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Determination method for content of mussaendoside G in medicinal material Mussaenda pubescens

A kind of technology of golden anthocyanidin and golden flower, applied in the field of content determination of golden anthoside G and golden flower of golden leaf in medicinal materials, can solve the problems such as no content determination, achieve good reproducibility, accurate measurement result, high precision effect

Inactive Publication Date: 2015-10-07
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yuye Jinhua is recorded in the first volume of "Quality Standards of Zhuang Medicine of Guangxi Zhuang Autonomous Region". There are only physical and chemical identifications in the standards, and only TLC identifications in other patented medicines. There is no relevant report on its content determination.

Method used

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  • Determination method for content of mussaendoside G in medicinal material Mussaenda pubescens
  • Determination method for content of mussaendoside G in medicinal material Mussaenda pubescens
  • Determination method for content of mussaendoside G in medicinal material Mussaenda pubescens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Determination of the content of aureoside G in Jade japonica medicinal material (National Inspection Institute reference medicinal material 121404-200401)

[0062] According to the "Chinese Pharmacopoeia" 2010 edition an appendix VID high performance liquid chromatography determination operation, the steps are as follows:

[0063] Chromatographic conditions and system suitability experiment

[0064] Use C18 bonded silica gel as the filler; use acetonitrile:water=38:62 as the mobile phase; the flow rate is 1ml min -1 ;The column temperature is 25°C; the detection wavelength is 265nm; the number of theoretical plates should not be less than 3000;

[0065] Preparation of reference solution

[0066] Accurately weigh an appropriate amount of the reference substance of euphyllin G, and make 0.60mg·ml with methanol -1 solution;

[0067] Preparation of the test solution

[0068] Take 3.0g of Yuye Jinhua medicinal material powder (passing 40 mesh), weigh it acc...

Embodiment 2

[0072] Determination of the content of Jade leaf aureoside G in embodiment 2 Jade leaf golden flower medicinal material (Bobai County, Guangxi)

[0073] According to the "Chinese Pharmacopoeia" 2010 edition an appendix VID high performance liquid chromatography determination operation, the steps are as follows:

[0074] Chromatographic conditions and system suitability experiment

[0075] Use C18 bonded silica gel as the filler; use acetonitrile:water=35:65 as the mobile phase; the flow rate is 1ml min -1 ;The column temperature is 30°C; the detection wavelength is 268nm; the number of theoretical plates should not be less than 3000;

[0076] Preparation of reference solution

[0077] Accurately weigh an appropriate amount of the reference substance of euphyllin G, and make 0.06mg·ml with methanol -1 solution;

[0078] Preparation of the test solution

[0079] Take 2.0g of Yuye Jinhua medicinal material powder (passing 40 mesh), weigh it accurately, put it in a stopper...

Embodiment 3

[0083] Determination of the content of euphyllin G in embodiment 3 euphyllum japonica medicinal material (Jinxiu County, Guangxi)

[0084] According to the "Chinese Pharmacopoeia" 2010 edition an appendix VID high performance liquid chromatography determination operation, the steps are as follows:

[0085] Chromatographic conditions and system suitability experiment

[0086] Use C18 bonded silica gel as the filler; use acetonitrile:water=25:75 as the mobile phase; the flow rate is 1ml min -1 ;The column temperature is 30°C; the detection wavelength is 270nm; the number of theoretical plates should not be less than 3000;

[0087] Preparation of reference solution

[0088] Accurately weigh an appropriate amount of the reference substance of euphyllin G, and make 0.30mg·ml with methanol -1 solution;

[0089] Preparation of the test solution

[0090] Take 5.0g of Yuye Jinhua medicinal material powder (passing 40 mesh), weigh it accurately, put it in a stoppered Erlenmeyer ...

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Abstract

The invention discloses a determination method for the content of mussaendoside G in the medicinal material Mussaenda pubescens. According to the invention, high-performance liquid chromatographic determination is carried out with the Mussaenda pubescens glycosides G as a contrast and a mixture of acetonitrile and water / 0.01% phosphoric acid as a mobile phase, wherein the ratio of acetonitrile to water / 0.01% phosphoric acid is 25-40: 60-75. The method perfects quality testing standards of the medicinal material Mussaenda pubescens and lays a foundation for establishment of the quality standards of the medicinal material Mussaenda pubescens. The determination method provided by the invention selects the mussaendoside G in the medicinal material Mussaenda pubescens as an index for content determination, has the advantages of high precision, good reproducibility, good stability, high recovery rate and accurate determination results, and can effectively control the quality of the medicinal material Mussaenda pubescens so as to ensure safety and effectiveness of clinical medication of the medicinal material Mussaenda pubescens.

Description

technical field [0001] The invention belongs to the technical field of quality control of medicinal materials, and relates to aureoside G, in particular to a method for determining the content of aureoside G in the medicinal material Aurea japonica. Background technique [0002] Mussaenda pubescens Ait.f. is the dry stem and root of Mussaenda pubescens Ait.f., which has the effects of clearing away heat and relieving heat, cooling blood and detoxifying. It is widely used in Lingnan area, mainly for treating colds, heatstroke, nephritis, edema, For sore throat, bronchitis and other diseases, Yuye Jiedu Granules, a well-known domestic brand of clearing fire and anti-inflammatory drugs, and Yuye Jinhua in Yuye Qinghuo Tablets are all used as monarch drugs. [0003] It is reported in the literature that the medicinal material of Jade Yejinhua mainly contains terpenoids, triterpenoid saponins and other components. Yuyejinhua is recorded in the first volume of "Quality Standards ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 张赟赟李嘉张颖陈锋杨海船何春欢李红姜平川
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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