Method for preparing fluorescent carbon quantum dot

A technology for carbon quantum dots and fluorescence, which is applied in the field of preparing fluorescent carbon quantum dots, can solve the problems of low yield, long cycle and high cost, and achieves the effects of high fluorescence performance and improved fluorescence efficiency.

Inactive Publication Date: 2015-04-29
SINOCARE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the methods for preparing carbon quantum dots with fluorescent properties include: high temperature and high pressure excision method; candle burning method; electrochemical scanning method; chemical oxidation method, etc. These methods often have some limitations in making carbon quantum dots, such as: high cost and high yield Low, dialysis is used in the purification process, the cycle is long, etc.

Method used

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  • Method for preparing fluorescent carbon quantum dot
  • Method for preparing fluorescent carbon quantum dot
  • Method for preparing fluorescent carbon quantum dot

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preparation example Construction

[0064] The present invention also provides a preparation method of the immunochromatographic test strip, comprising:

[0065] Step 1: Spray the antibody combined with fluorescent carbon quantum dots on the conjugate release pad to obtain the conjugate release area;

[0066] Step 2: Spray the antigen and anti-mouse IgG antibody on the solid support in the reaction area to form a test area and a control area respectively to obtain a reaction area;

[0067] Step 3: Paste the sample loading area, the conjugate release area, the reaction area and the water absorption area on the backing in order to overlap each other.

[0068] Those skilled in the art can understand that the antibodies and antigens sprayed on the immunochromatographic test strip of the present invention are directed against different detection substances and select antibodies and antigens suitable for the detection substances.

[0069] The present invention also provides an immunochromatography kit, including the ...

Embodiment 1

[0084] Embodiment 1, detection method and calculation method of fluorescence quantum efficiency

[0085] The fluorescence quantum efficiency was determined by the reference method. By selecting the wavelength corresponding to the intersection point of the absorption curve of the standard solution and the analyte solution in the absorption spectrum as the excitation wavelength, the analyte and the standard are excited to obtain the corresponding fluorescence intensity. The fluorescent quantum efficiency of quinine sulfate in 0.05 mol / L sulfuric acid solution was selected as 55% as a standard, and the fluorescent quantum efficiency of fluorescent carbon quantum dots was calculated. Fluorescence quantum efficiency of carbon quantum dots The calculation formula is:

[0086]

[0087] Where (c) and (q) represent fluorescent carbon quantum dots and quinine sulfate, respectively; A represents the absorption value at the excitation wavelength, I represents the fluorescence peak a...

Embodiment 2

[0088] Embodiment 2, the preparation of fluorescent carbon quantum dot

[0089] Take 1g of carrageenan and add 20g of water to mix, place in a closed system, heat to 220-240°C, react for 4h, stop heating, cool to room temperature, centrifuge the reaction solution at 10000rpm for 30min, take the supernatant to obtain fluorescence carbon quantum dots. The particle size of the prepared fluorescent carbon quantum dots detected by the electron microscope is about 2nm. The fluorescent quantum efficiency of the fluorescent carbon quantum dots prepared according to the method described in Example 1 is 35.5%. The absorbance and fluorescence intensity of the prepared fluorescent carbon quantum dots with different concentrations at different wavelengths and the fluorescence intensity of fluorescent carbon quantum dots in aqueous solution under different concentration gradients were detected. The results are shown in Figure 2-4 .

[0090] Depend on Figure 2-4 The results show that t...

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Abstract

The invention belongs to the technical field of the immunochromatographic and in particular relates to a method for preparing a fluorescent carbon quantum dot, the prepared fluorescent carbon quantum dot and application of the prepared fluorescent carbon quantum dot to an immunochromatographic strip. According to the method for preparing the fluorescent carbon quantum dot, animal and plant colloids are used as raw materials to prepare the carbon quantum dot, the animal and plant colloid contains conjugated molecules such as benzene ring and can form a conjugated agglomerated carbon quantum dot in a reaction process, and the fluorescent carbon quantum dot has high fluorescence property; and furthermore, a polymer is added in a heating reaction process to effectively improve the fluorescence efficiency of the fluorescent carbon quantum dot; and experimental results show that the fluorescence quantum efficiency of the fluorescent carbon quantum prepared by the method can reach 30-50%.

Description

technical field [0001] The invention belongs to the technical field of immunochromatography, in particular to a method for preparing fluorescent carbon quantum dots, in particular to a method for preparing fluorescent carbon quantum dots, the prepared fluorescent carbon quantum dots and their application on immunochromatographic test strips . Background technique [0002] Immunochromatography is a unique immunoassay method that appeared in the early 1980s. It often uses strip-shaped fiber chromatography materials as the solid phase, and the sample solution swims on the chromatography strip through capillary action, and at the same time makes the sample solution The analyte and the receptor (such as antibody or antigen) for the analyte on the chromatographic material have a highly specific and high-affinity immune reaction, and the immune complex is enriched or trapped in the chromatographic material during the chromatography In a certain area (detection zone), an intuitive ...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N27/26
CPCG01N33/533
Inventor 吴天景
Owner SINOCARE
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