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Bacillus thuringiensis dispersible oil suspoemulsion and preparation method thereof

A technology of Bacillus thuringiensis and Bacillus aureus, applied in botany equipment and methods, insecticides, biocides, etc., can solve problems such as poor pourability, inconsistent impact effects, and large influence of additives on spores and paraspore crystals, etc. Achieve the effect of good dispersion, low production cost, and no sedimentation

Active Publication Date: 2015-05-27
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing problem is that commonly used adjuvants have a greater impact on spores and paraspore crystals, and the effects of the same adjuvant on paraspore crystals and spores may also be inconsistent.
Occurrence of Ostwald ripening will lead to creaming of dispersible oil suspoemulsion, making pourability poor

Method used

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  • Bacillus thuringiensis dispersible oil suspoemulsion and preparation method thereof
  • Bacillus thuringiensis dispersible oil suspoemulsion and preparation method thereof
  • Bacillus thuringiensis dispersible oil suspoemulsion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of the bacillus thuringiensis raw powder is as follows: after the bacillus thuringiensis is fermented and centrifuged and concentrated, the concentrated product is freeze-dried or spray-dried to obtain a spore crystal mixture. The original powder of Bacillus thuringiensis is passed through a 200-mesh sieve.

[0018] Weigh 4g of medium-chain triglyceride (dissolve 2mg of antioxidant tert-butylhydroquinone in 4g of medium-chain triglyceride oil), 804.8g of Tween, 1.2g of absolute ethanol and stir at room temperature to obtain an oil dispersion; Take by weighing 3.3g of the former powder of Bacillus thuringiensis and add the above-mentioned oil dispersant, mix evenly with 600r / min for 10min, the rotating speed of the sand mill shaft is 2600r / min, and the grinding time is 1h, so that the particle diameter of the former powder of Bacillus thuringiensis in the emulsion is 2 μm to obtain Bacillus thuringiensis dispersible oil suspoemulsion.

Embodiment 2

[0020] The preparation method of the former powder of Bacillus thuringiensis is the same as that in Example 1.

[0021] Weigh 4 g of medium-chain triglycerides (dissolve 0.6 mg of antioxidant tert-butyl hydroquinone in 4 g of medium-chain triglyceride oil), 804.8 g of Tween, and 1.2 g of absolute ethanol and stir at room temperature to obtain an oil dispersion Take by weighing 4.5g of the former powder of Bacillus thuringiensis and add the above-mentioned oil dispersant, mix evenly with 700r / min for 10min, the rotating speed of the sand mill shaft is 2600r / min, and the grinding time is 1h, so that the particle diameter of the former powder of Bacillus thuringiensis in the emulsion 2 μm, that is, Bacillus thuringiensis dispersible oil suspoemulsion.

Embodiment 3

[0023] The preparation method of the former powder of Bacillus thuringiensis is the same as that in Example 1.

[0024] Weigh 5.0 g of medium-chain triglyceride (dissolve 2.0 mg of antioxidant tert-butyl hydroquinone in 5.0 g of medium-chain triglyceride oil), 803.0 g of Tween, and 2.0 g of absolute ethanol at room temperature and stir evenly to prepare Oil dispersant: take 2.0g of Bacillus thuringiensis former powder and add the above-mentioned oil dispersant, mix evenly with 600r / min for 8min, the rotational speed of the sand mill shaft is 2600r / min, and the grinding time is 1h, so that the former powder of Bacillus thuringiensis in the emulsion The particle size is 2 μm, and the dispersible oil suspoemulsion of Bacillus thuringiensis is obtained.

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Abstract

The invention discloses a bacillus thuringiensis dispersible oil suspoemulsion and a preparation method thereof. The preparation method comprises the following steps: uniformly mixing 0.000-0.002 part of tert-Butylhydroquinone, 1-2.5 parts of absolute ethyl alcohol, 2-5 parts of tween 80 and 3-5 parts of median chain triglyceride oil to prepare an oil dispersing agent, and then adding 2-5 parts of bacillus thuringiensis into the oil dispersing agent for uniformly stirring and stirring to prepare the bacillus thuringiensis dispersible oil suspoemulsion. The suspoemulsion cannot kill or suppress germs and has the advantages of high flowability, no sediment layering, stable preparation, high water dispersibility and no harm to bacillus thuringiensis and parasporal crystal proteins.

Description

technical field [0001] The invention relates to an insecticide, in particular to a bacillus thuringiensis dispersible oil suspoemulsion and a preparation method thereof. Background technique [0002] Bacillus thuringiensis is a Gram-positive bacillus that can be isolated from insects, soil, stored products, dust, sewage and plants. Its preparation is currently the microbial insecticide with the largest output and better insecticidal effect in the world. Bacillus thuringiensis has the advantages of being highly effective against pests, safe for humans and animals, and environmentally friendly, and can be widely used in the prevention and control of agricultural, forestry, fruit, vegetable, and urban garden plant pests, stored pests, and sanitary pests. [0003] The commonly used formulations of Bacillus thuringiensis preparations are mainly aqueous suspensions with water as the medium and insecticidal powder with solid fillers as the medium. Bacillus thuringiensis aqueous s...

Claims

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Application Information

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IPC IPC(8): A01N25/04A01N63/00A01P7/04
Inventor 王春维李奥祝爱侠冯伟彭凯迪
Owner WUHAN POLYTECHNIC UNIVERSITY
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