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Application of sesquiterpene lactone compounds in the preparation of anti-influenza virus drugs

An ester compound, anti-influenza virus technology, applied in the field of medicine, achieves the effects of easy availability of raw materials, good anti-influenza virus activity, and simple preparation method

Inactive Publication Date: 2017-06-23
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most influenza viruses are now resistant to amantadine and oseltamivir drugs

Method used

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  • Application of sesquiterpene lactone compounds in the preparation of anti-influenza virus drugs
  • Application of sesquiterpene lactone compounds in the preparation of anti-influenza virus drugs
  • Application of sesquiterpene lactone compounds in the preparation of anti-influenza virus drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Grind 300g of dried goose-free grass, pass through a 80-mesh sieve, and place in supercritical CO 2 In the extractor, the extraction pressure is 20 MPa, the extraction temperature is 30°C, the flow rate is 15 liters / hour, and the extraction time is 2 hours, and 14.8 g of supercritical carbon dioxide extract of goose and herbivorous are obtained;

[0020] Dissolve the supercritical carbon dioxide extract of Goose Bubiograss in ethyl acetate, mix the sample with 12g of silica gel (100-200 mesh), and evaporate to dryness under reduced pressure to obtain the sample; take another 90g of silica gel (200-300 mesh) for dry packing Column (inner diameter × length = 3.5 × 50cm), put the sample on the column, then cover with a layer of silica gel, first elute with petroleum ether, and then use petroleum ether and ethyl acetate (volume ratio: 7.5:1.5) Mixed solvent elution, collecting the mixed solvent eluted part, concentrating and drying to obtain 2.4 g of the mixed solvent elute...

Embodiment 2

[0023] Example 2 Anti-influenza A virus activity test of sesquiterpene lactones

[0024] Take a 96-well cell culture plate covered with a monolayer of MDCK cells, discard the supernatant, wash twice with PBS, add 100TCID 50 The virus solution was 100 μL / well, and was adsorbed for 1 h, and then 100 μL of the drug solution diluted with MEM was added to each well. In addition, a cell control group, a virus control group, and a blank control group were set up, and ribavirin was used as a positive control drug. Placed at 37°C, saturated humidity, 5% CO 2 Incubate in the incubator. After 48 h, the old solution in the 96-well plate was discarded, and the original solution of CCK8 was diluted ten times and added to the tested wells at 100 µL per well. After incubation in the incubator for 1 h, the absorbance was measured at 450 nm with a microplate reader. IC 50 The calculation method is as follows: cell survival rate = (OD value of the drug-dosing well - OD value of the virus con...

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PUM

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Abstract

The invention belongs to the field of medicines, and specifically discloses applications of sesquiterpene lactone compounds in the preparation of anti-influenza virus drugs. The anti-influenza virus drugs contain sesquiterpene lactone compounds with an effective dose and pharmaceutically-acceptable carriers. The invention finds that sesquiterpene lactone compounds have an anti-influenza virus effect, and novel compounds are provided for the preparation of anti-influenza virus drugs. The provided sesquiterpene lactone compounds have a very good anti-influenza virus activity, and the experiment results show that the anti-influenza virus IC50 value of the sesquiterpene lactone compounds is smaller than that of a positive reference drug ribavirin.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of sesquiterpene lactone compounds in the preparation of anti-influenza virus drugs. Background technique [0002] Influenza viruses are a major threat to human health. The respiratory disease caused by influenza virus is called influenza (flu), which has the characteristics of wide prevalence, strong infectivity and high incidence. According to statistics, there are 600-1200 million influenza cases in the world every year, of which 3-5 million are severe influenza cases, 250-500 thousand people die, and the case fatality rate of severe influenza can reach 10%. [0003] Due to the variation of influenza virus antigens, conventional vaccines are still unable to effectively prevent influenza outbreaks and epidemics. Therefore, research on anti-influenza virus drugs is of great significance in the treatment of influenza. Anti-influenza drugs currently on the market can be r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365C07D307/93A61P31/16
Inventor 李药兰王国才和君李满妹张晓丽张玉波李国强
Owner JINAN UNIVERSITY
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