Clostridium butyricum and application thereof

A technology of Clostridium butyricum and Clostridium, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of environmental pollution and high cost of raw materials, and achieve the effect of high tolerance, low cost and renewable raw materials

Inactive Publication Date: 2015-04-29
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical route is to synthesize 1,3-propanediol from chemical raw materials through a series of chemical catalytic reactions. The cost of raw materials in this method is high, and the reagents used are likely to cause environmental pollution.

Method used

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  • Clostridium butyricum and application thereof
  • Clostridium butyricum and application thereof
  • Clostridium butyricum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Isolation and purification of strains

[0033] (1) Take 1 g of the collected sample, add it to 9 mL of Clostridium proliferation liquid medium, and culture for 24 hours at 37°C in a sealed enrichment;

[0034] (2) Transfer to seed culture medium, perform anaerobic selective enrichment culture at 37°C for 24 hours, and then perform high-performance liquid chromatography to analyze the fermentation product, and select positive bacteria capable of producing 1,3-propanediol;

[0035] (3) In the anaerobic work box, a single colony was isolated by streaking on the plate, and the strains capable of producing 1,3-propanediol and culture characteristics, and the colony morphology were selected for 16SrDNA sequence analysis and identification, and finally obtained A Clostridium butyricum M01 capable of producing 1,3-propanediol;

[0036] The above-mentioned Clostridium proliferation liquid medium is: tryptone, 10g / L; beef extract, 10g / L; yeast powder, 3g / L; glucose, 5g / L; NaC...

Embodiment 3

[0048] Utilize the Clostridium butyricum M01 that embodiment 1 obtains to produce 1,3-propanediol in 5L fermentor, comprise the steps:

[0049] (1) Seed culture: use a sterile syringe to inoculate 1-10 mL of Clostridium butyricum M01 bacterial solution into a serum bottle containing 50-100 mL of seed medium, and culture on a shaker at 33-39°C and 120-200 rpm for 8-16 hours , to obtain the primary seed culture solution;

[0050] (2) Expanded cultivation of seeds: use a sterile syringe to inoculate 1-10 mL of seed solution into a serum bottle containing 50-100 mL of seed medium, and culture on a shaker at 33-39°C and 120-200 rpm for 2-8 hours to obtain two grade seed culture solution;

[0051] (3) Fermentation tank culture: Under the conditions of 37°C, 150rpm, and nitrogen gas 0.5vvm, the primary or secondary seed culture liquid and the fermentation medium were cultured in a fermenter at a volume ratio of 1:10, and the amount of fermentation medium was is 2L, and in the ferme...

Embodiment 4

[0054] Utilize the Clostridium butyricum M01 that embodiment 1 obtains to produce 1,3-propanediol in 100L fermentor, comprise the steps:

[0055] (1) Seed culture: use a sterile syringe to inoculate 1-10 mL of Clostridium butyricum M01 bacterial solution into a serum bottle containing 50-100 mL of seed medium, and culture on a shaker at 33-39°C and 120-200 rpm for 8-16 hours , to obtain the primary seed culture solution;

[0056] (2) Expanded cultivation of seeds: use a sterile syringe to inoculate 1-10 mL of seed solution into a serum bottle containing 50-100 mL of seed medium, and culture on a shaker at 33-39°C and 120-200 rpm for 2-8 hours to obtain two grade seed culture solution;

[0057] (3) Fermentation tank culture: Under the conditions of 37°C, 250rpm, and nitrogen gas 2.5vvm, the primary or secondary seed culture liquid and the fermentation medium were cultured in a fermenter at a volume ratio of 1:20, and the amount of fermentation medium was is 60L, and in the fe...

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Abstract

The invention discloses clostridium butyricum and an application thereof. The clostridium butyricum is preserved in the China General Microbiological Culture Collection Center in September 1, 2014 and has a preservation number of CGMCC NO.9626. The clostridium butyricum disclosed by the invention is easy to culture, is relatively high in conversion rate and production intensity of 1,3-propylene glycol, and has relatively high tolerance to a substrate and a product.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a Clostridium butyricum and application thereof. Background technique [0002] 1,3-propanediol (1,3-propanediol, abbreviation: 1,3-PD) is an important chemical raw material, as a typical diol compound, it is widely used in lubricants, dyes, inks, antifreeze Agents, solvents and pharmaceutical industries, and can also synthesize polymers such as polyurethane. The dehydration and dehydrogenation of 1,3-propanediol can produce products with chemical properties similar to tetrahydrofuran and γ-butyrolactone, such as oxetane, and produce products similar to polytetramethylene ether glycol ( PTMEG), or as a reactive solvent in coatings. 1,3-propanediol and terephthalic acid can synthesize polytrimethylene terephthalate (PTT), which makes 1,3-propanediol attract the attention of the world. PTT is a new fiber-forming polyester polymer material that has achie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/18C12R1/145
CPCC12N1/20C12P7/18C12N1/205C12R2001/145
Inventor 方柏山邱隆辉王世珍
Owner XIAMEN UNIV
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