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IgM (Immune Globulin M) immune turbidimetry test kit

A technology for immunoglobulin and detection kits, which is applied in the field of immunoglobulin IgM immunoturbidimetric detection kits, can solve problems such as low accuracy and sensitivity, limited popularization and application, and poor stability of detection reagents, and achieves Effects of enhanced stability and improved analytical sensitivity

Active Publication Date: 2015-03-11
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ordinary immunoglobulin IgM immunoturbidimetric assay reagents are not stable, and the accuracy and sensitivity are not high, which limits its clinical application.

Method used

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  • IgM (Immune Globulin M) immune turbidimetry test kit
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  • IgM (Immune Globulin M) immune turbidimetry test kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A conventional detection kit for immunoglobulin IgM immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0024] Wherein the reagent R1 consists of:

[0025] pH 7.6 Tris buffer 18.16 mmol / L

[0026] Sodium chloride 123.20 mmol / L

[0027] Polyethylene glycol 400 6%

[0028] Reagent R2 consists of:

[0029] Tris buffer pH 7.6 18.16 mmol / L

[0030] Goat anti-human IgM antibody 30ml / L

[0031] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0032] Add 2 μl of physiological saline, sample or calibrator, then add 250 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 50 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0033] The calibrator used in this example is the IgM calibrator produced by Jiufeng Runda Biotechnology Co., Ltd.

Embodiment 2

[0035] An immunoglobulin IgM immune turbidimetric detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0036] Wherein the reagent R1 consists of:

[0037] pH 7.6 Tris buffer 18.16 mmol / L

[0038] Sodium chloride 123.20 mmol / L

[0039] Polyethylene glycol 400 6%

[0040] Silica coated magnetic nanoparticles 0.1%

[0041] Reagent R2 consists of:

[0042] Tris buffer pH 7.6 18.16 mmol / L

[0043] Goat anti-human IgM antibody 30ml / L

[0044] Bovine serum albumin 20g / L

[0045] Kathon-CG 0.05%

[0046] Concrete determination method is with embodiment 1.

Embodiment 3

[0048] An immunoglobulin IgM immune turbidimetric detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0049] Wherein the reagent R1 consists of:

[0050] pH 7.6 Tris buffer 18.16 mmol / L

[0051] Sodium chloride 123.20 mmol / L

[0052] Polyethylene glycol 400 6%

[0053] Silica coated magnetic nanoparticles 1%

[0054] Reagent R2 consists of:

[0055] Tris buffer pH 7.6 18.16 mmol / L

[0056] Goat anti-human IgM antibody 30ml / L

[0057] Bovine serum albumin 30g / L

[0058] Kathon-CG 0.05%

[0059] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses an IgM (Immune Globulin M) immune turbidimetry test kit, and belongs to the technical field of clinical in-vitro test reagents. The kit disclosed by the invention comprises a reagent R1, a reagent R2 and a calibration material. The stability and the analysis sensitivity of the kit are effectively improved by adding a certain amount of silicon dioxide-cladded magnetic nano-particles into the reagent R1 and adding a certain amount of bovine serum albumin and Kathon-CG into the reagent R2; the linear range of the kit is also larger; the accuracy of each reagent is high; popularization and application of the kit in markets are facilitated.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an immunoglobulin IgM immunoturbidimetric detection kit. Background technique [0002] Immunoglobulins are composed of heavy chains and light chains. According to the chemical structure of the constant region of the heavy chain (H chain), the corresponding immunoglobulins are divided into various types, namely immunoglobulin M (IgM), Immunoglobulin D (IgD), Immunoglobulin A (IgA), Immunoglobulin E (IgE), and Immunoglobulin G (IgG). [0003] Human immunoglobulin M (IgM) exists in human serum, accounting for 5-10% of the total serum immunoglobulins, and the serum concentration is about 1mg / ml. It is mainly synthesized by plasma cells in the spleen. One IgM molecule is composed of 5 single The body is connected into a pentamer through the J chain, which has a high antigen binding value and is also the largest molecular weight among the five classes of i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/543G01N33/6854
Inventor 甘宜梧董雯谭柏清谢清华王绮李静包兴艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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