced by glutamate (Glutamic acid, Glu) in glutamate decarboxylase (Glutamate decarboxylase, GAD, EC4.1.1.15 ) under the action of catalytic decarboxylation transformation, and participate in a variety of metabolic activities, has high physiological activity, has the effects of lowering blood pressure, regulating arrhythmia, improving sleep, anti-anxiety, improving lipid metabolism, and preventing arteriosclerosis. By more and more scientific workers' attention. In Japan, research on this type of GABA-rich functional food has mad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12R1/24
Inventor 高强高年发石秀峰常传友朱燕
Owner TIANJIN UNIV OF SCI & TECH
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