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Application of radix bupleuri and herba houttuyniae in preparation of medicines for prevention and treatment of ionising radiation damage

A technology of ionizing radiation and Houttuynia cordata, which is applied in the field of medicine, can solve the problems of short half-life, limiting the wide application of radiotherapy, and the application has not been reported yet.

Inactive Publication Date: 2015-07-22
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] On the other hand, the incidence of malignant tumors and the number of patients have been on the rise in recent years. Radiation therapy, as one of the main treatment methods, plays an indispensable role, but high-dose radiation exposure will inevitably lead to normal Acute radiation damage to tissues, organs and even the whole body, the side effects caused by radiation damage seriously limit the wide application of radiation therapy in tumor treatment, and also significantly affect the curative effect of radiation therapy and the quality of life of cancer patients after treatment
[0005] Drugs related to the treatment of radiation damage currently on the market mainly include: sulfur-containing compounds, hormones, cytokines, and Chinese herbal medicines, etc., which have their own inherent defects: for example, sulfur-containing compounds generally have relatively large side effects, and amifostine (also known as Amifostine ) as the representative of this type of compound is currently recognized as the compound with the best protective effect, and is the first selective broad-spectrum cytoprotective agent passed by the international regulatory agency, but the half-life is extremely short (7 minutes) and expensive (domestic The medical market price is 400-500 yuan / branch) which limits its application; while the prevention and treatment of hormonal drugs to radiation damage is mainly to bone marrow nucleated cells, hematopoietic stem cells and progenitor cells, and the impact of such drugs on sexual organs and reproductive system It limits its wide use; cytokine drugs such as interleukins and colony-stimulating factor drugs can alleviate and treat the damage to the bone marrow hematopoietic function system caused by radiation, but its radiation protection effect is closely related to the time of administration (used for prevention and In the medical practice of rescue and treatment, such drugs require high levels of medical testing and attention), have obvious inflammatory effects, are expensive, and are difficult to store at room temperature; anti-radiation components of Chinese herbal medicines mainly include phenols, polysaccharides, natural Flavonoids have the characteristics of unclear active ingredients and low toxicity. After years of research, there are no similar drugs on the market or to be listed
[0008] So far, the application of Bupleurum extract, Houttuynia cordata extract, and the composition of the two in the preparation of auxiliary drugs for the prevention and treatment of ionizing radiation damage and tumor radiation therapy has not been reported

Method used

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  • Application of radix bupleuri and herba houttuyniae in preparation of medicines for prevention and treatment of ionising radiation damage
  • Application of radix bupleuri and herba houttuyniae in preparation of medicines for prevention and treatment of ionising radiation damage
  • Application of radix bupleuri and herba houttuyniae in preparation of medicines for prevention and treatment of ionising radiation damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Treatment of Radiation Injury Caused by Single Lethal Dose (13Gyx-ray / γ-ray) Irradiation

[0022] The Bupleurum bupleuri extract can be prepared by referring to the published invention patent instructions or related documents, or can be purchased from the pharmaceutical market. The administration method is intraperitoneal injection, and the dosage is 200-400mg / Kg body weight (rat). Houttuynia cordata injection, Hebei Shenwei Pharmaceutical Co., Ltd., Z13020888, batch number 13041222. The administration method is intraperitoneal injection, and the dosage is 100-200 μl / rat.

[0023] X-ray irradiation device X-Rad RS-2000 irradiator, voltage 160KV, band ray energy 50KeV, dose rate 1.01Gy / min.

[0024] Gamma ray irradiation device 137 Cs irradiator, dose rate 0.8Gy / min.

[0025] C57BL / 6 mice, half male and half female, weighing 18-20g, certificate number SCXK (Beijing) 2012-0001, grouped as follows: no irradiation group, irradiation plus normal saline group, irradiation p...

Embodiment 2

[0030] Oral administration of Bupleurum extract and Houttuynia cordata extract for the treatment of radiation damage caused by a single sublethal dose (9Gy γ-ray) irradiation

[0031] Ethanol extraction process of Houttuynia cordata: Grind and pulverize 10 g of dried Houttuynia cordata, put it in a 250ml flask, add a 70% ethanol solution according to the ratio of solid to liquid (1:20 (g / ml), put it in a water bath at 80°C Reflux leaching in the pot, reflux twice for 2 hours each time, then centrifuge the extract at 4000r / min for 20min, and concentrate the supernatant with a rotary evaporator.

[0032] Bupleurum bupleuri extract can be prepared with reference to the published invention patent specifications or related documents, and the dosage is 1g / Kg body weight (mouse). Houttuynia cordata ethanol extract, dosage is 1.575g / Kg body weight (mouse), above-mentioned dosage is raw material drug weight. Animal information and irradiation device are the same as those in Example 1....

Embodiment 3

[0038] Hematopoietic protection against radiation damage caused by low-dose (5Gy γ-ray) irradiation

[0039] Drug information, animal information, irradiation device, and administration method are the same as those in Example 1.

[0040] The irradiation dose is 5Gy.

[0041] The administration was continued for 7 days after the irradiation, and the animals were sacrificed two days after the withdrawal of the administration for observation, and samples were taken for detection.

[0042] Detection indicators: body weight, peripheral blood count, unilateral femur count, granulocyte-macrophage colony-forming unit, spleen index, and thymus index.

[0043] See the experimental results image 3 , the combined administration of Bupleurum and Houttuynia can significantly alleviate the hematopoietic system damage in animals caused by low-dose irradiation.

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Abstract

The present invention relates to the field of medicines, is named as application of radix bupleuri and herba houttuyniae in preparation of medicines for prevention and treatment of ionising radiation damage, and discloses application a combination of two pure traditional Chinese medicine preparations of bupleurum chinense or bupleurum scorzonerifolium total extract and herba houttuyniae extract in preparation of medicines for prevention and treatment of ionising radiation damage. The invention discloses that the radix bupleuri extract and the herba houttuyniae extract can play a radiation protective effect in cell and living animal levels, and has a protective effect in a wide dose range comprising lethal dose, sub-lethal dose and low dose radiation, the combination of the two active extracts is better than single use of the extracts, the combination is low in cost and wide in sources, and can be used for preparation of the medicines for prevention and treatment of ionising radiation-caused relative damages and diseases.

Description

[0001] Involved areas [0002] The invention relates to the field of medicine, in particular, the active ingredients extracted from Bupleurum chinensis or Bupleurum angustifolia and the active ingredients extracted from Houttuynia cordata are used in the preparation of related diseases caused by ionizing radiation. Injury care and disease treatment applications. Background technique [0003] With the vigorous development of the global nuclear industry, nuclear technology has been widely used in various fields such as nuclear power plants, aerospace, national defense, and biomedicine. Human beings are more likely to be exposed to ionizing radiation and cause damage. The hidden dangers of nuclear war and nuclear terrorism, the protection and treatment of body damage caused by ionizing radiation (referred to as radiation damage) are receiving more and more attention. [0004] On the other hand, the incidence of malignant tumors and the number of patients have been on the rise in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/78A61P17/16A61P35/00A61P7/00
Inventor 樊赛军贺欣
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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