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Method for producing raw oil of biodiesel

A technology for biodiesel and a production method, which is applied in the fields of biotechnology and renewable energy, can solve the problems of low output, complex process, long cycle of biodiesel feedstock oil, etc., and achieves the effects of low production cost, high efficiency, and easy industrial production.

Inactive Publication Date: 2015-11-25
WUHAN HUASHITE IND BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a production method of biodiesel raw material oil, which overcomes the problem that Schizochytrium can only be used to produce polyunsaturated fatty acids
[0005] The technical problem to be solved by the present invention is to provide a method for producing biodiesel feedstock oil, which overcomes the shortcomings of the existing fermentation process for producing biodiesel feedstock oil, such as long cycle, low yield, and complicated process.

Method used

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  • Method for producing raw oil of biodiesel
  • Method for producing raw oil of biodiesel
  • Method for producing raw oil of biodiesel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The production method of biodiesel raw material oil, comprises the following steps:

[0030] (1) prepare seed culture medium, sterilize for later use

[0031] Seed medium includes: glucose 40.0g / L, yeast powder 5.0g / L, peptone 1.0g / L, magnesium sulfate 0.5g / L, calcium bicarbonate 0.2g / L, potassium dihydrogen phosphate 1.0g / L, chloride Sodium 1.0g / L.

[0032] (2) Preparation of fermentation medium, sterilized for subsequent use

[0033] Fermentation medium includes: glucose 80.0g / L, corn steep liquor 10.0g / L, sodium glutamate 8.0g / L, magnesium sulfate 0.5g / L, calcium bicarbonate 1.0g / L, potassium dihydrogen phosphate 1.0g / L , sodium nitrate 5.0g / L.

[0034] (3) Preparation of seed solution

[0035] Inoculate Schizochytrium into the seed culture medium, the inoculum volume percentage is 2%, shake flask culture is carried out under the conditions of shaking table rotation speed 300rpm, culture temperature 28 ℃ for 24h, to obtain seed liquid.

[0036] (4) Fermentation ...

Embodiment 2

[0043] The production method of biodiesel raw material oil, comprises the following steps:

[0044] (1) prepare seed culture medium, sterilize for later use

[0045] Seed medium includes: glucose 60.0g / L, yeast powder 10.0g / L, peptone 5.0g / L, magnesium sulfate 2.0g / L, calcium bicarbonate 1.0g / L, potassium dihydrogen phosphate 3.0g / L, chloride Sodium 5.0g / L.

[0046] (2) Preparation of fermentation medium, sterilized for subsequent use

[0047] Fermentation medium includes: glucose 150.0g / L, corn steep liquor 30.0g / L, sodium glutamate 20.0g / L, magnesium sulfate 3.0g / L, calcium bicarbonate 5.0g / L, potassium dihydrogen phosphate 3.0g / L , sodium nitrate 10.0g / L.

[0048] (3) Preparation of seed solution

[0049] Inoculate Schizochytrium into the seed culture medium with an inoculum volume percentage of 5%, shake flask culture for 20 h under the conditions of a shaker rotation speed of 180 rpm and a culture temperature of 40° C. to obtain a seed solution.

[0050] (4) Fermenta...

Embodiment 3

[0057] The production method of biodiesel raw material oil, comprises the following steps:

[0058] (1) prepare seed culture medium, sterilize for later use

[0059] Seed medium includes: glucose 45.0g / L, yeast powder 6.0g / L, peptone 2.0g / L, magnesium sulfate 1.0g / L, calcium bicarbonate 0.4g / L, potassium dihydrogen phosphate 1.5g / L, chloride Sodium 2.0g / L.

[0060] (2) Preparation of fermentation medium, sterilized for subsequent use

[0061] Fermentation medium includes: glucose 100.0g / L, corn steep liquor 15.0g / L, sodium glutamate 10.0g / L, magnesium sulfate 1.0g / L, calcium bicarbonate 2.0g / L, potassium dihydrogen phosphate 1.5g / L , sodium nitrate 6.0g / L.

[0062] (3) Preparation of seed solution

[0063] Inoculate Schizochytrium into the seed culture medium with an inoculum volume percentage of 3%, shake flask culture for 22 hours under the conditions of a shaker rotation speed of 200 rpm and a culture temperature of 32° C. to obtain a seed solution.

[0064] (4) Fermen...

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Abstract

The invention relates to a method for producing raw oil of biodiesel. The method includes fermenting and cultivating schizochytrium to produce grease. The content of saturated fatty acid in the grease is higher than 50% and can be even higher than 70%. The method has the advantages that illumination and carbon dioxide filling can be omitted in fermentation procedures, fermentation temperatures, ventilation conditions and fed-batch feeding are controlled, accordingly, the biomass live-weights can reach 120g / L-150g / L in fermentation periods of 3-5 days, the grease content is higher than 75%, the method is high in efficiency and low in production cost, industrial production can be facilitated, and the like.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and renewable energy, and more specifically, relates to a production method of biodiesel raw material oil. Background technique [0002] Biodiesel, also known as biodiesel, is a renewable diesel fuel that can replace petrochemical diesel produced by transesterification using unprocessed or used vegetable oil, animal fat, and food waste oil as raw materials. With the gradual depletion of fossil fuels and the worsening of environmental problems, as a clean and environmentally friendly renewable energy, biodiesel has received more and more attention. [0003] At present, the main raw materials used to make biodiesel include vegetable oil scraps, urban waste oil, swill oil, microalgae oil, etc. Vegetable oil scraps, urban waste oil, swill oil and other waste oils are used as raw material oil for biodiesel production The method of biodiesel greatly reduces the harm to the environment and human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCY02E50/10
Inventor 余龙江陈伟朱圆敏金文闻余金龙陈祥明
Owner WUHAN HUASHITE IND BIOTECH DEV
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