Synchronous detection method and device for beta-carotene and vitamin A

A carotene and synchronous detection technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult elution and low polarity of beta-carotene, and achieve the effect of avoiding clogging

Pending Publication Date: 2021-12-07
杨艳玲 +1
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in terms of chromatographic behavior, β-carotene has the characteristics of low polarity, and it is difficult to elute compared with other organic compounds

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synchronous detection method and device for beta-carotene and vitamin A
  • Synchronous detection method and device for beta-carotene and vitamin A
  • Synchronous detection method and device for beta-carotene and vitamin A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] figure 1 The method of synchronization detection method β- carotene and vitamin A according to an embodiment of the present invention. FIG. In the present embodiment, it is possible to complete quantitative β- carotene and vitamin A method of analysis using an internal standard. like figure 1 As shown, the synchronization detection method may comprise the steps of:

[0073] S100, the establishment of β- carotene and vitamin A standard curve.

[0074] Wherein the standard curve refers to as a reference point in accordance with standard and various concentrations of the corresponding peak area, using the method of least squares regression analysis, and the like, is formed to establish a concentration - area under the curve peak.

[0075] In the present embodiment, the ratio between the concentration of the internal standard of known concentration and β- carotene and the peak area ratio as a reference point corresponding to construct a standard curve of β- carotene, and the k...

Embodiment 2

[0115] image 3 A method of establishing β-carotene and vitamin A for the embodiment of the present invention. In the present embodiment, the standard curve of β-carotene is referred to as "first standard curve", and the standard curve of vitamin A is referred to as "second standard curve". like image 3 As shown, its establishment of a standard curve process is as follows:

[0116] S110, configure a plurality of different concentrations of first standard solutions and second standard solutions.

[0117] Among them, the first standard solution is a solution containing a known concentration of beta-carotene and Sudanese I (internal standard). The second standard solution is a solution containing a known concentration of vitamin A and Sudanese I (internal standard).

[0118] In a preferred embodiment, the above-described first standard solution and the second standard solution can use a bovine serum protein solution as a blank matrix. The standard curve drawn in this manner is more a...

Embodiment 3

[0140] Based on the synchronization detection method provided by Example 1 and Example 2, the embodiment of the present invention also provides a synchronous analysis method of beta-carotene and vitamin A based on internal standard method. This synchronization analysis method can include the following steps:

[0141] 1. About the selected internal standard:

[0142] 11) 20 μl of the Sudanese I solution (50 ppm dissolved in methanol) was added to 200 μl plasma, and the sample to be tested (experimentation of 6 sets of plasma sample) was obtained.

[0143] 12) Add 600 μl of protein precipitating to the sample to be tested, mixed with uniform oscillate for 10 minutes, and precipitate the protein in the biological sample to be tested to form a sample of the solid-liquid mixed state.

[0144] 13) Add 600 μl of the extractant to the sample to be tested in a solid-liquid mixed state, and mix well after oscillation for 10 minutes.

[0145] 14) After oscillating for 10 minutes, it was trea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to view more

Abstract

The embodiment of the invention provides a synchronous detection method and device for beta-carotene and vitamin A. The method comprises the following steps: establishing a standard curve of beta-carotene and vitamin A; adding an internal standard substance into a biological sample to be detected; adding a protein precipitant, fully and uniformly mixing, and oscillating to precipitate the protein to form a solid-liquid mixed sample; extracting target components (beta-carotene and vitamin A); performing high performance liquid chromatography detection on the target components; and calculating the concentrations of the beta-carotene and the vitamin A in the biological sample to be detected according to the standard curve and a detection result. By adopting a mode of combining extraction and high performance liquid chromatography detection, synchronous analysis of vitamin A and beta-carotene in a biological sample can be realized, and respective elution is not needed. In addition, proper pretreatment is applied to the biological sample, so that impurities in the biological sample are prevented from blocking a chromatographic column.

Description

[Technical field] [0001] The present invention relates to organic detection technology, and particularly relates to a synchronization detecting method and apparatus for detecting β- carotene and vitamin A. 【Background technique】 [0002] Retinol, also known as vitamin A, one of 13 human essential vitamin. It maintains in human growth and development, immune system, and inhibit the growth of tumor cells in the visual system is important. The body of vitamin A in vitamin A foods are usually obtained from the intake contains. [0003] Wherein, in animal foods, vitamin A, retinol esters mainly exists in the way, it may be degraded to retinol in the small intestine. In plant food, mainly in the form of vitamin A carotenoids exist, most of which is present in the form of β- carotene, it can be converted to retinol in the enzyme catalysis. [0004] When the body is suffering from certain diseases, intake of vitamin A may be reduced, or vitamin A consumption will increase, leading to vit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045G01N2030/062
Inventor 杨艳玲董慧陈泽辉马乐杨元凯
Owner 杨艳玲
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap