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Method for extracting quercetin from eleocharis tuberosa peels

A technique for water chestnut bark and quercetin, which is applied in the field of extraction and separation of quercetin, and the extraction and separation of quercetin from water chestnut bark, can solve unseen problems, and achieves low production cost, good separation effect, and easy availability of raw materials. Effect

Active Publication Date: 2015-05-06
HEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In our research, we found that water chestnut skin is rich in quercetin, but there is no literature report on the extraction and isolation of this compound from water chestnut skin

Method used

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  • Method for extracting quercetin from eleocharis tuberosa peels

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Experimental program
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Effect test

Embodiment 1

[0025] Add 8kg of fresh water chestnut skin powder into the extraction tank, add 32L of 70% by volume acetone aqueous solution, soak at 25°C for 24 hours, soak for 3 times in total, filter, combine the filtrates, concentrate under reduced pressure to obtain 1.05kg of extract .

[0026] The extract was added to 5.0 L of water to make a turbid liquid, extracted three times with 5.0 L of ethyl acetate, the extracts were combined and concentrated under reduced pressure to obtain 120 g of the extract.

[0027] Add 300mL methanol to the extract to dissolve, mix the sample with 260g polyamide, volatilize the methanol to dryness, install the column, connect to the MCI column for medium pressure separation, use 40-100% by volume methanol aqueous solution as the mobile phase gradient elution, Collected in 500 mL portions, detected by thin-layer chromatography, collected the eluent with a combined mobile phase concentration of 65-69%, concentrated under reduced pressure to obtain 9.8 g o...

Embodiment 2

[0032] Add 8kg of fresh water chestnut skin powder into the extraction tank, add 32L of 70% by volume acetone aqueous solution, soak at 25°C for 24 hours, soak for 3 times in total, filter, combine the filtrates, concentrate under reduced pressure to obtain 1.05kg of extract .

[0033] The extract was added to 5.0 L of water to make a turbid liquid, extracted three times with 5.0 L of ethyl acetate, the extracts were combined and concentrated under reduced pressure to obtain 120 g of the extract.

[0034] Add 300mL methanol to the extract to dissolve, mix the sample with 260g polyamide, volatilize the methanol to dryness, install the column, connect to the MCI column for medium pressure separation, use 40-100% by volume methanol aqueous solution as the mobile phase gradient elution, Collected in 500 mL portions, detected by thin-layer chromatography, collected the eluent with a combined mobile phase concentration of 65-69%, concentrated under reduced pressure to obtain 9.8 g o...

Embodiment 3

[0039] Add 8kg of fresh water chestnut skin powder into the extraction tank, add 32L of 70% by volume acetone aqueous solution, soak at 25°C for 24 hours, soak for 3 times in total, filter, combine the filtrates, concentrate under reduced pressure to obtain 1.05kg of extract .

[0040] The extract was added to 5.0 L of water to make a cloudy liquid, extracted three times with 5.0 L of ethyl acetate, the combined extracts were concentrated under reduced pressure to obtain 120 g of the extract.

[0041] Add 300mL methanol to the extract to dissolve, mix the sample with 260g polyamide, volatilize the methanol to dryness, install the column, connect to the MCI column for medium pressure separation, use 40-100% volume percent methanol aqueous solution as the mobile phase gradient elution, Collected in 500 mL portions, detected by thin-layer chromatography, collected the eluent with a combined mobile phase concentration of 65-69%, concentrated under reduced pressure to obtain 9.8 g ...

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Abstract

The invention discloses a method for extracting quercetin from eleocharis tuberosa peels and belongs to the field of natural organic chemistry. The method is characterized by comprising the following steps of by using an acetone aqueous solution as a solvent, extracting quercetin from the eleocharis tuberosa peels, extracting an extract with ethyl acetate, separating an extractant with a middle-pressure MCI column chromatography and a polyamide column chromatography and purifying with Sephadex LH-20 gel and silica gel column chromatography to obtain a quercetin product of which the content of quercetin is not less than 95%. The method opens up a novel way for extracting and separating quercetin from natural plants, the good separation effect is achieved, the product is high in purify, the eleocharis tuberosa peels, which are always regarded as wastes, are changed into treasure, the selected chromatographic material MCI, polyamide and gel can be repeatedly used, the raw materials are easily available and are rich in resources, the method is low in production cost, the large-scale production can be performed and the method meets the needs of pharmaceutical industry.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry and relates to a method for extracting and separating quercetin, in particular to a method for extracting and separating quercetin from water chestnut skin. Background technique [0002] Quercetin, whose chemical name is 3,5,7,3′,4′-pentahydroxyflavone, also known as quercetin and quercetin, is the most widely distributed flavonol compound in the plant kingdom. In 68% of plants, it often exists in the flowers, leaves and fruits of plants. Chinese herbal medicines such as Huai Mi, red star anise, red eclipta, kenaf leaves, and ginkgo contain a lot of quercetin; foods such as onions, grapes, apples, red wine, and green tea are also rich in this ingredient. Quercetin has good biological activities, such as anti-tumor, anti-oxidation, anti-inflammation, anti-virus, anti-bacterial and other activities and expectorant, cough, anti-platelet aggregation, lowering blood pressure, lowering blood fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 刘珊罗杨合李行任商飞飞杨杰龙
Owner HEZHOU UNIV
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