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A graphene chip for capturing cancer cells in whole blood and its preparation method

A graphene and tumor cell technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of low efficiency and complicated methods for separation and counting of CTCs, and achieve high capture efficiency, high efficiency and high sensitivity, enrichment, enhancement The effect of the sticking effect

Active Publication Date: 2017-04-26
BEIJING NATURE STONE SCI TECH DEVP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current methods for isolating and counting CTCs are very complex and inefficient

Method used

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  • A graphene chip for capturing cancer cells in whole blood and its preparation method
  • A graphene chip for capturing cancer cells in whole blood and its preparation method
  • A graphene chip for capturing cancer cells in whole blood and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Add graphite powder into a flask containing fuming nitric acid and concentrated sulfuric acid, add potassium chlorate at zero degrees Celsius, and all processes are under the condition of nitrogen gas. The contents of the flask were then stirred at room temperature for more than 80 hours.

[0039] (2) Add hydrochloric acid to the product obtained in step (1), and wash until the sulfate group is eliminated.

[0040] (3) Wash the product obtained in step (2) with high-purity water until the pH value is neutral.

[0041] (4) The product obtained in step (3) is dried at high temperature to obtain powder.

[0042] (5) Weigh the powder obtained in step (4) to prepare a GO solution.

[0043] (6) The solution obtained in step (5) is vacuum filtered to form a film to form a graphene oxide film.

[0044] (7) Put the film obtained in step (6) into vacuum for heating and reduction, and the heating temperature is above 100 degrees Celsius.

[0045] (8) Put the reduced graphe...

Embodiment 2

[0057] (1) Add graphite powder into a flask containing fuming nitric acid and concentrated sulfuric acid, add potassium chlorate at zero degrees Celsius, and all processes are under the condition of nitrogen gas. The contents of the flask were then stirred at room temperature for more than 80 hours.

[0058] (2) Add hydrochloric acid to the product obtained in step (1), and wash until the sulfate group is eliminated.

[0059] (3) Wash the product obtained in step (2) with high-purity water until the pH value is neutral.

[0060] (4) The product obtained in step (3) is dried at high temperature to obtain powder.

[0061] (5) Weigh the powder obtained in step (4) to prepare a GO solution.

[0062] (6) The solution obtained in step (5) is vacuum filtered to form a film to form a graphene oxide film.

[0063] (7) Put the film obtained in step (6) into vacuum for heating and reduction, and the heating temperature is above 100 degrees Celsius.

[0064] (8) Put the reduced graphe...

Embodiment 3

[0076] (1) Add graphite powder into a flask containing fuming nitric acid and concentrated sulfuric acid, add potassium chlorate at zero degrees Celsius, and all processes are under the condition of nitrogen gas. The contents of the flask were then stirred at room temperature for more than 80 hours.

[0077] (2) Add hydrochloric acid to the product obtained in step (1), and wash until the sulfate group is eliminated.

[0078] (3) Wash the product obtained in step (2) with high-purity water until the pH value is neutral.

[0079] (4) The product obtained in step (3) is dried at high temperature to obtain powder.

[0080] (5) Weigh the powder obtained in step (4) to prepare a GO solution.

[0081] (6) The solution obtained in step (5) is vacuum filtered to form a film to form a graphene oxide film.

[0082] (7) Put the film obtained in step (6) into vacuum for heating and reduction, and the heating temperature is above 100 degrees Celsius.

[0083] (8) Put the reduced graphe...

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Abstract

The invention provides a graphene chip for capturing cancer cells in whole blood and a preparation method thereof, and relates to the field of biotechnology and clinic, in particular to a graphene biochip used for specifically identifying and capturing circulating cancer cells by using the surface with a multi-matching structure of a graphene material and a preparation method of the graphene biochip. A special structure is prepared on the surface of graphene and is entitled with a variety of chemical and physical properties matched with cells, then a specific identification antibody on the surface of a circulating cancer cell is fixed on the graphene surface with the special structure, the graphene is placed in a cell culture box, a sample (blood) to be measured is dropwise added onto the surface of the specific antibody on the surface of the cancer cell, and then the graphene is placed in a cell culture cabinet to achieve a multi-matching effect by means of the action of the specific antibody on the surface of the cancer cell and the surface of the special structure of the graphene, so that the circulating cancer cells in the sample to be measured can be effectively and accurately identified and captured. The method provided by the invention can be used for effectively capturing the circulating cancer cells, is low in cost, simple to operate and high in sensitivity, and can be applied to clinical detection.

Description

technical field [0001] The invention belongs to the technical field of functional materials, biomedical materials, in particular to a biochip for enrichment and detection of circulating tumor cells and a preparation method thereof. Background technique [0002] The detection, identification and isolation of circulating tumor cells (CTCs) are of great significance to the identification and understanding of cancer stem cell markers. Although the number of CTCs in blood is very small (several to hundreds per milliliter) mixed with a large number of blood cells (10 9 per milliliter), but it plays a very important role in detecting cancer metastasis, predicting a patient's diagnosis, and monitoring the outcome of treatment. For example, CTCs are isolated from primary tumors and then transported to different organs of the body through blood vessels. Epithelial cells derived from these living tumors are likely to be the source of cancer metastasis. This phenomenon has been demonst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/5302G01N33/57407
Inventor 王树涛李瑛颖江雷
Owner BEIJING NATURE STONE SCI TECH DEVP
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