Method for preparing fluorescent carbon quantum dot
A technology for carbon quantum dots and fluorescence, which is applied in the field of preparing fluorescent carbon quantum dots, can solve the problems of low yield, long cycle and high cost, and achieves the effects of high fluorescence performance and improved fluorescence efficiency.
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[0064] The present invention also provides a preparation method of the immunochromatographic test strip, comprising:
[0065] Step 1: Spray the antibody combined with fluorescent carbon quantum dots on the conjugate release pad to obtain the conjugate release area;
[0066] Step 2: Spray the antigen and anti-mouse IgG antibody on the solid support in the reaction area to form a test area and a control area respectively to obtain a reaction area;
[0067] Step 3: Paste the sample loading area, the conjugate release area, the reaction area and the water absorption area on the backing in order to overlap each other.
[0068] Those skilled in the art can understand that the antibodies and antigens sprayed on the immunochromatographic test strip of the present invention are directed against different detection substances and select antibodies and antigens suitable for the detection substances.
[0069] The present invention also provides an immunochromatography kit, including the ...
Embodiment 1
[0084] Embodiment 1, detection method and calculation method of fluorescence quantum efficiency
[0085] The fluorescence quantum efficiency was determined by the reference method. By selecting the wavelength corresponding to the intersection point of the absorption curve of the standard solution and the analyte solution in the absorption spectrum as the excitation wavelength, the analyte and the standard are excited to obtain the corresponding fluorescence intensity. The fluorescent quantum efficiency of quinine sulfate in 0.05 mol / L sulfuric acid solution was selected as 55% as a standard, and the fluorescent quantum efficiency of fluorescent carbon quantum dots was calculated. Fluorescence quantum efficiency of carbon quantum dots The calculation formula is:
[0086]
[0087] Where (c) and (q) represent fluorescent carbon quantum dots and quinine sulfate, respectively; A represents the absorption value at the excitation wavelength, I represents the fluorescence peak a...
Embodiment 2
[0088] Embodiment 2, the preparation of fluorescent carbon quantum dot
[0089] Take 1g of carrageenan and add 20g of water to mix, place in a closed system, heat to 220-240°C, react for 4h, stop heating, cool to room temperature, centrifuge the reaction solution at 10000rpm for 30min, take the supernatant to obtain fluorescence carbon quantum dots. The particle size of the prepared fluorescent carbon quantum dots detected by the electron microscope is about 2nm. The fluorescent quantum efficiency of the fluorescent carbon quantum dots prepared according to the method described in Example 1 is 35.5%. The absorbance and fluorescence intensity of the prepared fluorescent carbon quantum dots with different concentrations at different wavelengths and the fluorescence intensity of fluorescent carbon quantum dots in aqueous solution under different concentration gradients were detected. The results are shown in Figure 2-4 .
[0090] Depend on Figure 2-4 The results show that t...
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