Method for detecting concentration of ferrohemoglobin by using fluorescent carbon dot probe

A technology of hemoglobin concentration and fluorescent carbon dots, which is applied in the field of detection of hemoglobin concentration using fluorescent carbon dot probes, to achieve the effects of good biocompatibility, easy-to-obtain raw materials, and low price

Inactive Publication Date: 2015-10-07
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there are no relevant reports on the use of fluorescent carbon dots as probes for hemoglobin detection.

Method used

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  • Method for detecting concentration of ferrohemoglobin by using fluorescent carbon dot probe
  • Method for detecting concentration of ferrohemoglobin by using fluorescent carbon dot probe

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Experimental program
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Effect test

Embodiment 1

[0034] 1. Synthesis of fluorescent carbon dots

[0035] Fluorescent carbon dots were synthesized by microwave heating, using polyethylene glycol with a molecular weight of 1500, glycerol and serine as raw materials: 1.0 g of polyethylene glycol with a molecular weight of 1500 and 15 mL of glycerin were stirred and placed in a microwave reactor at 140 ° C for high temperature reaction After 15 minutes, when the test tube was cooled to 50°C, 1.0 g of serine was added, and the temperature was raised to 180°C for 10 minutes of microwave reaction. The prepared sample was injected into a dialysis bag with a molecular weight of 1000 for dialysis and then evaporated and concentrated for 24 hours to obtain 300 mL of dialysis The dialysate was rotary evaporated at a constant temperature of 60°C for one hour, and finally 260 mL of fluorescent carbon dots were obtained. The quantum yield of the above fluorescent carbon dots is about 13% by using quinine sulfate as a standard substance.

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Embodiment 2

[0043] A method for detecting hemoglobin concentration using a fluorescent carbon dot probe, comprising:

[0044]Step 1, preparing standard solutions of hemoglobin containing fluorescent carbon dots in different concentrations, detecting the fluorescence intensity of the standard solution, obtaining the fluorescence spectrum of the standard solution, and establishing the fluorescence intensity of the standard solution with a hemoglobin concentration of zero and all standard solutions The linear relationship between the difference of the fluorescence intensity of the solution and the concentration of hemoglobin;

[0045] Step 2: Prepare a sample solution of hemoglobin containing fluorescent carbon dots, detect the fluorescence intensity of the sample solution, and determine the concentration of hemoglobin in the sample solution through the linear relationship.

[0046] In the method for detecting hemoglobin concentration using fluorescent carbon dot probes, the preparation meth...

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Abstract

The invention discloses a method for detecting the concentration of ferrohemoglobin by using a fluorescent carbon dot probe. The method comprises the following steps: 1, preparing different concentrations of fluorescent carbon dot-containing ferrohemoglobin standard solutions, detecting the fluorescence intensity of the standard solutions to obtain a standard solution fluorescence spectrogram, and establishing a linear relation between a difference between the fluorescence intensity of the standard solution with the ferrohemoglobin concentration being 0 and the fluorescence intensity of every standard solution and the ferrohemoglobin concentrations; and 2, preparing a fluorescent carbon dot-containing ferrohemoglobin sample solution, detecting the fluorescence intensity of the sample solution, and determining the concentration of ferrohemoglobin in the sample solution through the linear relation. The ferrohemoglobin concentration is detected by using the characteristics of a ferrohemoglobin quenching fluorescence carbon dot with the fluorescence carbon dot as a probe, so the method has the advantages of simple and convenient detection process, high sensitivity and low detection limit, and can realize online, in-situ, rapid and sensitive detection of the concentration of ferrohemoglobin in a practical sample.

Description

technical field [0001] The invention relates to a method for detecting the concentration of hemoglobin, in particular to a method for detecting the concentration of hemoglobin by using a fluorescent carbon point probe. Background technique [0002] Hemoglobin exists in large quantities in red blood cells and is the main component of oxygen transport in the blood. It plays a role in transporting oxygen and decomposing H in the body. 2 o 2 Important activities related to oxygen and energy metabolism, such as transfer of electrons, play a key role in all life activities. The methods for the determination of hemoglobin include visible photometry, chemiluminescence and electrochemical methods. However, these methods have disadvantages such as cumbersome pretreatment process and long analysis time. [0003] In recent years, fluorescent carbon dots are one of the most popular carbon nanomaterials after fullerenes, carbon nanotubes and graphene. This nanomaterial overcomes some ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 肖琦黄珊王鲁敏谢江宁黄初升盛家荣
Owner GUANGXI TEACHERS EDUCATION UNIV
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