Ionic liquid bridged hybrid silicon oxide serving as water-rich chromatographic stationary phase as well as preparation method and application of ionic liquid bridged hybrid silicon oxide

Abstract

The invention discloses ionic liquid bridged hybrid silicon oxide serving as a water-rich chromatographic stationary phase as well as a preparation method and application of the ionic liquid bridged hybrid silicon oxide. The invention provides a novel hydrophilic stationary phase PMO-ILs-Au NPs which is environmentally friendly. The novel stationary phase has the characteristics of HILIC and PALC,and same as the HILIC, the PALC can retain polar compounds. The stationary phase can separate organic acids, biogenic amines and other polar compounds, and has the advantages of high separation degree and favorable selectivity. The invention also establishes a PALC-MS / MS detection method of eight biogenic amine compounds in aquatic products, the operation is simple, accurate and rapid, the separation effect is good, synchronous analysis of the eight biogenic amine compounds can be realized, and the detection requirements of common biogenic amine compounds in food can be met.

Description

technical field [0001] The invention belongs to the field of food testing, and relates to an ionic liquid bridging hybrid silicon oxide used as a stationary phase of water-rich chromatography, a preparation method and application thereof. Background technique [0002] Food additives are widely used in food production and are an important part of the modern food industry. According to national regulations, there are only more than 2,000 food additives allowed to be used in food, and some unscrupulous manufacturers lack integrity and add some illegal ingredients to processed foods to increase the color and taste of food or counterfeit main ingredients. The proliferation of illegal additives has brought great harm to my country's food safety. In recent years, food safety issues have been exposed frequently, such as dicyandiamide and melamine incidents in dairy products, "leather milk" incidents, malachite green incidents in aquatic products, clenbuterol in feed, sodium copper ...

AI Technical Summary

Problems solved by technology

[0005]In the PALC mode, a high proportion of water needs to be used as the mobile phase, which is a great test for the service life of the separation material, and the following problems need to be solved : (1) General stationary phases, such as C18, after using rich water mobile phases for a long time, the column efficiency will be seriously reduced, especially when there are silanols exposed

(2) The pH range that can be used is narrow; when pH>8, silica gel is easy to dissolve; when pH<2, the bonded silica gel stationary phase will be lost, so that the retention characteristics and peak shape of the analyte change, and the selectivity of the chromatographic column reduce

(3) When analyzing alkaline substances, the exposed silanic hydroxyl groups on the surface of silica gel have an irreversible adsorption effect on alkaline substances, especially N-containing compounds, and easily denature biological macromolecules, especially polypeptides, proteins, etc., resulting in non-specific adsorption. , resulting in severe tailing of chromatographic peaks and lower recovery

Images