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Novel calcium enhancer based on poorly-soluble calcium and food-borne protein complexes as well as preparation method and characterization method thereof

An insoluble, complex technology, applied in food preparation, food science, color/spectral property measurement, etc., can solve the problems of easy precipitation, poor solubility, poor solubility, etc., and achieve simple and safe process, not easy to migrate, not easy to precipitate. Effect

Inactive Publication Date: 2015-07-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing protein liquid or protein powder products, including high-calcium milk, formula milk powder, protein powder, protein bars, etc., insoluble calcium supplements still cause the problem of unstable product shelf life, such as easy precipitation, Poor solubility, migration of calcium supplement particles makes the taste bad, etc.

Method used

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  • Novel calcium enhancer based on poorly-soluble calcium and food-borne protein complexes as well as preparation method and characterization method thereof
  • Novel calcium enhancer based on poorly-soluble calcium and food-borne protein complexes as well as preparation method and characterization method thereof
  • Novel calcium enhancer based on poorly-soluble calcium and food-borne protein complexes as well as preparation method and characterization method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Dispersion: Prepare whey protein isolate (WPI) into a 10mg / mL solution, accurately weigh 20mg of food-grade tricalcium phosphate (TCP) and disperse in 0.5mL ultrapure water to form a suspension;

[0033] (2) Mixing: at room temperature, add 0.5mL of 10mg / mL whey protein isolate solution into 0.5mL tricalcium phosphate suspension (when no pH adjustment is performed, the pH of the mixed solution is detected to be ~7.0), and mix the two evenly , cultivated on a rotary incubator for 4 hours;

[0034] (3) Separation: After the above reaction is completed, the mixed solution is centrifuged (4000r / min, 10min), the supernatant and the precipitate are separated, the precipitate is redispersed in water, and spray-dried to obtain tricalcium phosphate compounded with whey protein isolate;

[0035] (4) Separation of staining and laser confocal imaging: after step (2), centrifuge the mixture (4000r / min, 10min), separate the supernatant and precipitate, rinse the precipitate and r...

Embodiment 2

[0039] (1) Dispersion: Prepare sodium caseinate (SC) into a 2 mg / mL solution, and accurately weigh 100 mg of food-grade calcium carbonate (CaCO 3 ) is dispersed in 2.5mL ultrapure water to form a suspension;

[0040] (2) Mixing: at room temperature, add 2.5mL of 10mg / mL sodium caseinate solution into 2.5mL of calcium carbonate suspension (when no pH adjustment is performed, the pH of the mixed solution is detected to be ~9.0), mix the two evenly, Spin culture on a rotary incubator for 2 h.

[0041] (3) Separation: After the above reaction is completed, the mixed solution is centrifuged (4000r / min, 10min), the supernatant and the precipitate are separated, the precipitate is redispersed in water, and spray-dried to obtain calcium carbonate compounded with sodium caseinate (CaCO 3 -SC).

[0042] (4) Separation of staining and laser confocal imaging: after step (2), centrifuge the mixture (4000r / min, 10min), separate the supernatant and precipitate, rinse the precipitate and re...

Embodiment 3

[0046] (1) Dispersion: Prepare milk protein concentrate (MPC) into a 10mg / mL solution, and accurately weigh 100mg of food-grade calcium carbonate (CaCO 3 ) and tricalcium phosphate (TCP) were dispersed in 2.5mL ultrapure water to form a suspension;

[0047] (2) Mixing: at room temperature, add 2.5mL of 10mg / mL MPC to 2.5mL of CaCO 3 and TCP suspension, mix well, and the control group (100mg CaCO 3 and 100mg TCP respectively dispersed in 5mL ultrapure water) together on a rotary incubator for 4 hours;

[0048] (3) Separation: After the above reaction is completed, the mixed solution is centrifuged (4000r / min, 10min), the supernatant and the precipitate are separated, the precipitate is redispersed in water, and spray-dried to obtain calcium carbonate (CaCO3) compounded with milk protein concentrate. 3 -MPC) and tricalcium phosphate (TCP-MPC).

[0049] (4) Separation of staining and laser confocal imaging: after step (2), centrifuge the mixture (4000r / min, 10min), separate th...

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Abstract

The invention discloses a novel calcium enhancer (poorly-soluble-protein complex) based on poorly-soluble calcium and food-borne protein complexes as well as a preparation method and a characterization method thereof, and a manufactured food exogenous calcium additive with high stability in liquid-state and solid-state protein products. According to the invention, hydrophilic and stable food-borne proteins and an insoluble calcium salt are combined to prepare a functional food, the food-borne proteins and food-grade poorly-soluble calcium are taken as raw materials, and the novel calcium enhancer is prepared by adopting an aqueous solution compounding method. The method disclosed by the invention is simple and safe in process and suitable for industrial production. According to the method, the problems that the taste of the product is poor, the preparation cost is high and the like because an extra stabilizer is added to stabilize the insoluble calcium salt are solved. Compared with the traditional poorly-soluble calcium, the prepared novel calcium enhancer based on food-borne protein poorly-soluble calcium has the advantage that the stability of the novel calcium enhancer is obviously improved in the liquid protein, protein powder or protein bar products.

Description

technical field [0001] The present invention relates to a novel calcium enhancer (insoluble calcium-protein complex) based on insoluble calcium and food-derived protein complex and its preparation and characterization method. Background technique [0002] The importance of calcium supplementation has been valued by more and more people, which is inseparable from the importance of calcium in the human body. 99% of calcium in the human body constitutes bones and teeth, supporting our life, and the remaining 1% exists in the soft tissue, extracellular fluid and blood of the human body, and plays an important physiological regulation function. According to the health survey of Chinese residents, the average daily calcium intake of Chinese residents is far lower than the daily recommended intake of 800-1000mg by the Chinese Nutrition Society. Therefore, strengthening calcium supplementation has become a part of people's lives. The product is the first choice for people to supple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/304A23L1/305G01N21/64G01N21/31A23L33/16A23L33/19
Inventor 周鹏胡锦华文丽杰
Owner JIANGNAN UNIV
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