Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Genetic markers of susceptibility to electromagnetic radiation

A technology of electromagnetic radiation and susceptibility, applied in the fields of genetic markers and drug screening, to achieve the effect of convenience and high accuracy of the kit

Active Publication Date: 2018-02-09
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, research on the discovery and function of SNPs still needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetic markers of susceptibility to electromagnetic radiation
  • Genetic markers of susceptibility to electromagnetic radiation
  • Genetic markers of susceptibility to electromagnetic radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 prepares in vitro experiment material

[0055] cell

[0056] Selection: 293T cell line (human kidney epithelial cell line), PC12 cell line (rat adrenal pheochromocytoma cell line). 293T cells are widely used for protein overexpression and transient transfection because of their high protein expression level and high cell transfection efficiency; PC12 cells can be used as a mature neuron model after being induced by nerve growth factor, and are widely used in neurophysiological pathology Research, the choice of PC12 cells is conducive to further research in the later stage.

[0057] 293T cell culture medium is DMEM medium, containing 10% fetal bovine serum and 100U / ml penicillin and streptomycin. Cells were passaged by trypsinization. PC12 cells are semi-adherent cells, and their culture flasks and culture plates are coated with poly-lysine solution (concentration: 0.1 mg / ml) before use. The cell culture medium is: DMEM culture medium, containing 5% feta...

Embodiment 2

[0066] Embodiment 2 reporter gene experiment

[0067] The reporter gene experiment was used to detect the difference in promoter activity of different alleles of rs198585630 and the effect of microwave radiation on the promoter activity of different alleles.

[0068] 2.1 Construction of pGL3-basic-rs198585630-T and pGL3-basic-rs198585630-C recombinant plasmids

[0069] Rat HT 1A R gene upstream -967bp to the start codon ATG as its promoter region, synthesized 967bp of promoter target fragments including rs198585630-T and rs198585630-C respectively, and introduced restriction endonuclease sites upstream and downstream: 5' -GGTACC-3' and 5'-CTCGAG-3' (the endonucleases are KpnI and XhoI respectively), a total of 979bp, HT introduced into the endonuclease site 1A The promoter sequence of R is as figure 1 As shown, the T / C allele at position 753 at the 5' end of the promoter sequence, the KpnI restriction site upstream of the promoter, and the XhoI restriction site downstream o...

Embodiment 3

[0101] Example 3 Electrophoretic mobility shift assay (EMSA)

[0102] For the promoter fragment containing rs198585630 site, Gene-regulation and MatInspector online database and program were used to predict. The most likely transcriptional regulator binding to this SNP site is Nkx-2.5 / Csx, and the transcriptional factor binding site (Transcription factor binding site, TFBS) is gcctcTGAGtgct[t / c]aggac (SNP rs198585630 in square brackets) . Biotin-labeled and non-biotinylated nucleotide duplexes containing rs198585630 T allele and C allele were synthesized, respectively. The TFBS sequence is shown in Table 3 (the circled bases are SNP sites):

[0103] table 3

[0104]

[0105]

[0106] The PC12 cells were divided into radiation group and sham radiation group, and the coated 25cm culture flasks were used for cell culture, and the cells were placed on the radiation table for microwave radiation or sham radiation, and the cells were collected after 1 hour.

[0107] The nu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides genetic markers of susceptibility to electromagnetic radiation. Specifically, the present invention provides a mutant, an expression cassette, a detection reagent, a kit, a disease model, and a method for screening drugs method. Compared with the nucleotide sequence shown in SEQ ID NO: 1, the 753rd base of the mutant is mutated to C. This mutation site is related to susceptibility to microwave radiation.

Description

technical field [0001] The present invention relates to the field of biotechnology, specifically, the present invention relates to genetic markers of susceptibility to electromagnetic radiation, more specifically, the present invention relates to a mutant, an expression cassette, a detection reagent, a kit, a A disease model and a method for screening drugs. Background technique [0002] SNP (Single Nucleotide Polymorphism) is a kind of mutant, which refers to the variation of a single nucleotide on the genome. SNP loci are widely distributed in the human genome, but only a small part of them have functional significance. SNP sites can be distributed in the coding region or non-coding region of the genome, and the SNP sites at different positions have different functions: SNPs at the junction of exons and introns may change their splicing forms, resulting in differences in their biological functions; Some SNPs in the coding region can change the amino acid sequence and aff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N5/10C12Q1/68G01N33/68
Inventor 王丽峰胡向军李海娟王长振邹勇彭瑞云高亚兵智维佳
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com