Solid biological reaction device and method utilizing same to prepare filamentous bacterium spores

Abstract

Provided in the present invention is a solid-state biological reaction device, comprising a main tank body and a collector, wherein a top air outlet, a bottom air inlet and a material entrance and exit are provided on the main tank body, wherein the collector is connected to the main tank body through the top air outlet. Also provided in the present invention is a method for preparing filamentous fungus spores utilizing the solid-state biological reaction device, comprising the steps of: feeding a culture substrate into the main tank body through the material entrance and exit, performing steam sterilization by injecting steam through the bottom air inlet, inoculating the filamentous fungus strains onto a sterile culture substrate in the main tank body for culturing so as to obtain mature filamentous fungus spores, and then passing dry air into the main tank body through the bottom air inlet, so that the filamentous fungus spores enter into the collector through the top air outlet. The solid-state biological reaction device of the present invention can achieve the mechanization and large-scale preparation of filamentous fungus spores.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a solid-state biological reaction device and a method for preparing filamentous fungal spores by using the solid-state biological reaction device. Background technique [0002] In the field of industrial fermentation, the expansion and cultivation of strains is an important part of the fermentation process. Except for a very small number of microorganisms such as alcoholic yeast and lactic acid bacteria that have professional strain production factories that produce commercial strains that are directly used in fermentation production, in fermentation enterprises, the process of expanding the strains from slant strains to first-level seed tanks has always been It is the process with the lowest degree of mechanization and takes up a lot of manual labor. [0003] Filamentous fungi have a filamentous structure (hyphae), such as filamentous fungi and actinomycetes, and filam...

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Problems solved by technology

[0007] Fermentation production uses the gluten spores cultivated in a large number of triangular flasks. In addition to the above-mentioned problems, there are also following problems that are difficult to overcome: first, the preparation process of triangular flasks is complicated, and it is difficult to check the quality of gluten spores bottle by bottle. , it is very easy to contaminate miscellaneous bacteria and cause the fermentation tank to be contaminated; secondly, each fermentation tank is connected with dozens of bottles or even hundreds of bottles of gluten spores, which requires a lot of labor and many chances of infection during the inoculation process; thirdly, the inoculation of spores At the same time, the bran in the bran koji is also connected to the fermentation tank, which increases the impurities in the fermentation liquid, and is not suitable for fermentation with high requirements for the impurity content of the fermentation liquid; in addition, to prepare a large amount of bran koji spores, a large-scale The constant temperature curved room, the construction investment is relatively large

The structure and configuration of the spore box are complicated; the cleaning and preparation time is long; the manual labor is still relatively large; the aseptic requirements for equipment, environment and operation are extremely high. During the operation of the process, if there is a slight carelessness, it will cause contamination by bacteria; after chemical fumigation and sterilization, not only must the sterilant be completely removed to avoid the impact of the residue of the chemical sterilant on the cultured microorganisms, but also must be adsorbed or neutralized. and sterilants to prevent environmental pollution by chemical sterilants; agar medium is used for shallow plate solid culture, and the cost of the medium is high; negative pressure is used to collect spores, which has extremely high requirements for equipment to prevent contamination by bacteria; some spores cannot be collected into the spore collection bottle

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