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Method for rapidly detecting biogenic amine by thin layer chromatography in one step

A technology of thin-layer chromatography and biogenic amines, applied in measuring devices, analysis materials, instruments, etc., can solve the problems of long preheating equilibrium and analysis time, immature technology, and few complete sets of equipment, etc., to shorten the detection time , detection time is shortened, the effect of easy operation

Active Publication Date: 2015-05-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all have certain applications, and each has its own advantages and disadvantages; for example, the most commonly used RP-HPLC method is generally believed to have the advantages of high detection sensitivity, accurate quantitative analysis, and high column efficiency, and there are many standard methods based on this technology. ; but this method requires high performance liquid chromatography, and the process of instrument preheating, equilibration and analysis takes a long time, the speed is slow, and the flexibility is poor
Biosensors are highly regarded due to their online monitoring capabilities, but the existing technology is still not very mature, there are few reliable complete sets of equipment in the market, and the price is relatively expensive

Method used

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  • Method for rapidly detecting biogenic amine by thin layer chromatography in one step
  • Method for rapidly detecting biogenic amine by thin layer chromatography in one step
  • Method for rapidly detecting biogenic amine by thin layer chromatography in one step

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the preparation of activated silica gel

[0048] Accurately weigh 10g of 200-300 mesh silica gel imported from Merck, Germany, first make a suspension with 50ml DMSO, then add 20ml of epichlorohydrin, shake well; accurately weigh 1.6g of sodium hydroxide, dissolve it in 100ml of deionized water Prepare a 0.4mol / L NaOH solution; finally add 100ml 0.4mol / L NaOH solution to the above-mentioned silica gel activation solution; put the silica gel activation system into a constant temperature shaker at 30°C with a rotation speed of 180rpm for 8 hours, after the activation is completed, filter Remove the liquid system and wash the medium with copious amounts of deionized water until the pH of the filtrate is less than 8.

[0049] Take 30mL of ammonia water with a concentration of 25% and 120mL of deionized water, mix and prepare 5% ammonia water as the activation solution; accurately weigh 10g of epoxy activated silica gel and place it in a 250ml Erlenmeyer flask,...

Embodiment 2

[0051] Embodiment 2: the preparation of activated silica gel

[0052] Accurately weigh 10g of 100-200 mesh domestic silica gel, first make a suspension with 125ml DMSO, then add 60ml of phenoxypropylene oxide, shake well; accurately weigh 3.2g of sodium hydroxide, dissolve it in 100ml of deionized water 0.8mol / L NaOH solution; finally, add 100ml 0.8mol / L NaoH solution to the above silica gel activation solution; put this silica gel activation system into a constant temperature shaker at 35°C and a speed of 180rpm to react for 11 hours, after the activation is completed, filter out the liquid system, And wash the medium with a large amount of deionized water until the pH of the filtrate is less than 8.

[0053] Take 90mL of chloramine with a concentration of 25% and 135mL of deionized water, mix and prepare 10% chloramine as an activation solution system; accurately weigh 10g of epoxy activated silica gel and place it in a 500ml Erlenmeyer flask, add 225mL of 10% chloramine, I...

Embodiment 3

[0055] Embodiment 3: the preparation of activated silica gel

[0056] Accurately weigh 10g of 200-300mesh silica gel imported from Merck, Germany, first make a suspension with 200ml DMSO, then add 100ml of epichlorohydrin, shake well; accurately weigh 4.8g of sodium hydroxide, dissolve in 100ml of deionized water Prepare 1.2mol / L NaOH solution; finally add 100ml 1.2mol / L NaOH solution to the above-mentioned silica gel activation solution; put this silica gel activation system in a constant temperature shaker at 40°C with a rotation speed of 180rpm for 14h, after the activation is completed, filter Remove the liquid system and wash the medium with copious amounts of deionized water until the pH of the filtrate is less than 8.

[0057] Take 180mL of ethylenediamine with a concentration of 25% and 120mL of deionized water, mix and prepare 15% ethylenediamine as an activation solution system; accurately weigh 10g of epoxy-activated silica gel and place it in a 500ml Erlenmeyer fla...

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Abstract

The invention discloses a method for rapidly detecting biogenic amine by thin layer chromatography in one step and belongs to the technical field of food analysis and safety. The method disclosed by the invention comprises the following steps: (1) preparing an activated silica gel; (2) preparing an activated thin layer chromatography plate; and (3) analyzing the thin layer chromatography in the biogenic amine in a food sample. The method for rapidly detecting biogenic amine by thin layer chromatography in one step, disclosed by the invention, can be used for rapidly measuring the content of biogenic amine in products such as aquatic products, meat, diary products, pickled vegetables, spirits and esters. Compared with other analytical methods, according to the method, a derivating agent is directly fixed on a medium during activation, and the extracted and purified sample needs not to be derived, is diluted to a calibrated range and can be directly dispensed to be expanded for measurement; moreover, no expensive analytical instruments are required, so that the method is low in expense and simple and rapid to operate, and can effectively lower the monitoring cost in food production and circulation.

Description

technical field [0001] The invention relates to a method for rapidly detecting biogenic amine by one-step thin-layer chromatography, which belongs to the technical field of food analysis and safety. Background technique [0002] Biogenic amine is a general term for a class of biologically active nitrogen-containing low-molecular-weight organic compounds; foods containing protein and amino acids can produce biogenic amines through decarboxylation of amino acids or amination of aldehydes under certain conditions. It has been found that aquatic products, meat products, cheese and dairy products, wine and various fermented foods, vegetables, fruits, dried fruits and chocolates contain a variety of biogenic amines. Excessive biogenic amines have been proven to cause some adverse physiological reactions to the human body. In food preservation, biogenic amines are often produced during food decay or fermentation; histamine and tyramine are the most abundant in foods and are also ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 夏小乐张无疾张清文马吉王武周哲敏
Owner JIANGNAN UNIV
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