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Production method of NAD fermentation liquor

A production method and technology of fermented liquid, applied in the production field of NAD fermented liquid, can solve the problems of high price, high price and harsh conditions of NAD, and achieve the effect of promoting mass accumulation, low cost and simple operation

Inactive Publication Date: 2015-11-04
HEFEI PINGGUANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, NAD production is mainly through two ways: (1) biocatalytic synthesis, modern biocatalytic technology simulates the reaction completed by using enzymes in the organism, and the simulation conditions are strictly based on the metabolic characteristics of the organism, so Harsh conditions, low yield and high price; (2) Yeast is directly fermented and synthesized, and the medium prepared with glucose, yeast extract, peptone, ammonium sulfate, etc. is fermented to obtain yeast, and then separated by wall breaking and other processes. The output of NAD is about 1g / l, and the low content of NAD is also an important reason for the high price of NAD in the current market

Method used

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Examples

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Effect test

Embodiment 1

[0025] A production method of NAD fermentation liquid, comprising the steps of:

[0026] S1. Preparation of soft tissue protein extracts of apple snails: mix soft tissue powder of apple snails and Bacillus subtilis neutral protease phosphate buffer solution with a concentration of 4wt% at a weight ratio of 1:30, heat up to 50°C, keep warm for 7 hours, and filter. Get supernatant, spray dry to obtain apple snail soft tissue protein eluate, wherein, the pH=7.0 of phosphate buffer, containing the NaCl of 140mmol / l, the KCl of 2.4mmol / l, the NaH of 2mmol / l 2 PO 4 and 7mmol / l Na 2 HPO 4 ;

[0027] S2. Preparation of cassava saccharification liquid: mix cassava flour and water, heat up to 80°C, keep warm for 30 minutes, stir continuously during the heat preservation process, add α-amylase, keep heat for 25 minutes, heat up to 93°C, keep heat for 80 minutes, and cool down to 55 ℃, add saccharification solution with glucoamylase activity of 6000IU / g, keep warm for 45min, adjust so...

Embodiment 2

[0030] A production method of NAD fermentation liquid, comprising the steps of:

[0031]S1. Preparation of soft tissue protein extracts of apple snails: mix soft tissue powder of apple snails and Bacillus subtilis neutral protease phosphate buffer solution with a concentration of 6wt% at a weight ratio of 1:20, heat up to 54°C, keep warm for 6 hours, and filter. Take supernatant, spray dry to obtain apple snail soft tissue protein eluate, wherein, the pH=7.3 of phosphate buffer, contain the NaCl of 130mmol / l, the KCl of 3mmol / l, the NaH of 1mmol / l 2 PO 4 and 9mmol / l Na 2 HPO 4 ;

[0032] S2. Preparation of cassava saccharification liquid: mix cassava flour with water, heat up to 75°C, keep warm for 40 minutes, keep stirring during the heat preservation process, add α-amylase, keep heat for 20 minutes, heat up to 95°C, keep heat for 70 minutes, and cool down to 56 ℃, add saccharification solution with glucoamylase activity of 4000IU / g, keep it warm for 50min, adjust the con...

Embodiment 3

[0035] A production method of NAD fermentation liquid, comprising the steps of:

[0036] S1. Preparation of soft tissue protein extracts of apple snails: mix soft tissue powder of apple snails and Bacillus subtilis neutral protease phosphate buffer solution with a concentration of 4.5wt% in a weight ratio of 1:28, heat up to 51°C, and keep warm for 6.8 hours. Filtrate, take the supernatant, and spray-dry to obtain the soft tissue protein extract of apple snails, wherein the pH of the phosphate buffer is 7.1, containing 138mmol / l of NaCl, 2.6mmol / l of KCl, and 1.7mmol / l of NaH 2 PO 4 and 7.5mmol / l of Na 2 HPO 4 ;

[0037] S2. Preparation of cassava saccharification liquid: mix cassava flour with water, heat up to 79°C, keep warm for 32 minutes, keep stirring during the heat preservation process, add α-amylase, keep heat for 24 minutes, heat up to 93.5°C, keep heat for 78 minutes, and cool down to 55 ℃, add saccharification solution with glucoamylase activity of 5500IU / g, ke...

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Abstract

The invention discloses a production method of NAD fermentation liquor. The method includes the steps of S1, well mixing crushed ampullaria crossean soft tissue and bacillus subtilis neutral proteinase, performing enzymolysis, filtering, and taking supernate and drying to obtain ampullaria crossean soft tissue protein dissolved matter; S2, well mixing cassava starch and water, adding alpha-amylase for liquefaction, and then adding saccharification liquid for saccharification to obtain cassava saccharification liquid; S3, well mixing the ampullaria crossean soft tissue protein dissolved matter and the cassava saccharification liquid to obtain solution A, adding vitamin complex liquid and well mixing to obtain solution B, sterilizing, cooling to room temperature, adding fermentation strains, heating to 28-32 DEG C, and keeping the temperature for 36-48 hours to obtain the NAD fermentation liquor. The method has the advantages that NAD accumulation amount in the fermentation liquor is increased greatly and can reach 4.5-6.5g / l, and the method is cheap in raw materials, easy in raw material obtaining, low in cost, simple to operate and suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a production method of NAD fermentation liquid. Background technique [0002] Nicotinamide adenine dinucleoside (NAD) is an essential multifunctional small molecule for organisms, mainly serving as an important coenzyme for oxidoreductases and a source of NADP. The multi-enzyme redox system in which NAD(H) participates is the main biological oxidation system in the electron transfer process in the respiratory chain of living organisms. Most of the oxidation reactions in the decomposition of the three major metabolites of sugar, lipid and protein are also through this system. The system is completed. NAD + , NADP, NADH and NADPH act as the acceptor and donor of hydride, which play a key role in the energy metabolism of cells and have great application value in the field of medicine. [0003] At present, NAD production is mainly through two ways: (1) biocatalytic synthesis,...

Claims

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Application Information

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IPC IPC(8): C12P19/40C12R1/865
Inventor 王康林杨培周金永红杨杨
Owner HEFEI PINGGUANG PHARMA
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