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Milk protein detection method

A milk protein and detection method technology, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as regression mathematical models that have not been seen, and achieve the effects of assisting in the identification of ammonium salt adulteration, simple operation, and high detection efficiency

Inactive Publication Date: 2015-06-24
TARIM UNIV
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Problems solved by technology

However, there are no reports in the literature that establish the correlation between milk protein and casein precipitation quality, and then use the regression mathematical model to measure milk protein content and identify adulteration

Method used

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  • Milk protein detection method
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Experimental program
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Effect test

Embodiment 1

[0035] 1. Materials and methods

[0036] 1.1 Test material

[0037] Purchased 12 commercially available milk samples from the market in Alar City, Xinjiang, of which 9 samples were liquid pure milk processed by a dairy enterprise, 2 samples were commercially available fresh milk, and 1 sample was whole milk processed by a dairy enterprise milk powder.

[0038] Analytical reagents: glacial acetic acid, concentrated sulfuric acid, sodium hydroxide, boric acid, hydrochloric acid, copper sulfate, potassium sulfate, diammonium citrate, ammonium acetate, urea, ammonium persulfate, etc.

[0039] 1.2 Instruments and equipment

[0040] The equipment used in the test is as follows: TGL-16C centrifuge (Shanghai Anting Scientific Instrument Factory); JA5003N electronic balance: (Shanghai Qinghai Instrument Co., Ltd.); KDY-9830 Kjeldahl nitrogen analyzer: (Beijing Tongrunyuan Electromechanical Co., Ltd.) Technology Co., Ltd.); UL20AL rapid milk composition analyzer (Hangzhou Zheda Youch...

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Abstract

The invention belongs to the field of animal product detection and particularly relates to a milk protein detection method. A contract value (precipitation quality to milk volume) method is established to detect milk protein content, and the detection method for finding the milk protein content includes factors of milk standby time, centrifugation time, centrifugal rotational speed, ice acetic acid concentration and the like, and optimum parameters for precipitation of the milk protein are acquired, in other words, the standby time for the milk protein is 9 minutes, the centrifugation time is 7 minutes, the centrifugal rotational speed is 12000r / min, and the ice acetic acid concentration is 0.3mol / L; related data of the milk protein content and the contract value are subjected to partial lease squares analysis to acquire a regression linear equation of the milk protein content X to the contract value Y, namely the Y=0.0645X+0.0581, R2=9896, and higher linear correlation exists between the milk protein content X and the contract value Y within the scope ranging from 2.0g / 100mL to 4.0g / 100mL range.

Description

technical field [0001] The invention belongs to the technical field of animal product quality detection, and in particular relates to a method for detecting milk protein. Background technique [0002] Protein is one of the main components of milk and one of the most important quality indicators in milk testing. At present, there are many reports on milk protein detection methods at home and abroad, mainly Kjeldahl method, biuret method, Folin phenol method, Coomassie brilliant blue method, capillary electrophoresis method, ELISA method, high performance liquid chromatography, near-infrared method, etc. methods such as spectroscopy [1-9] . Among them, the Kjeldahl method is the most recommended protein detection method in the national and international standards, but the method is complicated to operate, takes a long time, and requires concentrated acid and alkali, which is dangerous to operate and produces harmful gases. In addition, the Kjeldahl method cannot distinguish...

Claims

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Application Information

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IPC IPC(8): G01N5/00
Inventor 蒲云峰孟庆艳穆晓建薛艳鹏吴建红张雅茹
Owner TARIM UNIV
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