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SERS (surface-enhanced Raman spectrum) detection method for narcotics in urine sample

A detection method and urine sample technology, applied in the field of purified SERS detection and rapid separation, can solve the problems of high detection technology requirements, long detection time, complicated operation, etc., and achieve the goal of improving sensitivity, accuracy, and detection efficiency Effect

Active Publication Date: 2015-05-13
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, common detection methods for drugs include chromogenic method, chromatography, molecular spectroscopy, capillary electrophoresis, colloidal gold method, etc., but each of these methods has low sensitivity, expensive reagents for detection, high detection technology requirements, and long detection time. Long, complex operation, false positives, etc.

Method used

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  • SERS (surface-enhanced Raman spectrum) detection method for narcotics in urine sample
  • SERS (surface-enhanced Raman spectrum) detection method for narcotics in urine sample
  • SERS (surface-enhanced Raman spectrum) detection method for narcotics in urine sample

Examples

Experimental program
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Effect test

Embodiment 1

[0040] (1) First transfer gold nanoparticles with a diameter of 5nm to cyclohexane to form a sol; then add the sol to sodium dodecyl sulfate, stir to form an oil-in-water emulsion, remove cyclohexane and After sodium dodecyl sulfate, a three-dimensional configuration of sol supercrystalline nanomaterials is formed;

[0041] (2) Soak the coverslip in aqua regia, concentrated sulfuric acid and hydrogen peroxide in sequence, then soak and wash it in ethanol, and dry it with nitrogen to get a clean and flat substrate;

[0042] (3) Drop the sol supercrystalline nanomaterial in (1) onto the substrate treated in (2), and dry to obtain the SERS substrate;

[0043] (4) Take 2 mL of urine sample into a 4 mL EP tube, and add 0.1 mL of reagent 1 sodium hydroxide and 0.5 g of reagent 2 solid sodium chloride to the urine sample in turn, mix well and then add 0.5 g of reagent tricyclohexane mL, and then extract it by shaking;

[0044] (5) After centrifuging for 2 minutes, take the organic ...

Embodiment 2

[0047] (1) First transfer silver nanosheets with a diameter of 10 nm to ethyl acetate to form a sol; then add the sol to hexadecyltrimethylammonium bromide, stir to form an oil-in-water emulsion, and transfer After removing ethyl acetate and cetyltrimethylammonium bromide, a three-dimensional configuration of sol supercrystalline nanomaterials is formed;

[0048] (2) After soaking the quartz sheet in aqua regia, concentrated sulfuric acid and hydrogen peroxide in sequence, soak it in ethanol and wash it, and dry it with nitrogen to get a clean and flat substrate;

[0049] (3) Drop the sol supercrystalline nanomaterials onto the substrate and dry to obtain the SERS substrate;

[0050] (4) Take 2 mL of urine sample into a 4 mL EP tube, add 0.2 mL of reagent one potassium hydroxide and 1.0 g of reagent two solid sodium sulfate to the urine sample in turn, mix well and then add 0.2 mL of reagent trimethylbenzene, and then its vibration extraction;

[0051] (5) After filtering wi...

Embodiment 3

[0055] (1) First transfer copper-gold nanorods with a diameter of 20 nm into ether to form a sol; then add the sol to polyvinylpyrrolidone, stir to form an oil-in-water emulsion, remove ether and polyethylene After pyrrolidone, a three-dimensional configuration of sol supercrystalline nanomaterials is formed;

[0056] (2) Soak silicon wafers in aqua regia, concentrated sulfuric acid and hydrogen peroxide sequentially, then soak and wash them in ethanol, and dry them with nitrogen to obtain a clean and flat substrate;

[0057] (3) Drop the sol supercrystalline nanomaterials onto the substrate and dry to obtain the SERS substrate;

[0058] (4) Take 2 mL of urine sample in a 4 mL EP tube, add 0.3 mL of reagent 1 ammonia water and 0.8 g of reagent 2 solid ammonium carbonate to the urine sample in turn, mix well, then add 0.5 mL of reagent triethyl ether, and then vibration extraction;

[0059] (5) After centrifuging for 2 minutes, take the organic layer solution;

[0060] (6) A...

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Abstract

The invention discloses an SERS (surface-enhanced Raman spectrum) detection method for narcotics in a urine sample. The method comprises steps as follows: synthesizing a sol ultrafine grained nano-material with SERS active noble metals; dropping the sol ultrafine grained nano-material onto a substrate, and obtaining an SERS base through drying; taking the urine sample, sequentially adding a reagent I, namely, an alkaline solution, and a reagent II, namely, solid salt, to the urine sample, adding a reagent III, namely, an organic solvent, after even mixing, and then performing vibrating extraction on the mixture; taking an organic-layer solution after centrifugation or filtration by the aid of an organic filtration membrane; dropping the organic-layer solution onto the SERS base, and performing SERS detection on the organic-layer solution by the aid of a Raman spectrometer. According to the method, the urine sample of a person is pretreated, and the common narcotics in the urine sample are quickly separated, purified and enriched, so that the detection time is shortened, only 3-5 min is taken for the whole detection, and the detection sensitivity and the detection efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical field of chemical component detection, in particular to a SERS detection method for rapid separation and purification of common drugs in human urine samples. Background technique [0002] The addiction and prevalence of drug abuse has become an increasingly serious problem in today's world, such as methamphetamine (MAMP, commonly known as "ice"), 3,4-methylenedioxymethamphetamine (MDMA, commonly known as "ecstasy") and other amphetamine-type drugs (ATS). Because of its easy-to-obtain raw materials, simple production process, excitatory central nervous system, suppress appetite, cause euphoria, hallucinations and other effects in users, repeated use is easy to make people addicted, etc. It has become a popular drug among drug addicts and drug dealers in the world today. One of the most popular drugs in The prevalence of drugs not only destroys families, causes social unrest, but also leads to the spread of dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/40G01N1/34
Inventor 刘洪林韩珍珍杨良保刘锦淮
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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