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Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation

A technology of free anthraquinone and anthraquinone nanometers, applied in the field of pesticides, can solve the problems such as technical disclosure that the anti-powder mildew activity of nanoemulsion preparations is not found, and achieve the effects of easy mass production, simple preparation process and significant control effect.

Inactive Publication Date: 2015-05-27
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no technical disclosure of rhubarb free anthraquinone in the form of natural ratio of nanoemulsion and its anti-powdery mildew activity has been found

Method used

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  • Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation
  • Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation
  • Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the extraction of rhubarb free anthraquinone

[0018] Experimental conditions

[0019] Rhubarb was purchased from Lixian Xinshengyuan Biotechnology Development Co., Ltd. The crushed and sieved rhubarb powder was added to 20% sulfuric acid for acid hydrolysis, and the dregs were washed with water until neutral and then dried. Rhubarb hydrolyzed dry powder is mixed according to the raw material drug: 90% ethanol = 1:30 (w / v), ultrasonically extracted, suction filtered while hot, the filtrate is concentrated under reduced pressure until it has no alcohol smell, extracted with chloroform, and the chloroform extract is then 5% NaOH Extract until the supernatant is colorless, combine the alkaline extracts, adjust the pH to about 2, centrifuge to collect the precipitate, and wash with water until neutral to obtain the crude rhubarb free anthraquinone. After the crude product is dried, according to raw material: methanol = 1:10, add methanol for ultrasonic dissol...

Embodiment 2

[0020] Embodiment 2: Prescription and preparation of rhubarb free anthraquinone nanoemulsion

[0021] Prescription one:

[0022] Rhubarb free anthraquinone 0.130g, oil phase 25.5g, emulsifier 24.5g, co-emulsifier 12.3g, stabilizer 1.0g, preservative 0.1g, adhesive agent 0.1g, purified water 36.4g, total weight about 100.0g.

[0023] Prescription two:

[0024] Rhubarb free anthraquinone 0.048g, oil phase 22.2g, emulsifier 23.4g, co-emulsifier 11.7g, stabilizer 1.0g, preservative 0.1g, adhesive agent 0.1g, purified water 41.5g, total weight about 100.0g.

[0025] In the prescription: rhubarb free anthraquinone is the main drug, the oil phase is propylene glycol monocaprylate (prescription 2) or its mixture with ethyl oleate (prescription 1), the emulsifier is polyoxyethylene-40-hydrogenated castor oil, The auxiliary emulsifier is diethylene glycol monoethyl ether, the stabilizer is oleic acid, the preservative is sodium benzoate, and the adhesive agent is silicon trioxide.

...

Embodiment 3

[0028] Embodiment 3: Type, property, content determination and stability of rhubarb free anthraquinone nanoemulsion

[0029] Test condition: to the nanoemulsion that embodiment 2 prescription one obtains, carry out following test:

[0030] Identification of nanoemulsion type: the type of nanoemulsion is identified by dyeing method, and the type of nanoemulsion is judged by the diffusion speed of oil-soluble dye Sudan red and water-soluble dye methylene blue in nanoemulsion.

[0031] Nanoemulsion properties: appearance, light transmittance, pH value, viscosity, particle size and Zeta potential.

[0032] Content determination: take 1 ml of rhubarb free anthraquinone nanoemulsion, add 10 ml of methanol, ultrasonically break the emulsion, filter through a 0.45 μm microporous membrane, analyze with high performance liquid chromatography (HPLC), and calculate the free anthracene in rhubarb free anthraquinone nanoemulsion quinone content.

[0033]Stability investigation: (1) Influe...

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Abstract

The invention belongs to the technical field of biopesticides, and particularly relates to a natural-proportion composition of rheum officinale free anthraquinone as well as a formula of a nano emulsion preparation of the composition, a preparation process of the composition and application of the composition for preventing powdery mildew. Monomer emodin, aloe-emodin, chrysophanol, emodin monomethyl ether and rhein in the rheum officinale free anthraquinone composition are extracted from the traditional Chinese medicine rheum officinale and provided with a natural proportion with a fixed range. The rheum officinale free anthraquinone composition and the nano emulsion preparation thereof can be used for preventing the plant powdery mildew. The free anthraquinone in the rheum officinale free anthraquinone nano emulsion disclosed by the invention is from the traditional Chinese medicine rheum officinale, the extracting process is strong in applicability, the raw material obtaining cost is low, the nano emulsion preparation is spontaneously formed, the process is simple, the quality is controllable, the large-scale production is easy, and an excellent prevention effect is shown for the plant powdery mildew.

Description

technical field [0001] The invention belongs to the field of pesticides, in particular to a rhubarb free anthraquinone composition and a nanoemulsion preparation for preventing and treating plant powdery mildew. Background technique [0002] Plant powdery mildew widely exists on the stems and leaves of various crops, vegetables, flowers, trees, etc., and is mainly caused by plant powdery mildew. Powdery mildew has a short incubation period, can reproduce multiple generations in one growing season, and has a high reproduction rate. The spores produced can spread in a wide range by means of the air, and repeatedly infect host plants. After the host is infected by powdery mildew, the respiration is enhanced, the transpiration intensity is increased, and the photosynthetic efficiency is reduced, which seriously hinders the normal growth and development of plants and causes huge economic losses. At present, chemical fungicides such as triadimefon, benzimidazoles, organophospho...

Claims

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Application Information

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IPC IPC(8): A01N37/42A01N35/06A01N25/04A01P3/00
Inventor 史彦斌李会丽李红玉郑果李金成
Owner LANZHOU UNIVERSITY
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