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Method for preparing selaginella doederleinii hieron polysaccharide extract product and application thereof

A polysaccharide extraction and extract technology, which is applied in the field of pharmacology to achieve the effect of abundant resources and remarkable medicinal effect

Inactive Publication Date: 2015-04-22
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few literatures and patent reports on the research of polysaccharides from Cypress polysaccharides
Moreover, there is no medicine or pharmaceutical composition about polysaccharides from Cypress polysaccharides on the market.

Method used

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  • Method for preparing selaginella doederleinii hieron polysaccharide extract product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of Cypress polysaccharide extract

[0018] (1) Degreasing: take the dried whole herb of Cypress cypress, soak it with 8 times the amount of ethanol, reflux twice, filter, and evaporate the organic solvent to get the medicinal residue; (2) Extraction: soak the above medicinal residue with 10 times the amount of water, then soak in a water bath Extract 3 times, 1 hour each time, filter, concentrate the filtrate to 1.0g crude drug per ml; (3) Alcohol precipitation: add an appropriate amount of ethanol to the above concentrated solution until the ethanol content is 80%, place in ice bath for 12 hours, Filtration, precipitation and freeze-drying of the crude polysaccharide extract I; (4) protein removal: dissolve the above crude polysaccharide extract I in water to prepare a 0.05 g / ml crude product I solution, add 1 volume of chloroform-n-butanol ( V / V= 4:1) mixed solution, layered after stirring, layered after stirring, the upper layer was repeated...

Embodiment 2

[0019] Example 2 Preparation of Cypress polysaccharide extract

[0020] (1) Degreasing: Take the dried whole herb of Cypress cypress, soak it with 10 times the amount of 80% ethanol, reflux twice, filter, evaporate the organic solvent to get the medicinal residue; (2) Extraction: soak the above medicinal residue with 8 times the amount of water , extracted twice in water bath, 1 hour each time, filtered, concentrated filtrate to 1.5g of crude drug per ml; (3) Alcohol precipitation: Add appropriate amount of ethanol to the above concentrated solution until the ethanol content is 80%, place in ice bath for 24 hours, filtered, precipitated and freeze-dried polysaccharide extract crude product I; (4) protein removal: dissolve the above polysaccharide extract crude product I in water to prepare a 0.1g / ml crude product I solution, add 3 times the volume of chloroform-n-butyl Alcohol (V / V= 4:1) mixed solution, stirred and layered, the upper layer was repeated 8 times, added a...

Embodiment 3

[0021] Example 3 Preparation of Cypress polysaccharide extract

[0022] (1) Degreasing: Weigh 4 kg of cypress herb (dry product, whole plant), soak in 8 times the amount of 80% ethanol for 3 hours (W / V), reflux at 85°C for 5 hours, twice. Filter, spread the dregs evenly, and evaporate the ethanol; (2) Extraction: evaporate the dregs above until there is no alcohol smell, add 4 times the amount of distilled water (W / V), soak for 12 hours, extract in a 50°C water bath for 2 hours, twice. Suction filtration through a four-layer gauze Buchner funnel, collect the filtrates, combine them, and concentrate to 3000ml by rotary evaporation at 60°C; (3) Alcohol precipitation: add about 9000ml of absolute ethanol to the above concentrated solution, and adjust to 80% ethanol content with an alcohol hydrometer , placed in ice bath for 24 hours, flocculent precipitate appeared, filtered with four layers of gauze Buchner funnel, precipitated freeze-dried crude polysaccharide extract...

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Abstract

The invention relates to a method for preparing a selaginella doederleinii hieron polysaccharide extract product and an application thereof. The selaginella doederleinii hieron polysaccharide extract product is prepared by the steps of degreasing, extraction, alcohol precipitation, protein removal, monosaccharide and oligosaccharides removal, gel column purification and the like. The polysaccharide content in the polysaccharide extract product is more than 90%. The polysaccharide extract product has obvious antioxidation activity, and can be used for preparing in-vitro antitumor medicine or pharmaceutical composition.

Description

technical field [0001] The invention belongs to the field of pharmacology, and in particular relates to a preparation method and application of polysaccharide extracts of cypress polysaccharides. Background technique [0002] Ishigami ( Selaginella doederleinii Hieron) is a commonly used Chinese medicinal material, belonging to the family Pteridophyta (Pteridophyta) Selaginaceae ( Selaginellaceae ) Selaginella ( Selaginella ) dried whole grass of the plant. It is also known as dark green selaginella, big leafy vegetable, water cypress branch, etc. It is mainly produced in Yunnan, Guizhou, Guangxi, Zhejiang, Fujian, Taiwan and other places. Its nature and taste are sweet, slightly bitter, astringent, and cool. In traditional Chinese medicine, a total of six Selaginella plants have been included in the classics, among which Cypress is one of the medicinal materials commonly used to treat tumors. Traditional Chinese medicine believes that Shishangbai has the dual function...

Claims

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Application Information

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IPC IPC(8): A61K31/715C08B37/00A61P35/00
CPCA61K31/715C08B37/0003
Inventor 姚宏林新华王盼盼范思思陈雨巍王鑫赖璐璐
Owner FUJIAN MEDICAL UNIV
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