Silk-screen printing electrode sensor preparation method based on ionic liquid-multi-wall carbon nano-tube modification
A technology of multi-walled carbon nanotubes and screen-printed electrodes, applied in the field of biosensors, can solve the problems of low residue and quantitative detection, the influence of spectrophotometry, and inaccurate detection results, and achieve excellent functionality and compatibility High performance, high stability and sensitivity, high chemical stability and thermal stability
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Embodiment 1
[0052] Example 1: A method for preparing a sensor based on screen-printed carbon electrodes modified by ionic liquid-multi-walled carbon nanotubes
[0053] (1) Cleaning of the screen-printed carbon electrode before the preparation of the amperometric acetylcholinesterase sensor. Place the screen-printed carbon electrode in 1mM NaOH solution for 5min, rinse with ultrapure water, N 2 blow dry. Afterwards, the electrode was placed in 1mM HCl solution for 5min sonication, rinsed with ultrapure water, N 2 blow dry. Finally, rinse the electrode with ethanol, N 2 Blow dry; (2) Activation of the electrode before the preparation of the amperometric acetylcholinesterase sensor. Scan the current-time curve for 300s in pH5.0 phosphate buffer, and then scan the cyclic voltammetry curve until the performance is stable; (3) Preparation of ionic liquid / multi-walled carbon nanotubes. Disperse 4mg of N-octylpyridinium hexafluorophosphate (ionic liquid) in 4mL of absolute ethanol and disper...
Embodiment 2
[0054] Example 2: Using the prepared amperometric acetylcholinesterase sensor to detect vegetable samples
[0055] (1) The acetylcholinesterase sensor prepared according to Example 1 was subjected to a cyclic voltammetry test at a scan rate of 50 mV / s in a phosphate buffer solution of pH 7.5 containing 1.0 mM thioacetylcholine chloride (ATCl), The potential window is 0V~1.0V; (2) Prepare chlorpyrifos standard solution. When measuring pesticides, immerse the above-mentioned acetylcholinesterase sensor in different concentrations of pesticide standard solutions for 10 minutes, and then add 15~20mL containing 1.0mM chlorine to the reaction pool. Thioacetylcholine (ATCl) in phosphate buffered saline solution, carry out cyclic voltammetry scanning, and the inhibition rate I can be obtained by the following formula:
[0056] I(%)=(I P, control ?I P, exp ) / I P, control ×100%
[0057] where I P, control and I P, exp Respectively, the peak current of the modified electrode in the...
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