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A kind of total alkaloid extract and application thereof

A technology for the total alkaloids of A. chinensis, which is applied in the directions of drug combination, organic chemistry, anti-tumor drugs, etc., can solve the problems of chemical constituents in C. The effect of high rate and simple process flow

Inactive Publication Date: 2017-03-22
HAINAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Preliminary studies have found that the ethanol extract of Fleur-de-Flax has good anti-HIV activity [He J.J, Park I.W, Chen G.Y. et al. Extracts of medicinal plant and uses therefore of. PCT Int. Appl. 2011, WO 2011014561A120110203. ], in addition, there are no research reports on the chemical constituents and biological activities of the trichomes at home and abroad.

Method used

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  • A kind of total alkaloid extract and application thereof
  • A kind of total alkaloid extract and application thereof

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preparation example Construction

[0022] The steps of the preparation method of the total alkaloid extract of the branches and leaves of the hawk's claw are as follows:

[0023] 1) Soak the crushed dry powder of the branches and leaves of P. tomentosa (through a 20-80 mesh sieve) with 0.5-2% acetic acid, hydrochloric acid, sulfuric acid or tartaric acid at room temperature or ultrasonic extraction, and the extraction time is 1 to 7 days. The ratio of solid to liquid is 1:1~1:3.

[0024]2) Merge the water extracts of the follic acid of the Fleur-de-Flavonica branch, filter and wash with saturated Na 2 CO 3 Alkalinize the solution or 20% ammonia water, adjust the pH value to 10, add ethyl acetate to extract, and recover under reduced pressure to obtain the ethyl acetate part, that is, the total alkaloids in the branches and leaves of the hawk's claw.

[0025] 3) Systematic research on the chemical composition of the ethyl acetate part: the ethyl acetate part was separated by reverse-phase silica gel column chr...

example 1

[0028] Example 1, the preparation method of the total alkaloid extract of the branches and leaves of the hairy leaf eagle's claw

[0029] The branches and leaves (40.0Kg) of Pleurotus chinensis (40.0Kg) were dried in the shade, crushed and sieved, soaked at room temperature with 0.5%-2% acetic acid, hydrochloric acid, sulfuric acid or tartaric acid for 3 times, each time for 3 days, the ratio of solid to liquid was 1:2, and the combined acid The water extract was filtered with saturated Na 2 CO 3 Alkaline the solution or 20% ammonia water, adjust the pH value to 10, add ethyl acetate for extraction; recover under reduced pressure to obtain the ethyl acetate fraction (128.0 g), that is, the total alkaloids in the branches and leaves of Arachnina tomentosa.

[0030] After repeated reverse-phase silica gel column chromatography and Sephadex column chromatography on the extraction part of ethyl acetate, 12 alkaloids were separated and identified, including 4 aporphines, 2 benzyli...

Embodiment 2

[0032] Example 2: Research on the antitumor activity of the total alkaloid extracts from the branches and leaves of S.

[0033] 1. Experimental method: Four common tumor cell lines K562, SPCA-1, SGC-7901 and BEL-7402 were used in RPMI-1640 medium containing 10% calf serum at 37°C, 5% CO 2 cultured in an incubator. The MTT method was used to carry out the cell proliferation inhibition test. The main operation was: take the tumor cell line in the logarithmic growth phase, digest it with 0.25% trypsin, and prepare 5×10 RPMI-1640 culture solution with 10% newborn calf serum 4 cells / mL of cell suspension, inoculated in 96-well plate, inoculated 180 μL per well. At 37°C, 5% CO 2 Cultivate for 8-10 hours under saturated humidity conditions, and when they adhere to the wall, add sample solution prepared with PBS to each well so that the final concentrations of the samples are 0.1, 1, and 10 μg / mL, respectively. For each concentration, 3 wells were paralleled, and after 44 hours of ...

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Abstract

The invention discloses an artabotrys pilosus total alkaloid extract, which is prepared by the following steps: taking dry powder of artabotrys pilosus branches and leaves, carrying out cold soaking extraction with acid water, and mixing acid water extract liquid; filtering, and alkalifying with alkaline water; adding ethyl acetate, extracting, recovering the ethyl acetate in vacuum to obtain an ethyl acetate extract part, namely the total alkaloid part of the artabotrys pilosus branches and leaves, wherein the part mainly comprises 12 alkaloids, including four aporphinoid alkaloids, two benzylisoquinoline alkaloids, two original aporphinoid alkaloids, two morphinane alkaloids and two protoberberine alkaloids. The prepared total alkaloid extract is high in yield, simple in technological process, low in cost and suitable for relatively large-scale preparation, has good antitumor activity, and can be applied to treatment of various tumorous diseases independently or together with other anti-tumor medicines to treat various tumorous diseases.

Description

technical field [0001] The invention belongs to the field of natural medicines, and relates to a total alkaloid extract and its application, in particular to a total alkaloid extract of Pleurotus pachyrhiza and its application in antitumor drugs. Background technique [0002] Annonaceae (Annonaceae) Artabotrys plants are usually climbing shrubs. There are about 100 species in the world, distributed in tropical and subtropical regions. There are 4 species in my country, namely A.pilosus Merr .etChun), A.hainanensis R.E.Fries, A.hongkongensis Hance and A.hexapetalus (L.f.) Bhandari, distributed from southwest to southeast, where The flora and narrow petals are endemic to Hainan [Editorial Committee of Flora of China, Chinese Academy of Sciences. Flora of China. Vol. 30. Science Press. Beijing: 1979, p121-128.]. Plants of the genus Eagle's claw have been used as medicinal plants in the folk for a long time. They have the functions of clearing away heat and detoxifying, reducing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D221/18C07D491/056C07D217/20C07D221/20C07D221/28C07D455/03A61P35/00
CPCC07D217/20C07D221/18C07D221/20C07D221/28C07D455/03C07D491/056
Inventor 刘艳萍付艳辉陈光英韩长日宋小平李小宝
Owner HAINAN NORMAL UNIV
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