A method for preparing freeze-dried active placenta powder by water-soluble enzymatic hydrolysis

Abstract

The invention discloses a method for preparing freeze-dried active placenta powder through water solution enzymolysis. The method includes five technological steps including homogenizing, water solution, enzymolysis, freeze drying and ozone disinfection; water-soluble nutrient substances are extracted after placenta homogenizing, enzymatic products and the water-soluble substances are subjected to vacuum drying after stomach chymosin and chymotrypsin enzymolysis of insoluble substances, and finally, high-speed pulverization is achieved at a low temperature. The method has the advantages that low loss of placenta nutrient substances is guaranteed, separation and extraction efficiency is improved, the prepared active placenta powder is easy to digest and absorb, more than 98% of active substances can be maintained, and reliable scientific bases and technical measures are provided for producing health-care food by placentae.

Description

technical field [0001] The invention belongs to a method for preparing bioactive placenta freeze-dried powder by combining water-soluble extraction and enzymatic hydrolysis. It belongs to the research and development field of health food. Background technique [0002] Placenta, also known as "Ziheche", has a long history as a traditional Chinese medicine tonic in my country. Modern scientific research has found that placenta is rich in immune proteins, nutritional peptides, cytokines, terpenes and hormones, which can improve hematopoiesis. function, ovarian health care, promotion of lactation and beauty. Both fresh and dried placenta can be used as medicine. The "Compendium of Materia Medica" elaborates on its properties and medicinal effects: Ziheche "taste is sweet or salty", "nature is flat and warm", and it is home to the heart, lung and kidney meridians. , has the functions of nourishing blood, nourishing qi, replenishing essence, detoxifying, and nourishing blood. It ...

AI Technical Summary

Problems solved by technology

Existing methods for preparing placenta freeze-dried powder, such as "A Method for Separation and Extraction of Porcine Placenta" announced on February 12, 2014, only extract water-soluble substances without enzymatic hydrolysis, and lose the abundant collagen and other components in the placenta; Another example is the "Method for Extracting Sheep Placenta from Sheep Placenta Using Liquid Nitrogen Low-temperature Oxygen Exhaust" patent published on March 3, 2010 with the publication number 10165853A. The disclosed method is to add liquid nitrogen to the placenta, and the crushing temperature is 24- Enzymolysis of placental pulp at 25°C for 60 minutes at 40°C, then extraction with normal saline, filtration with inorganic membrane, and ultra-high pressure sterilization

The main disadvantages of this method are: 1) the crushing is carried out under liquid nitrogen, the conditions are harsh, the equipment investment is high, and the production cost is high; 2) the natural peptide active substances in the sheep placenta, such as water-soluble hormones, cytokines, small molecules, etc. After the ribonucleic acid is separated, it is enzymatically hydrolyzed, and the protease also hydrolyzes the above-mentioned active substances, destroying the above-mentioned peptide active substances, and the activity of the product is low; No. 201110438853.X "A Method for Preparing Sheep Placenta and Sheep Placenta Hydrolyzed Collagen Concentrate" adopts the ultrafiltration method with a molecular weight cut-off of 2000-10000Da to concentrate and mention, and loses a large amount of small molecule active substances

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