Preparation and identification method of domestic silkworm silk fibroin solution

Abstract

The invention relates to the technical field of biomedicines, and in particular relates to a preparation and identification method of a domestic silkworm silk fibroin solution. The preparation method of the domestic silkworm silk fibroin solution sequentially comprises the following steps: (1) degumming, namely degumming domestic silkworm silks to remove silk sericin on the outer layer to obtain degummed silks; (2) dissolving the silks, namely dissolving the degummed silks by using a ternary solution containing calcium chloride to obtain a fibroin solution; (3) performing renaturation, namely sequentially performing dialysis treatment on the fibroin solution according to a concentration gradient from high to low by using a protein denaturant solution, and then performing water dialysis treatment to obtain a silk fibroin solution; and (4) storing, namely storing the silk fibroin solution. The invention also provides an identification method of the domestic silkworm silk fibroin solution. The method can be used for identifying the quality of the silk fibroin solution. The preparation method of the domestic silkworm silk fibroin solution disclosed by the invention is relatively high in economical performance, ensures that the molecular weight of the domestic silkworm silk fibroin solution is close to that of natural fibroin molecules, and is beneficial to the preparation of subsequent silk fibroin based biomaterials.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing and identifying silkworm silk fibroin solution. Background technique [0002] Silk is a product of ancient Chinese civilization. It has been widely used as clothing material since ancient times due to its softness, good air permeability and soft texture. In recent decades, because silk protein is non-toxic, non-irritating, has excellent biocompatibility, can promote the growth of human cells, and has certain biodegradability, it has been widely used in biomedicine, environmental protection and daily use. Technological fields such as chemical engineering are also developing continuously. [0003] Silk is mainly composed of two proteins, sericin and silk fibroin. Mulberry silk core-shell structure, the outer layer is coated with sericin (Sericin), and the inner layer is silk fibroin (Silk Fibroin), in which the silk fibroin content is 70-80%. Silk fibro...

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Problems solved by technology

In Chinese patent CN103194068A, urea is added to destroy silk fibroin to stabilize the silk fibroin solution, which weakens the effect of hydrogen bonds in the silk fibroin molecule or between several silk fibroin molecules, and improves the stability of silk fibroin. However, the introduction of urea is not conducive to silk fibroin. Peptide molecules are used in the research and application of drug delivery systems in vivo, or tissue engineering scaffolds

[0006] The shortcoming of silk degumming method in the prior art of table 1

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