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Forming method of ampicillin and sulfadimethoxine electrochemical sensor self-assembled passivation layer, and electrochemical sensor thereof

A technology of sulfadisoxine and ampicillin, applied in the field of biological analysis, achieves the effects of sensitive detection, simple design and strong versatility

Active Publication Date: 2015-09-16
CAPITAL NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve the problem that the detection of ampicillin and sulfidexoxine cannot be realized when the MCH self-assembled passivation layer commonly used in the prior art is used in SD-EAB

Method used

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  • Forming method of ampicillin and sulfadimethoxine electrochemical sensor self-assembled passivation layer, and electrochemical sensor thereof
  • Forming method of ampicillin and sulfadimethoxine electrochemical sensor self-assembled passivation layer, and electrochemical sensor thereof
  • Forming method of ampicillin and sulfadimethoxine electrochemical sensor self-assembled passivation layer, and electrochemical sensor thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056]Embodiment 1: be used for the preparation of the SD-EAB A that ampicillin detects, self-assembly passivation layer is respectively OEG 6 -OMe.

[0057] Rinse the gold disc electrode (2 mm in diameter) with ultrapure water, and wash it with 1 μm, 0.3 μm, 0.05 μm Al 2 o 3 Polish the surface of the electrode with polishing powder (add a small amount of ultrapure water and solid powder on the polishing cloth and grind for 5-10 minutes), rinse with ultrapure water after each polishing, and ultrasonically in ultrapure water for 5 minutes before proceeding to the next grinding step. Ground smooth electrodes on a multi-channel potentiometer at 0.5M H 2 SO 4 The range of -0.4 ~ 1.2V is 100mV / s for 36 cycles of cyclic voltammetry scanning, the saturated mercurous sulfate electrode is used as the reference electrode, and the platinum electrode is used as the counter electrode until the cyclic voltammogram is basically stable. If no obvious corresponding redox peak is observed,...

Embodiment 2

[0059] Embodiment 2: the preparation of the sensor that is used for ampicillin detection, self-assembly passivation layer is respectively HS-(CH 2 ) 11 -(OCH 2 CH 2 ) 6 -COOH or MCH.

[0060] The self-assembled molecule in embodiment 1 is by OEG 6 -OMe was replaced by 1mM MCH or 1mMHS-(CH 2 ) 11 -(OCH 2 CH 2 ) 6 -COOH. Other steps are the same.

Embodiment 3

[0061] Embodiment 3: be used for the preparation of the SD-EAB B that ampicillin detects, self-assembly passivation layer is respectively OEG 6 -OMe.

[0062] Rinse the gold disc electrode (2 mm in diameter) with ultrapure water, and wash it with 1 μm, 0.3 μm, 0.05 μm Al 2 o 3 Polish the surface of the electrode with polishing powder (add a small amount of ultrapure water and solid powder on the polishing cloth and grind for 5-10 minutes), rinse with ultrapure water after each polishing, and ultrasonically in ultrapure water for 5 minutes before proceeding to the next grinding step. Ground smooth electrodes on a multi-channel potentiometer at 0.5M H 2 SO 4 The range of -0.4 ~ 1.2V is 100mV / s for 36 cycles of cyclic voltammetry scanning, the saturated mercurous sulfate electrode is used as the reference electrode, and the platinum electrode is used as the counter electrode until the cyclic voltammogram is basically stable. If no obvious corresponding redox peak is observed...

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Abstract

The invention relates to a forming method of a self-assembled passivation layer of a signal probe chain substitution-based aptamer electrochemical sensor (SD-EAB) for detecting ampicillin and sulfadimethoxine, and an electrochemical sensor thereof. A preparation method of ampicillin SD-EAB comprises the following steps: hybridizing a capture probe with the terminal modified by a mercapto group with a ferrocene labeled aptamer signal probe complementing with the capture probe, fixing the obtained hybridized probe on a gold electrode through self-assembling and closing the electrode by using OEG6-OMe as a self-assembled passivation layer molecule. The steps of a preparation method of sulfadimethoxine SD-EAB are similar to above steps. The problem of unable detection of ampicillin and sulfadimethoxine of MCH self-assembled passivation layers commonly used in SD-EAB in the prior art is solved in the invention.

Description

technical field [0001] The invention relates to a method for forming a self-assembled passivation layer for an ampicillin and sulfidesoxine electrochemical sensor and the electrochemical sensor, belonging to the technical field of biological analysis. Background technique [0002] Nucleic acid aptamer (Aptamer) is a DNA (deoxyribose nucleic acid) or RNA (ribonucleic acid) sequence obtained through in vitro screening, which can bind to a variety of target substances with high specificity and high selectivity. Compared with antibodies, it has the ability to artificially Synthesis, good stability, convenient chemical modification and engineering design and other advantages, so it has a good application prospect in the field of biosensors. [0003] Antibiotics play an extremely important role in the treatment of infectious diseases, but due to serious abuse in recent years, the problem of antibiotic residues in animal foods has become prominent. These residual antibiotics will ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
Inventor 娄新徽何苗丁小凡杨子华
Owner CAPITAL NORMAL UNIVERSITY
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