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Preparation method of porous ferroferric oxide composite nanometre microspheres efficiently loaded with pharmorubicin

A technology of ferric oxide and epirubicin, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc., which can solve the problems of poor drug release effect, cumbersome preparation process, and lack of targets. tropism and other issues, to achieve the effect of low cost, simple process and good biocompatibility

Inactive Publication Date: 2015-03-25
JINLING INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the shortcomings of existing drug carriers such as lack of targeting, low loading rate, poor drug release effect, cumbersome preparation process, and high cost, and provide a magnetically targeted drug carrier material capable of efficiently loading epirubicin Preparation

Method used

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  • Preparation method of porous ferroferric oxide composite nanometre microspheres efficiently loaded with pharmorubicin
  • Preparation method of porous ferroferric oxide composite nanometre microspheres efficiently loaded with pharmorubicin
  • Preparation method of porous ferroferric oxide composite nanometre microspheres efficiently loaded with pharmorubicin

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Experimental program
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Embodiment 1

[0036] Dissolve 1.35 g ferric chloride in 72 ml 1,2-propylene glycol, add 0.5 g sodium dodecylbenzenesulfonate and 0.9 g urea to form a mixed solution. After being placed in a reactor and sealed, it was placed in a muffle furnace for 6 h at 220 °C. The product was washed alternately with deionized water and absolute ethanol. Finally, dry at 50 °C to obtain nanoporous Fe 3 o 4 Magnetic microspheres, sealed and kept for later use.

Embodiment 2

[0038] The nanoporous Fe of 60 mg embodiment 1 3 o 4 Magnetic microspheres were dissolved in 90 ml ethanol, 9 ml H 2 O and 3 ml ammonia water mixed solution, placed in a three-necked flask, ultrasonically dispersed for 30 min, then added 1 ml 3-aminopropyltrimethoxysilane, and continued to stir at 25 °C for 8 h. The product was washed with deionized water and absolute ethanol, and dried under vacuum at 50 °C to obtain Fe 3 o 4 -NH 2 Nanoparticles. Dissolve 15 mg EDAC HCl (1-ethyl-(3-dimethylaminopropyl)-carbodiimide hydrochloride) and 18 mg NHS (N-hydroxysuccinimide) in 10 ml PBS (50mM pH=7.4), add 20mg Fe 3 o 4 -NH 2 Nanoparticles were ultrasonically dispersed for 10 min, then 50 ml of 1g / L sodium alginate was added, mechanically stirred for 24 h at room temperature, the product was washed with deionized water, and finally dried at 50 °C to obtain sodium alginate-modified Fe 3 o 4 SA nano-microspheres, sealed and kept for later use.

[0039] The nanoporous Fe of 65...

Embodiment 3

[0041] 5 mg of epirubicin was dissolved in 30 ml of distilled water, placed in a three-necked flask, and the sodium alginate-modified Fe prepared in Example 2 was added dropwise under the action of mechanical stirring. 3 o 4 SA magnetic nanospheres (40 mg, 8 ml water, 0.5% mass fraction), stirred for 6 h, then added 10 g / L CaCl 2 100 ml of the solution was reacted for 30 min and centrifuged to obtain drug-loaded magnetic nanospheres. The product was washed with distilled water, and finally dried at 50 °C to obtain the final product Fe 3 o 4 SAEPI, sealed and kept for later use.

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Abstract

The invention discloses a preparation method of porous ferroferric oxide composite nanometre microspheres efficiently-loaded with pharmorubicin. The preparation method comprises the following steps: dissolving ferric salts in polyhydric alcohols, adding a surfactant and urea to form a mixed solution, placing the mixed solution in a reaction device and sealing, then reacting at 150-250 DEG C, after that, washing and drying the precipitate, then carrying out amination on porous Fe3O4 microspheres, adding a condensing agent and then carrying out an aminocarboxylic condensation reaction to realize the modification of sodium alginate for the Fe3O4 microspheres, finally loading pharmorubicin by taking the microspheres as carriers. The invention develops a preparation method of a magnetic-targeted medicine carrier which is simple in process, low in cost, high in loading rate, low in toxicity and good in biocompatibility, and the preparation method is of great significance on the popularization of the application of the magnetic-targeted medicine carrier in treatment on breast cancer.

Description

technical field [0001] The invention relates to the field of magnetic nanometer materials, in particular to a method for synthesizing highly efficient loaded epirubicin porous iron ferric oxide composite nanometer microspheres. Background technique [0002] Magnetic nanomaterials are composite materials with magnetic properties and special structures. They have extensive applications in the fields of bioengineering (immobilized enzymes), biomedicine (targeted drug delivery, clinical diagnosis), and cytology (cell separation, cell labeling). application. Ferric oxide (Fe 3 o 4 ) is the most typical type of magnetic nanomaterials, using porous Fe 3 o 4 Loading drugs can not only make composite microsphere Fe 3 o 4 EPI has magnetic targeting, and can make the drug have a good controlled release effect. In recent years, magnetically targeted chemotherapy has become a relatively cutting-edge treatment technology in the clinical treatment of breast cancer. Because of its l...

Claims

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Application Information

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IPC IPC(8): A61K47/04A61K47/36A61K9/14A61K31/704A61P35/00
Inventor 张小娟郝凌云魏智勇田湘萍朱星群李景顾菁菁
Owner JINLING INST OF TECH
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