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Mosquito-killing bacillus thuringiensis floating agent and preparation method thereof

A technology of Bacillus thuringiensis and Bacillus thuringiensis, which is applied in the field of biological pesticides, can solve the problems of waste and frequent application of pesticides, and achieve the effects of reasonable formula, enhanced anti-ultraviolet radiation ability, and long residual effect in the field

Inactive Publication Date: 2015-08-26
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to control mosquito hazards more ideally, frequent application of pesticides is required, resulting in waste

Method used

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  • Mosquito-killing bacillus thuringiensis floating agent and preparation method thereof
  • Mosquito-killing bacillus thuringiensis floating agent and preparation method thereof

Examples

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Effect test

Embodiment 1

[0017] Embodiment 1: a kind of preparation method of mosquito-killing Bacillus thuringiensis preparation comprises the following steps:

[0018] (1) Take sodium alginate, Bacillus thuringiensis original powder, hollow glass microspheres, melanin, sodium benzoate, mouse feed and water, and prepare a mixed solution. The weight percentage of each raw material in the preparation is 0.8% and 3% respectively , 3.2%, 0.012%, 0.3%, 0.5%, and the rest of water;

[0019] (2) preparing an aqueous solution of calcium chloride with a concentration of 4.9% by weight;

[0020] (3) Use a 10mL sterile syringe to add the mixed solution in step (1) dropwise to the calcium chloride aqueous solution in step (2), and the droplets will form granules and solidify;

[0021] (4) The hardening time of the droplets in step (3) in the calcium chloride aqueous solution was 4.7 h. The calcium chloride aqueous solution was discarded, and the hardened granular microbeads were washed twice with distilled wate...

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Abstract

The invention relates to a mosquito-killing bacillus thuringiensis floating agent and a preparation method thereof. The preparation method comprises the steps of blending, calcium chloride aqueous solution preparation, floating agent preparation, hardening, and drying. According to the invention, sodium alginate is adopted as a carrier, and sodium chloride is adopted as a crosslinking agent. The added mosquito-killing bacillus thuringiensis original powder is an effective active component with high mosquito-killing activity. With hollow glass microspheres, a floating sustained-release effect is provided for the dosage form. Ultraviolet radiation is reduced, and a residual effect period is prolonged. With an ultraviolet protection additive which is melanin, the ultraviolet radiation resistance of bacillus thuringiensis is enhanced. With a lab rat feed, the floating agent is provided with an attractant effect and can lure larvae, such that the insecticidal effect is improved. Also, a sinking phenomenon of the dosage form left in water for a long time is avoided, such that the residual effect period is prolonged. The product is solid; the formula is reasonable; and insecticidal virulence is high. When in use, the product is simply spread onto the surface of pond or sewage with mosquito breeding. A controlling effect is good; and convenience is provided for development, popularization and application.

Description

technical field [0001] The invention relates to a biological pesticide preparation and a preparation method thereof, in particular to a mosquito-killing bacillus thuringiensis floating agent and a preparation method thereof, belonging to the technical field of biological pesticides. Background technique [0002] Bacillus thuringiensis ( Bacillus thuringiensis , Bt), is a Gram-positive bacterium, and its main feature is the formation of spores and the accompanying formation of insecticidal crystal proteins (Insecticidal Crystal Proteins, ICPs) with protein properties in the bacterium, also known as δ ~ endotoxin, the Crystal proteins play a major role in the insecticidal process of Bt as an insecticide against Diptera, Coleoptera, Hymenoptera, Homoptera, Lepidoptera, Orthoptera, Trichophaera, Liceoptera and Flea insects, trematodes, flagellates, nematodes, and acarids have strong poisonous effects, and have the advantages of not polluting the environment, harmles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/04A01P7/04
Inventor 张灵玲朱小丽陈慧成刘健楠徐卓吟郭雅洁
Owner FUJIAN AGRI & FORESTRY UNIV
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