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Application of mangiferin in preparing anti-influenza virus medicament

An anti-influenza virus and mangiferin technology, applied in the field of mangiferin, can solve problems such as neurotoxicity, ineffectiveness of influenza B virus, and influenza virus mutation

Inactive Publication Date: 2009-06-24
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extensive clinical application of existing drugs, the influenza virus has mutated, resulting in varying degrees of resistance to these drugs, and ion channel blockers have defects such as ineffectiveness against influenza B virus and neurotoxicity.

Method used

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  • Application of mangiferin in preparing anti-influenza virus medicament
  • Application of mangiferin in preparing anti-influenza virus medicament
  • Application of mangiferin in preparing anti-influenza virus medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Mangiferin was purchased from the National Institute for Standardization, with a purity of 99.5%.

[0013] Refer to the national standards of the People's Republic of China GB15193.3-94, GB15193.5-94, GB15193.8-94, GB15193.7-94, GB15193.4-94, GB15193.14-94 and GB15193.13-94, carry out large-scale Mouse acute toxicity test, mouse acute toxicity test, mouse bone marrow micronucleus test (male), mouse bone marrow micronucleus test (female), mouse sperm abnormality test, mouse testis chromosomal aberration test, Ames test, rat system Teratogenicity test and 30-day feeding experiment of rats, the results showed that the LD50 of large and small mice was >30.0g / kg; the mice took mangiferin (500mg / kg) orally, once a day, and took it for 15 days, and observed it in an awake state. The psycho-nervous system, cardiovascular system and respiratory system showed no abnormalities.

Embodiment 2

[0015] Anti-influenza virus effect of mangiferin

[0016] 1. Study on anti-influenza virus effect of mangiferin in vitro

[0017] 1. Inhibitory effect of mangiferin on influenza virus in MDCK cell culture

[0018] (1) Cell culture: Add 0.25% trypsin (prepared by Hanks solution) to a culture flask full of MDCK cells, digest at 37°C for 5 minutes, add DMEM culture medium (containing 10% fetal bovine serum, 1% glutamine 3%) Pipetting, 1:3 subculture, 2-3d overgrowth, add cell counting board for counting, configure to 10 5 cells / ml, inoculate cell culture plate, 0.2ml per well of 96-well plate, 1ml per well of 24-well plate, 37°C, 5% CO 2 After culturing for 24 hours, the experiments were performed after the cells grew into a monolayer.

[0019] (2) Cytotoxicity test of drugs: MDCK cells susceptible to influenza virus were digested with 0.25% trypsin 1ml and Versene digestion solution 4ml respectively, then added growth medium, blown off, mixed, 100μl / well was inoculated in 96-...

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PUM

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Abstract

The invention discloses application of mangiferin in preparing an anti-influenza medicine. As proved by experiments, the mangiferin has anti-influenza function.

Description

technical field [0001] The present invention relates to the use of mangiferin, in particular to the application of mangiferin in the preparation of anti-influenza virus drugs. Background technique [0002] Many viral diseases seriously threaten the safety of human life. For example, influenza virus (Influenza Virus) belongs to the Orthomyxoviridae family, which is an enveloped RNA virus, including human influenza virus and livestock such as pigs and horses, as well as the armor of poultry such as chickens and birds. influenza virus. Human influenza virus can be divided into three types according to the antigenicity of nucleoprotein, A, B, and C, and its antigenicity is prone to variation. Human influenza is an acute upper respiratory infectious disease caused by influenza virus, which has the characteristics of high infectivity, rapid spread, and easy occurrence of epidemics. This is due to the particularity that the virus can only grow in living cells and the rapid variat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P31/12A61P11/00
Inventor 李君文庞佶郭金鹏王新为金敏谌志强
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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