Method for evaluating activity of aspergillus niger mouldy bran spore

Abstract

The invention discloses a method for evaluating the activity of aspergillus niger mouldy bran spore. The method comprises the following steps: (1) washing cultured aspergillus niger mouldy bran spore with a phosphate buffer liquid, and filtering, thereby obtaining aspergillus niger mouldy bran spore suspension; (2) adding 0.055% (v / v) of Tween 80, and performing ultrasonic treatment, thereby obtaining uniformly dispersed monospore suspension, and adjusting the concentration of the monospore suspension to be 10<6>-10<7> pieces / mL; (3) preparing a 8-14mg / L methylene blue solution from the phosphate buffer liquid; (4) uniformly mixing the solutions of the steps (2) and (3) according to a ratio (v / v) of 1:1, sealing to react for 420 seconds, and scanning an absorbancy-time curve at the wavelength of 663nm; (5) testing the primary absorbancy and the absorbancy after the reaction is completed, of a reaction system, and representing the activity of cells according to the change of the absorbancy. By adopting the method, the culture time of mature seeds can be effectively shortened, the raw material utilization rate can be increased, and the technique is also applicable to evaluation on the activity of other microorganism spore.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for evaluating the activity of Aspergillus niger aspergillus gluten spores. Background technique [0002] Citric acid (Citric Acid) is an important organic acid with multiple functions, widely used in food, medicine, chemical industry and other fields; it is the edible organic acid with the largest output and consumption in the world, with a global output of more than 1.7 million tons. At the same time, citric acid has excellent biological characteristics, and has great application potential in emerging industries such as biopolymerization, drug delivery, and cell culture, and its demand is growing at a rate of 5% per year. [0003] Aspergillus niger is an important industrial microorganism. It is favored by the citric acid industry because of its safe enzyme system, various types of carbon sources, strong acid production ability, and strong acid resistance ability. In indu...

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Problems solved by technology

However, these detection methods have disadvantages such as long operation process, reaction reagents need to be prepared immediately, MTT is carcinogenic, and the equipment is expensive.

[0005] Aspergillus bran spores are special dormant bodies of Aspergillus niger. The spore wall is thick and has poor permeability, making it difficult for foreign substances to intervene. At the same time, the force between spores is strong, and the spores adhere together, making it difficult to form uniformly dispersed single spores; Therefore, there are few reports about the research on the viability of Aspergillus niger gluten spores

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