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Technology of using ocean phaffiarodozyma for producing feed additive astaxanthin in fermentation mode

A feed additive, yeast technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of no production scale, low production efficiency, low technical level, etc., to reduce production costs, improve production efficiency, and improve production efficiency. effect of prevention

Inactive Publication Date: 2015-07-15
CHANGYI MINGXING FEED CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In Yunnan Province of my country, an astaxanthin production plant with green algae technology was once built, but was forced to resell it because of low production efficiency and high cost.
The yeast astaxanthin production line built on the southeast coast also failed to form a real production scale due to low technical level and single product. As a result, the country was forced to import a large amount of Roche's synthetic astaxanthin, and paid a high price.

Method used

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  • Technology of using ocean phaffiarodozyma for producing feed additive astaxanthin in fermentation mode
  • Technology of using ocean phaffiarodozyma for producing feed additive astaxanthin in fermentation mode
  • Technology of using ocean phaffiarodozyma for producing feed additive astaxanthin in fermentation mode

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Embodiment 1

[0040] A process for producing feed additive astaxanthin by fermenting marine rhodotorula, comprising the following steps:

[0041] (1) Seed culture of Rhodotorula marinae: prepare liquid culture medium and divide it into 1000ml Erlenmeyer flasks, adjust pH=5.0, sterilize at high pressure 137kpa, high temperature 121℃ for 20min, and fill liquid volume to 400ml. After cooling, the strains were inoculated on an aseptic operating table, and then cultured in a shaking table at 30°C for 40 hours before being used for later use. The rotating speed of the shaking table was 160r / min.

[0042] Medium formula: glucose: 10g; yeast extract powder: 3g; peptone: 5g; wort juice: 3g; water: 100ml.

[0043] (2) First-level seed culture of Rhodotorula marinae: Inoculate the seeds of Rhodotorula marinae cultured for 40 hours into a 100L sterilized seed fermenter, with a liquid volume of 60%, an inoculum volume of 10%, a culture temperature of 30°C, and ventilation Cultivate with stirring for 24...

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Abstract

The invention discloses a technology of using ocean phaffiarodozyma for producing feed additive astaxanthin in a fermentation mode. The technology includes the steps that glucose, yeast extract powder and the like serve as main raw materials to carry out liquid state submerged fermentation cultivation of special type yeast strains; fermenting-ripening astaxanthina saccharomycetes liquid is pumped into a horizontal low speed centrifuge to be centrifuged; astaxanthina saccharomycetes residue suspension liquid obtained through centrifugation is squeezed in a box pressure filter until the solid content is about 30%, the suspension liquid is moved into a fluidized bed and dried at low temperature till the moisture content is reduced to below 5%, and then the feed additive astaxanthin with the astaxanthin content of 10% is obtained through pulverizing and filtering. The technology has the advantages that as the astaxanthin production capacity strains are matched with a highly shared and complementary product extracting and purification technology, two most important natural antioxidant chiral drug intermediates and the feed additive are simultaneously obtained through one production line, the production cost is reduced, and the productivity benefits of the astaxanthin are increased.

Description

technical field [0001] The invention relates to a production process of astaxanthin, in particular to a process for producing feed additive astaxanthin by fermenting marine rhodotorula, belonging to the field of astaxanthin production. Background technique [0002] Astaxanthin / astacin / astacean is the main carotenoid component in aquatic organisms in nature, which is of great significance to the life activities of aquatic organisms. Like most animals, humans cannot synthesize carotenoids in the body, and can only obtain them through food intake. For example, aquatic products, such as shrimp, crab and some cold-water fish contain astaxanthin, which becomes a supplement for people. Animal sources of carotenoids. Astaxanthin is an oxidized carotene. Studies have shown that the antioxidant capacity of astaxanthin is 500 times that of vitamin E and more than 11 times that of carotene. It has obvious antioxidant and elimination of active singlet oxygen. . [0003] The final prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 王福强邱彦国王华峰邹希成臧智强张艾青高金明谢虹辛国升单庆美苏成文李舫姜八一
Owner CHANGYI MINGXING FEED CO LTD
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