Preparation method and application of immunosensor constructed by difunctional marker
An immunosensor and marker technology, which can be used in analytical materials, biological tests, instruments, etc., to achieve the effect of increasing the current response
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Embodiment 1
[0042] 1. A method for preparing a bifunctional marker-secondary antibody hatch, comprising the following steps:
[0043] (1) Preparation of titanium dioxide nanomaterials
[0044] Add 2 mL of butyl titanate to 40 mL of ethylene glycol, stir at room temperature for 6 h to form a mixture, add this mixture to 150 mL of acetone, add 2 mL of water, stir vigorously for 1 h, centrifuge, wash with ethanol for 2 Once, dry at 50°C to obtain the titanium dioxide precursor; disperse 0.1 g of the titanium dioxide precursor into 20 mL of water, heat and reflux for 1 h under stirring; then centrifuge, wash with ethanol twice, and dry at 50°C to obtain the titanium dioxide nanometer material, spare;
[0045] (2) Copper doped titanium dioxide CuTiO 2 preparation of
[0046] Add 1 g of titania nanomaterials to 40 mL, 10 mol L -1 in NaOH solution, stirred for 2 h, put into a polytetrafluoroethylene reaction kettle, heated at 120°C for 18 h, took it out, cooled to room temperature, and used ...
Embodiment 2
[0052] 1. A method for preparing a bifunctional marker-secondary antibody hatch, is characterized in that, comprises the following steps:
[0053] (1) Preparation of titanium dioxide nanomaterials
[0054] Add 2 mL of butyl titanate to 50 mL of ethylene glycol, stir at room temperature for 8 h to form a mixture, add the mixture to 170 mL of acetone, add 3 mL of water, stir vigorously for 2 h, centrifuge, and wash with ethanol 4 times , dried at 50°C to obtain a titanium dioxide precursor; disperse 0.1 g of the titanium dioxide precursor into 20 mL of water, heat and reflux for 2 h under stirring conditions; then centrifuge, wash with ethanol four times, and dry at 50°C to obtain titanium dioxide nanomaterials ,spare;
[0055] (2) Copper doped titanium dioxide CuTiO 2 preparation of
[0056] Add 1 g of titania nanomaterials to 40 mL, 10 mol L -1 in NaOH solution, stirred for 3 h, put into a polytetrafluoroethylene reaction kettle, heated at 140°C for 24 h, took it out, coo...
Embodiment 3
[0062] 1. A method for preparing a bifunctional marker-secondary antibody hatch, comprising the following steps:
[0063] (1) Preparation of titanium dioxide nanomaterials
[0064] Add 2 mL of butyl titanate to 45 mL of ethylene glycol, stir at room temperature for 7 h to form a mixture, add the mixture to 160 mL of acetone, add 2.5 mL of water, stir vigorously for 1.5 h, centrifuge, wash with ethanol three times, Dry at 50°C to prepare the titanium dioxide precursor; disperse 0.1 g of the titanium dioxide precursor into 20 mL of water, heat and reflux for 1.5 h under stirring conditions; then centrifuge, wash with ethanol three times, and dry at 50°C to prepare titanium dioxide nanomaterials. spare;
[0065] (2) Copper doped titanium dioxide CuTiO 2 preparation of
[0066] Add 1 g of titania nanomaterials to 40 mL, 10 mol L -1 in NaOH solution, stirred for 2.5 h, put it into a polytetrafluoroethylene reaction kettle, heated at 130°C for 20 h, took it out, cooled to room t...
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