Fluorescence analysis method for simultaneously measuring bromate and chlorite

Abstract

The invention discloses a fluorescence analysis method for simultaneously measuring bromate and chlorite. The method comprises the following steps: firstly, measuring the respective fluorescence quenching kinetic curves of the bromate and the chlorite to a fluorescent reagent; then selecting two reaction time points t1 and t2, respectively measuring fluorescence intensity variations produced by the reaction of the bromate and the chlorite with the fluorescent reagent at the t1 and the t2, and measuring the respective linear regression equations of the concentration contents to the fluorescence intensity quenching ratios (delta F / F) of the bromate and the chlorite at the t1 and the t2; combining the respective linear regression equations of the concentration contents to the fluorescence intensity quenching ratios (delta F / F) of the bromate and the chlorite, obtained at the t1 and the t2, and integrating to obtain a fluorescence variation rate regression equation group at the t1 or the t2; carrying out operation on the fluorescence variation rate regression equation groups which are obtained at the t1 and the t2, and calculating to obtain the contents of the bromate and the chlorite. The method is simple, component separation is not required and the method can be used for rapid and sensitive analysis of trace bromate.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a fluorescence analysis method for simultaneously measuring bromate and chlorite. Background technique [0002] Bromate is carcinogenic, teratogenic, causes nephropathy and strong reproductive toxicity. The World Health Organization has banned the use of bromate in food and beverages. my country's raw water enterprises generally adopt the low-cost and efficient ozone sterilization process, and the bromate produced by the reaction between bromine-containing substances and ozone inevitably exists, resulting in the increasingly prominent problem of bromate residues in drinking water. [0003] The content of bromate in drinking water is low, and gas chromatography and liquid chromatography are detected after derivatizing bromate, and the operation is relatively cumbersome. At present, the detection method of bromate in drinking water mainly adopts ion chromatography-con...

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Problems solved by technology

However, trace amounts of chlorine in water or chlorine-containing disinfectants can produce strong oxidizing chlorite under the action of ozone, and chlorite has a strong oxidation quenching effect on conventionally used fluorescent reagents. The principle of bromate quenching fluorescent reagent is the same, and the effect is very strong, which will definitely cause great interference to the accurate determination of trace bromate

[0004]At present, my country’s National Standards Committee sets the bromate limit in drinking water as 10.0μg·L-1. The killing method has no resolution for the oxidation of bromate and chlorite. In order to accurately determine bromate, ion chromatography or special silver column must be used to separate chlorite, which is complicated to operate.

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