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Indocyanine green composite nanoparticle as well as preparation method and application thereof

A composite nanoparticle, indocyanine green technology, applied in the field of nanomaterials, can solve the problems of lack of tumor cell targeting specificity, limited application of indocyanine green, short circulation time, etc., to avoid decomposition and in vivo clearance, increase The effect of targeting recognition and improving stability

Active Publication Date: 2015-09-23
珠海中科先进技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, indocyanine green will quickly aggregate in polar solvents, and its stability is poor. It will be quickly cleared in plasma, and the circulation time in the body is short. At the same time, indocyanine green also lacks tumor cell targeting specificity. These Defects greatly limit the application of indocyanine green

Method used

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  • Indocyanine green composite nanoparticle as well as preparation method and application thereof
  • Indocyanine green composite nanoparticle as well as preparation method and application thereof
  • Indocyanine green composite nanoparticle as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0079] A preparation method of indocyanine green composite nanoparticles, comprising the following steps:

[0080] (1) Extract cancer cell membrane: take cancer cells, add hypotonic buffer solution, homogeneously break up, and collect the precipitate by centrifugation several times, that is, cancer cell membrane;

[0081] Human breast cancer cells (MCF-7) were inoculated in T-175 cell culture flasks, cultured with DMEM medium, and added with 10% FBS, 1% penicillin, and 1% streptomycin. After the cells were confluent, The old medium was discarded, digested with EDTA, centrifuged and washed 3 times with PBS to obtain the target cancer cells;

[0082] Add 20mM Tris-HCl hypotonic buffer solution with pH=7.5 to the washed MCF-7 cells, the hypotonic buffer solution also contains 10mM KCl, 2mM MgCl 2 , 1.0 μg / ml protease inhibitor, use a Dounce homogenizer to homogenize the cells for 20 times, centrifuge at a low speed of 3200g for 5 minutes, save the supernatant, and add hypotonic ...

Embodiment 2

[0090] A preparation method of indocyanine green composite nanoparticles, comprising the following steps:

[0091] (1) Extract cancer cell membrane: the steps are the same as in Example 1;

[0092] (2) Preparation of vesicle structure: the ratio of the extracted cancer cell membrane to DSPE-PEG (where the molecular weight of PEG is 400) is 3×10 8 : 180 μg was weighed, and dissolved in 1ml, 4% ethanol, and ultrasonic cell disruptor was used to ultrasonicate for 5min at a frequency of 20kHz and a power of 35W under an ice bath to obtain the first mixed solution;

[0093] (3) Preparation of a spherical inner core: dissolving indocyanine green (ICG) in ethanol to obtain a 0.5 mg / ml ICG solution, dissolving polylactic acid-glycolic acid copolymer (PLGA) in acetonitrile to obtain a 0.5 mg / ml PLGA solution , take 1.5ml of ICG solution, and ultrasonicate it, while adding 1ml of PLGA solution dropwise into 1.5ml of ICG solution, ultrasonic time is 2min, to obtain the second mixed solu...

Embodiment 3

[0096] A preparation method of indocyanine green composite nanoparticles, comprising the following steps:

[0097] (1) Extract cancer cell membrane: the steps are the same as in Example 1;

[0098] (2) Preparation of vesicle structure: the ratio of the extracted cancer cell membrane to DSPE-PEG (wherein the molecular weight of PEG is 20000) is 9×10 8 : 180 μg was weighed, and dissolved in 1ml, 4% ethanol, and ultrasonic cell disruptor was used to ultrasonicate for 5min at a frequency of 20kHz and a power of 35W under an ice bath to obtain the first mixed solution;

[0099] (3) Preparation of a spherical inner core: dissolving indocyanine green (ICG) in ethanol to obtain a 0.5 mg / ml ICG solution, dissolving polylactic-co-glycolic acid (PLGA) in acetonitrile to obtain a 2 mg / ml PLGA solution, Take 1.5ml of ICG solution and ultrasonicate it, while adding 1ml of PLGA solution dropwise into 1.5ml of ICG solution, ultrasonic time is 2min, to obtain the second mixed solution;

[01...

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Abstract

The invention provides an indocyanine green composite nanoparticle which comprises indocyanine green, a hydrophobic polymer, polyethylene glycol derived phospholipid and a cancer cell membrane. The indocyanine green is encircled by the hydrophobic polymer and forms a sphere-like structure together with the hydrophobic polymer; the polyethylene glycol derived phospholipid penetrates the cancer cell membrane and forms a vesicle structure together with the cancer cell membrane; the sphere-like structure is wrapped in the vesicle structure. The invention further provides a preparation method and application of the indocyanine green composite nanoparticle. When the indocyanine green composite nanoparticle is applied, the indocyanine green composite nanoparticle has an efficient tumor targeting property, the indocyanine green can hardly gather and high stability is realized.

Description

technical field [0001] The invention belongs to the field of nanometer materials, in particular to an indocyanine green composite nanoparticle and its preparation method and application. Background technique [0002] Indocyanine Green (ICG) is a hydrogen tricarbonate dye, its maximum absorption wavelength and maximum emission wavelength are in the near-infrared region of 740nm and 800nm, and its fluorescence penetration is strong. It is a reagent approved by FDA (FDA) for human medical imaging and diagnosis, and it is used as a photothermal preparation for clinical hyperthermia. However, indocyanine green will quickly aggregate in polar solvents, and its stability is poor. It will be quickly cleared in plasma, and the circulation time in the body is short. At the same time, indocyanine green also lacks tumor cell targeting specificity. These The defect greatly limits the application of indocyanine green. Contents of the invention [0003] In order to solve the above prob...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/46A61K47/34A61K9/14A61P35/00
Inventor 蔡林涛陈泽郑明彬赵鹏飞罗震宇龚萍
Owner 珠海中科先进技术研究院有限公司
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