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Preparation methods of prodigiosin and biological stain as well as application of biological stain in fabric

A prodigiosin and dye technology, applied in the field of biological dyes, can solve problems such as waste and environmental pollution, and achieve the effects of convenient preparation, simple extraction process and good antibacterial properties

Inactive Publication Date: 2015-07-22
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We use the main products (such as starch, alcohol, monosodium glutamate) and discard other ingredients, which not only pollutes the environment but also wastes resources

Method used

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  • Preparation methods of prodigiosin and biological stain as well as application of biological stain in fabric
  • Preparation methods of prodigiosin and biological stain as well as application of biological stain in fabric
  • Preparation methods of prodigiosin and biological stain as well as application of biological stain in fabric

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Activation of the original strain

[0045] According to the composition of the seed medium, weigh 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, and 15g of agar, put them into a 1000mL beaker, add water to 1000mL, heat and stir to dissolve, and use 1mol / L of HCl and 1mol / L of NaOH Adjust the pH of the medium so that the pH is 7.4-7.6. Then it was divided into 250mL shake flasks, sterilized at 121°C for 20min with a sterilizer, and finally poured into a plate. After the plate is cooled, under aseptic conditions, use an inoculation needle to pick up the strains in the glycerol tube to scratch the plate, place it in a biochemical incubator, and incubate at 37°C for 12 hours to obtain activated Serratia, Staphylococcus aureus, Bacillus subtilis, put it in the refrigerator and save it for later use;

[0046] (2) Obtain inactivated Staphylococcus aureus and Bacillus subtilis

[0047] Weigh peptone 13.0g, glycerol 20.0g, MgSO 4 Add 1.2g, NaCl 5.0g, and Gly 2.0g ...

Embodiment 2

[0074] (1) Activation of the original strain

[0075] According to the composition of the seed medium, weigh 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, and 15g of agar, put them into a 1000mL beaker, add water to 1000mL, heat and stir to dissolve, and use 1mol / L of HCl and 1mol / L of NaOH Adjust the pH of the medium so that the pH is 7.4-7.6. Then it was divided into 250mL shake flasks, sterilized at 121°C for 20min with a sterilizer, and finally poured into a plate. After the plate is cooled, under aseptic conditions, use an inoculation needle to pick up the strains in the glycerol tube to scratch the plate, place it in a biochemical incubator, and incubate at 37°C for 12 hours to obtain activated Serratia, Staphylococcus aureus, Bacillus subtilis, put it in the refrigerator and save it for later use;

[0076] (2) Obtain inactivated Staphylococcus aureus and Bacillus subtilis

[0077] Weigh peptone 13.0g, glycerol 20.0g, MgSO 4 Add 1.2g, NaCl 5.0g, and Gly 2.0g ...

Embodiment 3

[0085] (1) Activation of the original strain

[0086]According to the composition of the seed medium, weigh 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, and 15g of agar, put them into a 1000mL beaker, add water to 1000mL, heat and stir to dissolve, and use 1mol / L of HCl and 1mol / L of NaOH Adjust the pH of the medium so that the pH is 7.4-7.6. Then it was divided into 250mL shake flasks, sterilized at 121°C for 20min with a sterilizer, and finally poured into a plate. After the plate is cooled, under aseptic conditions, use an inoculation needle to pick up the strains in the glycerol tube to scratch the plate, place it in a biochemical incubator, and incubate at 37°C for 12 hours to obtain activated Serratia, Staphylococcus aureus, Bacillus subtilis, put it in the refrigerator and save it for later use;

[0087] (2) Obtain inactivated Staphylococcus aureus and Bacillus subtilis

[0088] Weigh peptone 13.0g, glycerol 20.0g, MgSO 4 Add 1.2g, NaCl 5.0g, and Gly 2.0g i...

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Abstract

The invention discloses preparation methods of prodigiosin and prodigiosin-containing biological stain as well as an application of the prodigiosin-containing biological stain in fabric. The preparation method of the prodigiosin comprises the following steps: (1), red pigment stains with the preservation number of CGMCC (China General Microbiological Culture Collection Center) 4074 are cultured in a microbial culture solution, and prodigiosin-containing fermentation broth is obtained; (2), the prodigiosin-containing fermentation broth is subjected to centrifugation and supernatant removal to obtain a precipitate, an organic solvent is added to the precipitate, the mixture is subjected to centrifugation, the organic solvent in supernatant is evaporated, and prodigiosin solids are obtained. The special microbial culture solution can improve the output of the prodigiosin, and industrial waste protein products can be adopted as raw materials to replace imported peptone used for fermentation production of the prodigiosin; according to a preferable technical scheme, the output of the prodigiosin can be further increased with adoption of mixed culture, and the antibacterial property is improved.

Description

technical field [0001] The invention relates to the field of biological dyes, in particular to a preparation method of prodigiosin and a prodigiosin-containing biological dye and an application of the prodigiosin-containing biological dye in fabrics. Background technique [0002] In recent years, due to the influence of environmental problems and energy problems, people have begun to pursue environmentally friendly, sustainable, and harmless methods to prepare dyes. The development of natural dyes is the general trend of the development of applied dyes in the world. Natural dyes refer to dyes obtained from plants, animals or mineral resources with little or no chemical processing. Many natural dyes are safe, reliable, natural in color, and close to natural substances. And other advantages, natural, multi-functional is the development trend of dyes. With the development of biological science, the use of biotechnology to produce natural dyes has become a widely developed fiel...

Claims

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Application Information

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IPC IPC(8): C12P17/16C09B61/00D06P1/34C12R1/43
Inventor 白林静陈顺丽黄一峰刘晓侠沈加加王玉洁
Owner JIAXING UNIV
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