Application of optimized arabidopis thaliana glycosyl transferase gene in preparation of isoquercitrin

A technology of Arabidopsis thaliana sugar and isoquercetin, which is applied in the fields of genetic engineering technology and biomedicine, can solve the problems of high cost, difficult preparation, low yield and the like, and achieves high specificity, simple production method and high purity high effect

Inactive Publication Date: 2015-10-21
NANJING FORESTRY UNIV
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  • Description
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Problems solved by technology

Invention patent (publication number: CN 1355797A) discloses a method for recovering isoquercetin from bioflavonoids, but the natural average content of isoquercetin is very low, and it is difficult to prepare by means of extraction and separation, with low yield and low cost. high

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  • Application of optimized arabidopis thaliana glycosyl transferase gene in preparation of isoquercitrin
  • Application of optimized arabidopis thaliana glycosyl transferase gene in preparation of isoquercitrin
  • Application of optimized arabidopis thaliana glycosyl transferase gene in preparation of isoquercitrin

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Embodiment 1

[0019] 1. Construction of recombinant plasmid PGEX-2T-OPAP73B3

[0020] 1.1 Arabidopsis glycosyltransferase gene ap73B3 Optimization

[0021] Refer to Arabidopsis thaliana published by NCBI ( Arabidopsis thaliana ) cDNA sequence of glycosyltransferase 73B3 (AP 73B3), the nucleotide sequence information of glycosyltransferase AP 73B3 is obtained, as shown in SEQ NO:1, and the amino acid sequence is shown in SEQ NO:2. According to the Escherichia coli K12 dominant codon table (http: / / www.kazusa.or.jp / codon / ), the gene sequence of glycosyltransferase AP 73B3 was optimized, and codons with high usage frequency of amino acid synonymous codons were selected as dominant codons, and finally the optimized Arabidopsis glycosyltransferase gene ( opap73B3 ) sequence information, as shown in SEQ NO:3. After obtaining the sequence information, it was synthesized by Shanghai Jierui Bioengineering Co., Ltd. to obtain the optimized Arabidopsis thaliana glycosyltransferase gene opap73B3...

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Abstract

The invention discloses an application of an optimized arabidopis thaliana glycosyl transferase gene in the preparation of isoquercitrin, and the nucleotide sequence of the gene is represented by the SEQ No.3. The provided glycosyl transferase has high specificity, and is capable of specifically converting quercetin into isoquercitrin. The provided isoquercitrin production method is simple and the purity of obtained isoquercitrin is high.

Description

technical field [0001] The invention belongs to the field of genetic engineering technology and biomedicine, and specifically relates to an optimized Arabidopsis glycosyltransferase gene and its application in preparing isoquercetin. Background technique [0002] Flavonoids are widely found in Ginkgo biloba, Sophora japonica, Hippophae rhamnoides, Eucalyptus, Scutellaria baicalensis and other plants, and have a wide range of pharmacological activities, including anti-oxidation, anti-inflammatory, anti-allergic, antibacterial, immune regulation and cardiovascular protection. Studies have shown that compared with rutin and quercetin, isoquercetin (quercetin-3-O-glucoside) has stronger biological activity in many aspects. For example, isoquercetin is superior to rutin and quercetin in inhibiting the proliferation of malignant tumors, HIV-1 and HSV-1 / 2, and is superior to quercetin in inhibiting the activity of acetylcholinesterase. Therefore, isoquercetin has great applicati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/70C12N1/21C12P19/60
Inventor 裴建军赵林果董萍吴涛
Owner NANJING FORESTRY UNIV
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